We narrowed to 15,149 results for: HAL
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Plasmid#42874Purposea single-wavelength red-shifted genetically encoded calcium indicator constructed from calmodulin and cp-mRubyDepositorInsertRed Fluorescent Calcium binding Protein
TagsEK (Enterokinase) Recognition Site, His Tag (6x),…ExpressionBacterialPromoterT7Available SinceFeb. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-FLEX-rc [Jaws-KGC-GFP-ER2]
Plasmid#78174PurposeAAV-mediated expression of Jaws-KGC-GFP-ER2 under the human synapsin promoter, in floxed/reversed (Cre-dependent) manner.DepositorInsertJaws-KGC-GFP-ER2
UseAAVTagsER2, GFP, and KGCExpressionMammalianMutationK200R W214FPromoterhuman synapsinAvailable SinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLH_Scr15
Plasmid#100537PurposeCEN/ARS plasmid with expression cassettes for PhyBNT-CreN and PIF3-NLS-CreC fusion proteins for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.DepositorUseCre/Lox and Synthetic BiologyTagsCreC, CreN, and SV40 NLSExpressionYeastMutationN-terminal version of PhyB (AA 1-621)/ Mutation o…PromoterFBA1 and TDH3Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRL_CT_GI_del1
Plasmid#98744PurposeFor red/far-red light-regulated gene expression in Saccharomyces cerevisiae. Integrates into the yeast ura3-52 locus. Encodes constitutively expressed PhyBNT-synTALE-DBD and PIF3-NLS-VP64AD fusions.DepositorUseSynthetic BiologyTagsSV40-NLS, VP64-AD, and synTALE-DBDExpressionYeastMutationmutated nucleotide 576 C to A, deleted amino acid…PromoterFBA1 and TDH3Available SinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-hHP1β
Plasmid#103829PurposeSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illuminationDepositorAvailable SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pOTTC1012 - pAAV EF1a DIO Nuc-eYFP
Plasmid#75082PurposeAn AAV packaging vector that expresses Cre-dependent nuclear-localized eYFP under control of the EF1a promoter.DepositorInsertNuc-eYFP
UseAAV and Cre/LoxTagsNLSExpressionMammalianPromoterEF1aAvailable SinceJan. 18, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCIBN-hTRF1-tagRFP-T
Plasmid#103811PurposeBLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitmentDepositorAvailable SinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLH_Scr16
Plasmid#100538PurposeCEN/ARS plasmid with PhyBNT-NLS-CreN and PIF3-NLS-CreC expression cassettes for red/far-red light-regulated Cre recombinase activity in Saccharomyces cerevisiae.DepositorUseCre/Lox and Synthetic BiologyTagsCdc1 NLS, CreC, CreN, and SV40 NLSExpressionYeastMutationAA 1-621 of PhyB/ Mutation nucleotide 576 C-->APromoterFBA1 and TDH3Available SinceJune 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEN-L1-K2-L2
Plasmid#80684PurposeGateway Entry vector containing a temperature-sensitive mouse DHFRDepositorUseSynthetic BiologyTags3xHAExpressionBacterial, Insect, Mammalia…Mutationmouse DHFR with N-terminal UbiquitinAvailable SinceAug. 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-hHP1β
Plasmid#103830PurposeSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illuminationDepositorAvailable SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISMRSA0482
Plasmid#96968Purposeexpression of methicillin-resistant Staphylococcus aureus orf 0482DepositorInsertMRSA ORF0482
TagsN-ter TEV protease cleavable 6HisExpressionBacterialMutationnonePromoterT7Available SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
CRY2low-tdTomato-Raf1
Plasmid#104176PurposeExpresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with tdTomato and Raf1DepositorExpressionMammalianMutationCRY2 aa 1-491 truncation, R489E A491DPromoterCMVAvailable SinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
CRY2low-mCherry-Raf1
Plasmid#104066PurposeExpresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherry and Raf1DepositorExpressionMammalianMutationCRY2 aa 1-491 truncation, R489E A491DPromoterCMVAvailable SinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pABD1275
Plasmid#68017PurposePIF1 protein overproduction for purification from bacterial cellsDepositorInsertPHY-INTERACTING FACTOR 1 (PIF1) (PIL5 AT2G20180, Mustard Weed)
Tags6x-HisExpressionBacterialMutationCodon Optimized for bacterial expression (GenScri…PromoterT7Available SinceDec. 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pEAHISMRSA2028
Plasmid#97001PurposeExpressing MRSA Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferaseDepositorInsertAsp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase
TagsN-ter TEV protease cleavable 6HisMutationnonePromoterT7Available SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISSOarD1
Plasmid#97067PurposeExpressing Sulfolobus solfataricus orf Sso Ard1 (N-terminal acetylase) proteinDepositorInsertS. solfataricus Sso Ard1 protein
TagsN-terminal TEV protease cleavable 6xHis tagMutationnonePromoterT7Available SinceDec. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEAHISSIRV131
Plasmid#97053PurposeExpressing Sulfolobus islandicus rudivirus (SIRV) orf 131 proteinDepositorInsertSIRV orf 131
TagsN-terminal TEV protease cleavable 6xHis tagExpressionBacterialMutationnonePromoterT7Available SinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-hGCN5mut
Plasmid#103828PurposeSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illuminationDepositorExpressionMammalianMutationhGCN5: A566G (E189G), G585T (K195N), G726A (silen…PromoterCMVAvailable SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-hGCN5-NLS
Plasmid#103827PurposeSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illuminationDepositorExpressionMammalianMutationhGCN5: A566G (E189G), G585T (K195N), G726A (silen…PromoterCMVAvailable SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPHR-EYFP-hGCN5
Plasmid#103826PurposeSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illuminationDepositorExpressionMammalianMutationhGCN5: A566G (E189G), G585T (K195N), G726A (silen…PromoterCMVAvailable SinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only