We narrowed to 11,601 results for: 112

1. pcDNA3_SP-DRD2-V2R-NTEV-TCS-GV-2xHA

   Plasmid

   #194365

   Purpose

   Split TEV assays

   Depositor

   Michael Wehr

   Article

   Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

   Insert

   SP-DRD2-V2R-NTEV-TCS-GV-2xHA (DRD2 Synthetic, Human)
   

   Use

   Tags

   2xHA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. ATX3-JD

   Plasmid

   #184247

   Purpose

   Recombinant protein expression and purification. Encodes ataxin-3 Josephin domain fused with His-Tag (N-terminal)

   Depositor

   Sandra Macedo Ribeiro

   Article

   Figueiredo et al Cells. 2022 Jun 19;11(12). pii: cells111

   Insert

   Ataxin-3 Josephin domain (ATXN3 Human)
   

   Use

   Tags

   6x His-Tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Premature stop codon V183X

   Promoter

   T7 promoter

   Available since

   Sept. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGEX-2T-TRR-C421-G2304D

   Plasmid

   #182845

   Purpose

   mutation G2304D (GGC/GAC), PCR product coding C-terminal residues 2011-2431 of TRR was inserted into modified pGEX-2T by Nde I-Nsi I sites

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2014 Mar;141(5):1129-39. do

   Insert

   TRR 2011-2431 (trr Fly)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   mutation G2304D (GGC/GAC)

   Promoter

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGEX-2T-TRX-C751-E3616S

   Plasmid

   #182844

   Purpose

   mutation E3616S (GAA/TCA), PCR product coding C-terminal residues 2976-3726 of TRX was inserted into modified pGEX-2T by Nde I-Nsi I sites. Expression in E. coli. by IPTG induction

   Depositor

   Peter Harte

   Article

   Tie et al Development. 2014 Mar;141(5):1129-39. do

   Insert

   TRX (w Fly)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLV V5-Chpt1

   Plasmid

   #175155

   Purpose

   Lentiviral expression of V5-tagged mouse Chpt1

   Depositor

   Larry Gerace

   Article

   Cheng et al Life Sci Alliance. 2023 Jul 11;6(9):e202

   Insert

   Chpt1 (Chpt1 Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pIRESneo2 mycBioID-Tollip M240A,F241A

   Plasmid

   #178055

   Purpose

   myc-BioID fused to human Tollip carrying CUE domain mutations cloned into the pIRESneo2 plasmid. For generation of stable expressing mammalian cells used for proximity dependent biotin labelling.

   Depositor

   David Tumbarello

   Article

   Ryan et al EMBO J. 2020 Jun 2;39(11):e102539. doi:

   Insert

   Toll-Interacting Protein (TOLLIP Human)
   

   Use

   Tags

   mycBioID

   Expression

   Mammalian

   Mutation

   Changed Methionine 240 to Alanine and changed Phe…

   Promoter

   CMV

   Available since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. LentiGuide-Puro-NCOA7g3 (BB24)

   Plasmid

   #139459

   Purpose

   Lentiviral vector with gRNA targeting human NCOA7 short isoform; includes puromycin selectable marker

   Depositor

   Caroline Goujon

   Article

   Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

   Insert

   NCOA7 short isoform-targeting sgRNA inserted; resistance gene: puroR (NCOA7 Synthetic)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1a / U6

   Available since

   Oct. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. EF1a_KLF8_P2A_Hygro_Barcode

   Plasmid

   #120494

   Purpose

   Barcoded lentiviral vector to express KLF8 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   KLF8 (KLF8 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. Opto-GPR142

   Plasmid

   #106046

   Purpose

   Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR142; Opto-GPR142; D12)

   Depositor

   Harald Janovjak

   Article

   Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

   Insert

   Opto-GPR142 (GPR142 Human, Bovine)
   

   Use

   Tags

   Rhodopsin-1D4 and VSV-G

   Expression

   Mammalian

   Mutation

   Chimeric receptor protein

   Promoter

   CMV

   Available since

   Jan. 23, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. Opto-GPR45

    Plasmid

    #106023

    Purpose

    Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR45; Opto-GPR45; C1)

    Depositor

    Harald Janovjak

    Article

    Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

    Insert

    Opto-GPR45 (GPR45 Human, Bovine)
    

    Use

    Tags

    Rhodopsin-1D4 and VSV-G

    Expression

    Mammalian

    Mutation

    Chimeric receptor protein

    Promoter

    CMV

    Available since

    Jan. 23, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEGFP Kind2 S159/666E

    Plasmid

    #105313

    Purpose

    To study altered downstream functions of phospholylation-site mimetic kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159/666E

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEGFP KInd2 S159E

    Plasmid

    #105311

    Purpose

    To study altered downstream functions of phospholylation-site mimetic kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159E

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEGFP Kind2 S159/666A

    Plasmid

    #105309

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159/666A

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pEGFP Kind2 S666A

    Plasmid

    #105308

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S666A

    Promoter

    Available since

    Feb. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFP KInd2 S159A

    Plasmid

    #105306

    Purpose

    To study altered downstream functions of phospholylation-site null kindlin2

    Depositor

    Kenneth Yamada

    Article

    Artym et al J Cell Biol. 2015 Feb 2;208(3):331-50. d

    Insert

    Kindlin2 (FERMT2 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S159A

    Promoter

    Available since

    Feb. 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPN067

    Plasmid

    #91602

    Purpose

    Express sgRNA targeting human DPP4

    Depositor

    Lindy Barrett

    Article

    Hazelbaker et al Stem Cell Reports. 2017 Oct 10;9(4):1315

    Insert

    DPP4 (DPP4 Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Oct. 12, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. PNKP gRNA (BRDN0001149048)

    Plasmid

    #77163

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PNKP

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PNKP (PNKP Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. PNKP gRNA (BRDN0001144902)

    Plasmid

    #77165

    Purpose

    3rd generation lentiviral gRNA plasmid targeting human PNKP

    Depositor

    David Root, John Doench

    Article

    Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

    Insert

    PNKP (PNKP Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available since

    July 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. Lrrc4c-AP-His

    Plasmid

    #71959

    Purpose

    Expresses the extracellular region of the LRRC4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Lrrc4c (Lrrc4c Mouse)
    

    Use

    Tags

    AP-His

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. HINT2-bio-His

    Plasmid

    #53406

    Purpose

    Expresses full-length Histidine triad nucleotide-binding protein 2 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.

    Depositor

    Gavin Wright

    Article

    Sun et al Mol Cell Proteomics. 2015 Feb 23. pii: m

    Insert

    HINT2 (HINT2 Human)
    

    Use

    Tags

    His tag, enzymatic biotinylation sequence, and ra…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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