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pQlinkH-PTPIP51(236-470)

Plasmid

#170530

Purpose

Expresses Cleavable His-tagged PTPIP51 TPR domain (236-470) in E. Coli

Depositor

Byung Il Lee

Article

Yeo et al EMBO Rep. 2021 Jun 4;22(6):e51323. doi:

Insert

Protein tyrosine phosphatase interacting protein 51 (236-470) (RMDN3 Human)

Tags

His tag

Expression

Bacterial

Promoter

T5 promoter

Available Since

July 27, 2021

Availability

Academic Institutions and Nonprofits only

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LR (hsp70I: cPLA2-mK2)

Plasmid

#162564

Purpose

Measuring cPLA2 activation in live cells after heat shock.

Depositor

Philipp Niethammer

Article

Enyedi et al Cell. 2016 May 19;165(5):1160-1170. doi:

Inserts

Heat shock protein 70 (HSPA1A Human)
cytosolic phospholipase a2 (pla2g4aa Zebrafish)

Use

Gateway donor vector

Tags

mK2

Promoter

cmlc2

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

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GFP-Miro1 (1-177 fused to 562-618)

Plasmid

#127627

Purpose

First GTPase domain of human Miro1 fused to C-terminus

Depositor

Josef Kittler

Article

Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

Insert

Miro1 (RHOT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

1-177 fused to 562-618

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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GFP-Miro1 (412-618)

Plasmid

#127628

Purpose

GTPase2 domain of human Miro1 including transmembrane domain and C-terminal amino acids

Depositor

Josef Kittler

Article

Covill-Cooke et al EMBO Rep. 2020 Feb 5;21(2):e49865. doi:

Insert

Miro1 (RHOT1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

412-618 only

Available Since

Oct. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pTCO2-mTDGi.2

Plasmid

#149431

Purpose

miniTdg gene, SUMOylation mutant

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Tdg (Tdg Mouse)

Use

Cre/Lox

Expression

Mammalian

Mutation

K341A SUMOylation mutant, SNPs of Tdg gene in OLA…

Promoter

Tdg promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pTCO2-mTDGi.1

Plasmid

#149430

Purpose

miniTdg gene, catalytic mutant

Depositor

Primo Schaer

Article

Schwarz et al F1000Res. 2020 Sep 9;9:1112. doi: 10.126

Insert

Tdg (Tdg Mouse)

Use

Cre/Lox

Expression

Mammalian

Mutation

N151A catalytic mutant, SNPs of Tdg gene in OLA/1…

Promoter

Tdg promoter

Available Since

Sept. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pLB(N)CX-FLAG-CH3 Del33

Plasmid

#133724

Purpose

Retroviral vector with CMV promoter driving mutant form of CH3 domain fragment of human p300 (EP300), with N-terminal FLAG tag.

Depositor

James A. DeCaprio

Article

Borger et al J Virol. 2006 May . 80(9):4292-303.

Insert

EP300 (EP300 Human)

Use

Retroviral

Tags

Flag

Mutation

partial (not complete) p300 fragment, AT and CH3 …

Promoter

CMV

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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pLB(N)CX AT-CH3-FLAG-HA WY

Plasmid

#133725

Purpose

Retroviral vector with CMV promoter driving mutant form of acetyltransferase and CH3 domains fragment of human p300 (EP300) (WY1466-1467AS in AT domain).

Depositor

James A. DeCaprio

Article

Borger et al J Virol. 2006 May . 80(9):4292-303.

Insert

EP300 (EP300 Human)

Use

Retroviral

Tags

Flag and HA

Mutation

partial (not complete) p300 fragment, AT and CH3 …

Available Since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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Opto-GPR176

Plasmid

#106060

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR176; Opto-GPR176; F2)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR176 (GPR176 Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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gh131

Plasmid

#106801

Purpose

expression of gRNA targeting CHPF

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

CHPF (CHPF Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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gh4

Plasmid

#106688

Purpose

expression of gRNA targeting GALNT4

Depositor

Eric-Paul Bennett

Article

Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

Insert

GALNT4 (GALNT4 Human)

Expression

Mammalian

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-mEKLF Zinc Fingers

Plasmid

#67837

Purpose

Expression of murine EKLF Zinc Fingers (aa 287-end) fused to Flag and driven by SV40 promoter

Depositor

James Bieker

Article

Chen et al Mol Cell Biol. 2001 May;21(9):3118-25.

Insert

EKLF (Klf1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Zinc Fingers are amino acids 287-end; this number…

Promoter

SV40

Available Since

Aug. 25, 2015

Availability

Academic Institutions and Nonprofits only
pBabe-CHES1-ER

Plasmid

#51846

Purpose

fusion protein between CHES1 and the ligand binding domain of estrogen receptor

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

ER

Available Since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES1H164A

Plasmid

#51844

Purpose

Flag-CHES1L mutant with ponctual mutation on histidine 164

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag

Available Since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES1L-dC

Plasmid

#51839

Purpose

Flag-CHES1L mutant lacking the C-terminal and NLS domains

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag

Mutation

contains CHES1 amino acids 1-205

Available Since

Aug. 4, 2014

Availability

Academic Institutions and Nonprofits only
pLPC-Flag-CHES1L-dN

Plasmid

#51843

Purpose

Flag-CHES1L mutant lacking the N-terminal domain

Depositor

Gerardo Ferbeyre

Article

Huot et al Mol Biol Cell. 2014 Mar;25(5):554-65. do

Insert

CHES1 (FOXN3 Human)

Use

Retroviral

Tags

Flag

Mutation

contains CHES1 amino acids 110-490

Available Since

July 17, 2014

Availability

Academic Institutions and Nonprofits only
HOXB6-WG-MSCV

Plasmid

#20993

Depositor

Corey Largman

Article

Shen et al J Biol Chem. 2004 Sep 17. 279(38):39895-

Insert

HOXB6 (HOXB6 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

W->G mutation in YPWM interaction motif for PB…

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
3691 pGEX4T-3 MLL AD mut.A

Plasmid

#8863

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Tags

GST

Expression

Bacterial

Mutation

ILPSDIMDFVL --> ILPSDIMDAAA

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
3692 pGEX4T-3 MLL AD mut.B

Plasmid

#8864

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Tags

GST

Expression

Bacterial

Mutation

ILPSDIMDFVL --> ILPSAAAAFVL

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only
3693 pGEX4T-3 MLL AD mut.C

Plasmid

#8865

Depositor

Stanley Korsmeyer

Article

Ernst et al Mol Cell Biol. 2001 Apr . 21(7):2249-58.

Insert

MLL min A.D. (KMT2A Human)

Tags

GST

Expression

Bacterial

Mutation

ILPSDIMDFVL --> ILPSNIMNFVL

Available Since

Aug. 3, 2005

Availability

Academic Institutions and Nonprofits only