We narrowed to 14,915 results for: pET

1. mouse KANK4 Ankyrin Repeats pET151

   Plasmid

   #164057

   Purpose

   Expresses mouse KANK4 Ank domain in bacterial cells

   Depositor

   Ben Goult

   Insert

   Kidney Ankyrin Repeat-Containing Protein 4 (Kank4 Mouse)
   

   Use

   Tags

   His-tag, TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Oct. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-Duet1-6xHis-TEV-NDP52

   Plasmid

   #190194

   Purpose

   Plasmid for the protein expression of 6xHis-TEV-NDP52 in a bacterial expression system. Internal reference: SMC401

   Depositor

   Sascha Martens

   Article

   Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   NDP52 (CALCOCO2 Human)
   

   Use

   Tags

   6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET_His6-MBP-TEV-Cas6-RT-Cas1_WT

   Plasmid

   #186274

   Purpose

   Expresses Thiomicrospira Cas6-RT-Cas1 in E. coli

   Depositor

   Jennifer Doudna

   Article

   Wang et al Nat Commun. 2021 May 6;12(1):2571. doi:

   Insert

   Thiomicropira Cas6-RT-Cas1
   

   Use

   Tags

   His6-MBP

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-Duet1-6xHis-TEV-OPTN

   Plasmid

   #190192

   Purpose

   Plasmid for the expression of 6xHis-TEV-OPTN. Internal reference: SMC397

   Depositor

   Sascha Martens

   Article

   Zaffagnini et al EMBO J. 2018 Mar 1;37(5). pii: embj.2017

   Insert

   optineurin (OPTN Human)
   

   Use

   Tags

   6xHis and TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET-His6-Sumo-CylK-Strep

   Plasmid

   #185962

   Purpose

   Expresses His6-Sumo-CylK-Strep in E. coli for refolding protein preparation

   Depositor

   Emily Balskus

   Article

   Braffman et al Elife. 2022 Feb 25;11. pii: 75761. doi:

   Insert

   CylK
   

   Use

   Tags

   His6-Sumo

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pETDuet1-LanCL2-H264A-C-Ter-His

   Plasmid

   #154187

   Purpose

   To express LanCL2-H264A in bacterial cells

   Depositor

   Wilfred van der Donk

   Article

   Lai et al Cell. 2021 Apr 27. pii: S0092-8674(21)00

   Insert

   LanCL2
   

   Use

   Tags

   Hexahistidine

   Expression

   Bacterial

   Mutation

   His264->Ala264

   Promoter

   Available since

   Aug. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pET Duet1 His6 SUMO D14

   Plasmid

   #177854

   Purpose

   Bacterial Expression of D14

   Depositor

   Karsten Melcher

   Article

   Zhao et al Cell Res. 2013 Mar;23(3):436-9. doi: 10.

   Insert

   D14
   

   Use

   Tags

   6xHis-SUMO

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pET24a His6 GST PYL2(14-188)

   Plasmid

   #177842

   Purpose

   Bacterial Expression of PYL2

   Depositor

   Karsten Melcher

   Article

   Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

   Insert

   PYR1-like 2 (PYL2 Mustard Weed)
   

   Use

   Tags

   6xHis-GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28a-Fgβ-anticalin-His-ybbr

   Plasmid

   #168040

   Purpose

   Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag, Fgβ peptide, and ybbr tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET15b p97-Strep-His E314Amb

    Plasmid

    #169021

    Purpose

    Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

    Depositor

    Hemmo Meyer

    Article

    Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

    Insert

    p97
    

    Use

    Tags

    His and SBP

    Expression

    Bacterial

    Mutation

    E314TAG

    Promoter

    T7

    Available since

    July 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET51b-His-TEV-SNAP-tag_dead_mt

    Plasmid

    #167270

    Purpose

    Overexpression of the dead variant (C145A) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAP-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    C145A

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET51b-His-TEV-SNAP-tag_fast_dead_mt

    Plasmid

    #167272

    Purpose

    Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli

    Depositor

    Kai Johnsson

    Article

    Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

    Insert

    SNAPf-tag
    

    Use

    Tags

    His x10

    Expression

    Bacterial

    Mutation

    E30R-C145A

    Promoter

    T7

    Available since

    April 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. CPH3567 (pET151_Spt6 1247-1451 R1282H)

    Plasmid

    #91816

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed arginine 1282 to histidine; deleted resid…

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CPH3495 (pET151_Spt6 1247-1451 K1435A)

    Plasmid

    #91817

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed lysine 1435 to alanine; deleted residues …

    Promoter

    T7

    Available since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pET-11a (GB1 WT - A34F/V39L)

    Plasmid

    #136610

    Purpose

    Expresses GB1 WT double mutant (A34F/V39L)

    Depositor

    Roberto Chica

    Article

    Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

    Insert

    GB1 WT double mutant (A34F/V39L)
    

    Use

    Tags

    6x-Histag

    Expression

    Bacterial

    Mutation

    Mutation from GB1 WT: A34F - V39L

    Promoter

    T7 promoter

    Available since

    Oct. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AAV-Synapsin-Ypet-3XNLS-WPRE

    Plasmid

    #159950

    Purpose

    nucleus marker

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    Ypet-3XNLS
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. AAV-Synapsin-Ypet-optoJNKi-WPRE

    Plasmid

    #159967

    Purpose

    optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    Ypet-optoJNKi
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET-45b-HOXA13(+7A)-IDR-mCherry

    Plasmid

    #145285

    Purpose

    Bacterial Expression of 6xHis HOXA13(+7A)IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13(+7A)-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    A-repeat expanded

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-45b-HOXA13(WT)-IDR-mCherry

    Plasmid

    #145284

    Purpose

    Bacterial Expression of 6xHis HOXA13(WT) IDR mCherry Fusion Protein

    Depositor

    Denes Hnisz

    Article

    Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

    Insert

    HOXA13(WT)-IDR (HOXA13 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET15-His-∆NGlpG(C104A/M247C)

    Plasmid

    #155133

    Purpose

    Expresses N-terminally His-tagged E. coli GlpG C104A/M247C variant (residues 87-276) from pET15b

    Depositor

    Sinisa Urban

    Article

    Cho et al Nat Struct Mol Biol. 2019 Oct;26(10):910

    Insert

    GlpG (glpG Escherichia coli)
    

    Use

    Tags

    His

    Expression

    Bacterial

    Mutation

    C104A/M247C, insert codes for GlpG residues 87-276

    Promoter

    T7

    Available since

    Sept. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge