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PX458-SPNS1-sg2

Plasmid

#198567

Purpose

SPNS1-targeting sgRNA in PX458 vector

Depositor

Monther Abu-Remaileh

Article

Scharenberg et al Sci Adv. 2023 Apr 21;9(16):eadf8966. doi

Insert

SPNS1-sg2 (SPNS1 Human)

Use

CRISPR

Expression

Mammalian

Available Since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 W391A pRK5

Plasmid

#206084

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Tags

GFP

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA W391A pcDNA3

Plasmid

#206071

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCR1178

Plasmid

#111104

Purpose

Expresses FANCF_-_22647294.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCF gRNA (FANCF Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1224

Plasmid

#111105

Purpose

Expresses FANCF_-_22647359.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

FANCF gRNA (FANCF Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1118

Plasmid

#111110

Purpose

Expresses HELQ_+_84376961.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

HELQ gRNA (HELQ Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pCR1165

Plasmid

#111111

Purpose

Expresses HELQ_-_84376979.23-P1P2 sgRNA in pCR1068

Depositor

Jacob Corn

Article

Richardson et al Nat Genet. 2018 Aug;50(8):1132-1139. doi

Insert

HELQ gRNA (HELQ Human)

Use

Lentiviral

Available Since

Oct. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pUDE724

Plasmid

#103023

Purpose

expression of a Cpf1 programming crRNA targetting PDR12 (crPDR12-3S)

Depositor

Jean-Marc Daran

Article

Swiat et al Nucleic Acids Res. 2017 Dec 1;45(21):125

Insert

crPDR12-3.S (PDR12 Budding Yeast)

Use

CRISPR

Expression

Yeast

Promoter

SNR52

Available Since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pENTR-2B-Nkx2-5 Y191C

Plasmid

#98613

Purpose

Gateway entry vector for NKX2-5 Y191C

Depositor

Richard Harvey

Article

Bouveret et al Elife. 2015 Jul 6;4. doi: 10.7554/eLife.

Insert

NKX2-5 Y191C (Nkx2-5 Mouse)

Use

Entry vector

Mutation

Point mutation Y191C

Available Since

July 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-miR34a-P6

Plasmid

#64790

Purpose

Luciferase expression driven by miR-34a promoter P6

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P6 (MIR34A Human)

Use

Luciferase

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-miR34a-P3

Plasmid

#64787

Purpose

Luciferase expression driven by miR-34a promoter P3

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P3 (MIR34A Human)

Use

Luciferase

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-miR34a-P5

Plasmid

#64789

Purpose

Luciferase expression driven by miR-34a promoter P5

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P5 (MIR34A Human)

Use

Luciferase

Available Since

July 17, 2015

Availability

Academic Institutions and Nonprofits only

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pGL3-IRES-miR34a-P4

Plasmid

#64788

Purpose

Luciferase expression driven by miR-34a promoter P4

Depositor

Joshua Mendell

Article

Chang et al Mol Cell. 2007 Jun 8;26(5):745-52. Epub

Insert

miR-34a promoter P4 (MIR34A Human)

Use

Luciferase

Available Since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pBABE-puro-THAP11-rescue-3xFLAG

Plasmid

#36998

Depositor

Debu Chakravarti

Article

Parker et al Mol Cell Biol. 2012 May;32(9):1654-70. E

Insert

THAP11 (THAP11 Human)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Mutations were generated at codons 273-275 conver…

Available Since

May 21, 2012

Availability

Academic Institutions and Nonprofits only
dCas9-KRAB-MeCP2

Plasmid

#110821

Purpose

Improved dCas9 repressor-dCas9-KRAB-MeCP2

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB-MeCP2

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CMV

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pB-CAGGS-dCas9-KRAB

Plasmid

#110822

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CAGGS

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJT305 Pa01 Lenti pEF-attB LSR GFP

Plasmid

#184948

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

Pa01 Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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dCas9-KRAB

Plasmid

#110820

Purpose

Conventional dCas9 repressor-dCas9-KRAB

Depositor

Alejandro Chavez, George Church

Article

Yeo et al Nat Methods. 2018 Jul 16. pii: 10.1038/s

Insert

dCas9-KRAB

Expression

Mammalian

Mutation

Cas9 mutations to make dCas9=D10A+D839A+H840A+N86…

Promoter

CMV

Available Since

July 17, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-SNAP-Rab6a_T27N

Plasmid

#248794

Purpose

Expresses SNAP-Rab6a-T27N (dominant negative) in mammalian cells. Generated from Addgene #46782 plasmid.

Depositor

Michael Robichaux

Article

Hekmatara et al bioRxiv [Preprint]. 2025 Sep 25:2025.09.

Insert

Rab6a (RAB6A Human)

Tags

SNAPf

Expression

Mammalian

Mutation

Change T 27 to N

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pJT301 BxBi Lenti pEF-attB LSR GFP

Plasmid

#184944

Purpose

Lentiviral delivery of recombinase landing pad

Depositor

Michael Bassik, Ami Bhatt, Lacra Bintu, Patrick Hsu

Article

Durrant et al Nat Biotechnol. 2022 Oct 10. pii: 10.103

Insert

BxBi Lenti pEF1a-attB LSR GFP

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Oct. 11, 2022

Availability

Academic Institutions and Nonprofits only

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