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Plasmid#127557PurposeCRISPaint universal donor plasmid for gene exon insertion. Encodes T2A-sfGFP-SV40 and 3xP3-RFP visible eye marker.DepositorInsertsfGFP
UseCRISPRExpressionInsectAvailable sinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKII-soCoChR-GFP
Plasmid#107711PurposeA somatic channelrhodopsin, which enables single cell, single millisecond resolution optogenetics. CaMKII promoterDepositorInsertsoCoChR-GFP
UseAAVTagsEGFP and KA2ExpressionMammalianPromoterCaMKIIAvailable sinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
tdTomato-Fibrillarin-7
Plasmid#58093PurposeLocalization: Nuclear - Nucleolus, Excitation: 554, Emission: 581DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFibrillarin (FBL Human)
TagstdTomatoExpressionMammalianMutationR36Q, S66FPromoterCMVAvailable sinceDec. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
EBFP2-Fibrillarin-7
Plasmid#55241PurposeLocalization: Nucleoli, Excitation: 383, Emission: 448DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFibrillarin (FBL Human)
TagsEBFP2ExpressionMammalianMutationR36Q, S66FPromoterCMVAvailable sinceOct. 10, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
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pAAV-UBC-XRI-HA
Plasmid#178056PurposeExpression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with HA tag, under UBC promoter.DepositorInsertXRI-HA
UseAAVTagsHA-MBPExpressionMammalianPromoterHuman ubiquitin C (UBC) promoterAvailable sinceOct. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
vYM112_PB-pEF1a-optiPIN2-HA(Hygro)
Plasmid#160045PurposeFor expressing the active auxin pump PIN2DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPIN2
UseSynthetic BiologyTagsHAExpressionMammalianMutationcodon optimizedPromoterpEF1sAvailable sinceApril 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCS2-mCherry_SNAP_SNAPV_PACT_Active
Plasmid#173072PurposeActive SNAP tag at the centrosomeDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-SNAP-SNAP-Varied-PACT
TagsmCherry-SNAP-SNAP-VariedExpressionMammalianPromoterCMV IE94Available sinceAug. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pES001 MBP-GSK3β-HA-His
Plasmid#154010PurposeExpresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coliDepositorAvailable sinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-P4:gs:nls:VPR
Plasmid#135983PurposeExpresses the P4 coiled coil fused to the VPR transcriptional activation domain in mammalian cellsDepositorInsertP4 coiled-coil fused to the VPR activation domain
ExpressionMammalianPromoterCMVAvailable sinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
MOM603
Plasmid#133242Purposeexpresses full-length human KIF1A fused with mScarlet and StrepII tag in insect cellsDepositorInsertkif1a (KIF1A Human)
TagsStrepII tag and mScarletExpressionInsectPromoterpolyhedrin promoterAvailable sinceJan. 13, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAD29_hIRG1_4-461_pvp008
Plasmid#124843PurposeExpresses human CAD (Irg1) in E.coliDepositorInsertcis-aconitate decarboxylase (ACOD1 Human)
TagsStrepTag and TEV siteExpressionBacterialMutationdeleted amino acids 1-3 and 462-end.PromoterT7Available sinceSept. 16, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
IY601: pMVP/Lenti/Blast-DEST
Plasmid#121849PurposepMVP destination vector, empty Lentivirus vector backbone w/ Blast selection cassetteDepositorTypeEmpty backboneUseLentiviral; Pmvp gateway destination vectorAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
CLTA-GFP HDRT Source (pTR 153)
Plasmid#112016PurposeDNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFPDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCLTA-GFP HDRT (CLTA Human)
UseCRISPR and Synthetic BiologyAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
tCRMod-GGSGGS5
Plasmid#111760PurposeExpresses soluble Clover-mRuby2 tension sensor module with 5-repeat GGSGGS extensible domainDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertClover-GGSGGS5-mRuby2 fusion
ExpressionMammalianMutationResidues 1-228 of Clover, residues 1-3 deleted fr…PromoterCMVAvailable sinceJuly 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBV65
Plasmid#108363PurposeA nourseothricin resistance marker used to construct gene disruptions in Candida glabrata. The natMX6 marker can be evicted by growth on media lacking methionine and containing estradiol.DepositorInsertloxP-natMX6-CgMET3prom-cre-loxP
UseGene disruption marker for candida glabrataPromoterC. glabrata MET3Available sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMXs3-NRAS G13D
Plasmid#86144PurposeRetroviral vector expressing NRAS(G13D)-IRES-RFPDepositorAvailable sinceFeb. 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-loxP-P2A-EGFP-loxP
Plasmid#188773PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionPromoterEF1aAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDF0250 DisCas7-11 full locus with Golden gate acceptor site for spacer
Plasmid#172501PurposeEncodes the DisCas7-11 full locus along with a DR and golden gate site for cloning spacersDepositorInsertDisCas7-11 full locus with Golden gate acceptor site for spacer
ExpressionBacterialAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
Cbh_v5 AAV-saCBE_KKH C-terminal
Plasmid#137184PurposeAAV genome: expresses the C-terminal of S. aureus v5 AAV-CBE, and U6-sgRNA (KKH variant).DepositorInsertv5 AAV-saCBE_KKH C-terminal
UseAAVMutationE782K;N968K;R1015H conferring recognition of NNNR…PromoterCbhAvailable sinceJan. 31, 2020AvailabilityAcademic Institutions and Nonprofits only