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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 16,276 results for: nes

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Showing: 3821 - 3840 of 16276 results

  4. pX330A_D10A-1x6

    Plasmid
    #58776
    Purpose
    Expresses Cas9 nickase and gRNA
    Depositor
    Takashi Yamamoto
    Publication
    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanized S. pyogenes Cas9 (D10A) nickase
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Mutation
    D10A nickase-converting mutation
    Promoter
    CBh
    Available since
    Nov. 7, 2014
    Availability
    Academic Institutions and Nonprofits only
    58776-plasmid-map-sequence-id-91086

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  9. pLV-UbC-Tet1v4-dCas9

    Plasmid
    #232180
    Purpose
    Tet1v4-dCas9 from Nuñez et al. 2021, in a lentiviral cassette with blasticidin resistance, for expression in mammalian cells
    Depositor
    Charles Gersbach
    Publication
    Rohm et al Cell Genom. 2025 Feb 12;5(2):100770. doi
    Insert
    Tet1 catalytic domain - XTEN80 linker - dead Cas9 - 2A - BlastR (TET1 S. pyogenes, Human)
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    hUbC
    Available since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    232180-plasmid-map-sequence-id-462961

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  10. pCAG-postASAP

    Plasmid
    #178792
    Purpose
    Expresses voltage sensor targeted to dendrites and spines
    Depositor
    Rafael Yuste
    Publication
    Cornejo et al Science. 2022 Jan 7;375(6576):82-86. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    FingR.PSD95 and ASAP2f
    Expression
    Mammalian
    Mutation
    mutations in amino acids L146G, S147T N149R, S150…
    Promoter
    CAG
    Available since
    Dec. 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    178792-plasmid-map-sequence-id-350047

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  11. pCAG-scFvGCN4sfGFP-VP64-GB1

    Plasmid
    #141417
    Purpose
    Express scFvGCN4-sfGFP-VP64 fusion protein for dCas9-SunTag mediated targeted gene activation
    Depositor
    Izuho Hatada
    Publication
    Morita et al Int J Mol Sci. 2020 Feb 25;21(5). pii: i
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    scFvGCN4sfGFP-VP64-GB1
    Tags
    HA and sfGFP
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    June 22, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    141417-plasmid-map-sequence-id-300656

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  12. pMTMVi-M

    Plasmid
    #118757
    Purpose
    Intermediate plasmid to build tobacco mosaic virus recombinant clones that move systemically throughout the plant.
    Depositor
    Jose-Antonio Daros
    Publication
    Shi et al Plant Biotechnol J. 2018 Nov 22. doi: 10
    Insert
    Tobacco mosaic virus cDNA fragment with a cloning polylinker
    Use
    Intermediate to build tobacco mosaic virus recomb…
    Available since
    Dec. 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    118757-plasmid-map-sequence-id-231795

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  16. SECURE miniABEmax-K20A/R21A (pJUL1774)

    Plasmid
    #131312
    Purpose
    CMV promoter expression plasmid for bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with K20A/R21A mutations).
    Depositor
    Keith Joung
    Publication
    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bpNLS-TadA7.10(K20A/R21A)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    131312-plasmid-map-sequence-id-254654

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  18. YE2-BE4max

    Plasmid
    #138156
    Purpose
    C-to-T base editor
    Depositor
    David Liu
    Publication
    Doman et al Nat Biotechnol. 2020 Feb 10. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    rAPOBEC1(YE2)-nSpCas9-UGI-UGI
    Tags
    NLS
    Expression
    Mammalian
    Mutation
    Within rAPOBEC1 W90Y + R132E; within Cas9 D10A
    Promoter
    CMV
    Available since
    Feb. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    138156-plasmid-map-sequence-id-272343

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Showing: 3821 - 3840 of 16276 results