We narrowed to 15,158 results for: pET

1. PET-28a-YTH domain of YTHDC1

   Plasmid

   #228778

   Purpose

   Protein expression plasmid for YTH domain of YTHDC1 in E.Coli.

   Depositor

   Huiqing Zhou

   Article

   Wang et al RSC Chem Biol. 2024 Jul 25;5(9):914-923.

   Insert

   YTH domain of YTHDC1 (YTHDC1 Human)
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Jan. 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET30-10His-FKBP(f36v)-SNAP

   Plasmid

   #200282

   Purpose

   For recombinant expression in E.coli (Rosetta) of a 10His-tag - FKBP(f36v) - SNAPtag fusion protein

   Depositor

   Kristina Ganzinger

   Article

   Niederauer et al Nat Commun. 2023 Jul 19;14(1):4345. doi:

   Insert

   FKBP-SNAPtag
   

   Tags

   His-tag

   Expression

   Bacterial

   Available Since

   Dec. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAM355 pET28a-GGG-Titin[V15P]-23-K-24-C-10

   Plasmid

   #226351

   Purpose

   Expression of Titin (V15P) substrate for 26S proteasome

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Expression

   Bacterial

   Mutation

   V15P

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAM356 pET28a-GGG-Titin[V15P]-23-K-29-C-5

   Plasmid

   #226352

   Purpose

   Expression of Titin (V15P) substrate for 26S proteasome

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Expression

   Bacterial

   Mutation

   V15P

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAM357 pET28a-GGG-Titin[V15P]-23-K-33-C-1

   Plasmid

   #226353

   Purpose

   Expression of titin (V15P) substrate for 26S proteasome

   Depositor

   Andreas Martin

   Article

   Jonsson et al Sci Adv. 2022 Dec 23;8(51):eadd9520. doi

   Insert

   titin (TTN Human)
   

   Expression

   Bacterial

   Mutation

   V15P

   Promoter

   T7

   Available Since

   Oct. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28a-SdrG-B2-ELP-HIS-ybbR

   Plasmid

   #169134

   Purpose

   E. coli expression of SdrG N2, N3 and B2 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
   

   Tags

   3xELP linker, 6x Histag, and ybbr tag

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pET28a-VHH-ddFLN4-HIS-ybbR

   Plasmid

   #169137

   Purpose

   E. coli expression of VHH domain containing ybbR tag and ddFLN4 fingerprint domain. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   VHH
   

   Tags

   6x His tag, ddFLN4 tag, and ybbr tag

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a- mCherry-FIVAR-HIS-ybbR

   Plasmid

   #169138

   Purpose

   E. coli expression of mCherry domain containing ybbR tag and FIVAR fingerprint domain. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   mCherry
   

   Tags

   6x Histag, FIVAR tag, and ybbr tag

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET28a-SdrG-B1-ELP-HIS-ybbR

   Plasmid

   #169133

   Purpose

   E. coli expression of SdrG N2, N3 and B1 domains containing ybbR tag and ELP linker. This construct was designed for AFM-SMFS measurements.

   Depositor

   Michael Nash

   Article

   Liu et al JACS Au. 2022 May 18;2(6):1417-1427. doi

   Insert

   Staphylococcus epidermidis fibrinogen-binding protein SD-repeat protein G
   

   Tags

   3xELP linker, 6x Histag, and ybbr tag

   Expression

   Bacterial

   Available Since

   Oct. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-U6-sgPet-1-hSyn-mCherry-KASH

    Plasmid

    #223227

    Purpose

    guideRNA targeting the mouse Pet-1 (Fev)

    Depositor

    Kyungjin Kim

    Article

    Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

    Insert

    Fev (Fev Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET-28b Salmonella NAT_6H C-terminal

    Plasmid

    #223436

    Purpose

    Salmonella NAT expression vector with C-terminal His6 tag

    Depositor

    Emily Balskus

    Article

    Mehta et al Nat Med. 2023 Mar;29(3):700-709. doi: 10

    Insert

    Salmonella NAT
    

    Tags

    His6

    Available Since

    Aug. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET21b-Cdc42/13C-FKBP-MBP

    Plasmid

    #214291

    Purpose

    Express split-Cdc42 fragment fused with FKBP and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-Cdc42 fragment fused with FKBP (CDC42 Human)
    

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Cdc42-Q61L

    Promoter

    T7

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET21b-Rac1/13C-FKBP-MBP

    Plasmid

    #214295

    Purpose

    Express split-Rac1 fragment fused with FKBP and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-Rac1 fragment fused with FKBP (RAC1 Human)
    

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    Rac1-Q61L

    Promoter

    T7

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET21b-RhoA/13C-FKBP-MBP

    Plasmid

    #214299

    Purpose

    Express split-RhoA fragment fused with FKBP and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-RhoA fragment fused with FKBP (RHOA Human)
    

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    RhoA-Q63L

    Promoter

    T7

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET21b-KRas/13C-FKBP-MBP

    Plasmid

    #214303

    Purpose

    Express split-KRas fragment fused with FKBP and MBP

    Depositor

    Cliff Stains

    Article

    He et al Angew Chem Int Ed Engl. 2024 Jul 26:e202

    Insert

    Split-KRas fragment fused with FKBP (KRAS Human)
    

    Tags

    Maltose-binding protein (MBP)

    Expression

    Bacterial

    Mutation

    KRas-Q61L

    Promoter

    T7

    Available Since

    July 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28_6xHis-GAL4-PARP1-CAT-L713F

    Plasmid

    #175949

    Purpose

    Bacterial expression of a fusion of yeast GAL4 DNA binding domain and human PARP1 CAT domain containing L713F gain-of-function mutation

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    GAL4 DNA binding domain (GAL4 Budding Yeast)
    

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    L713F, V762A for the 'PARP1-CAT-L713F' …

    Promoter

    T7

    Available Since

    July 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET21b-effector-ncRNA-rrt-His8

    Plasmid

    #194089

    Purpose

    Expresses Ec86 operon (effector, ncRNA and reverse transcriptase) in Escherichia coli

    Depositor

    Tingting Zou

    Article

    Wang et al Nat Microbiol. 2022 Sep;7(9):1480-1489.

    Insert

    Ec86 effector-ncRNA-rrt
    

    Tags

    His8

    Promoter

    T7

    Available Since

    May 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET28-H12TEV-hEIF5B-587~1220

    Plasmid

    #208637

    Purpose

    for E. coli expression truncated human eIF5B (ENST00000289371), aa587~1220

    Depositor

    Quanfu Ma

    Insert

    human eIF5B (ENST00000289371) aa 587~1220 (EIF5B Human)
    

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    contain SNP rs1376443, protein sequence not chang…

    Promoter

    T7

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pET28-RPL3-1~41-mEGFP(A206K)-TwStrep

    Plasmid

    #208628

    Purpose

    for E. coli expression of truncated human RPL3 (ENST00000216146), aa1~41, fused with mEGFP(A206K) and Twin Strep tag

    Depositor

    Quanfu Ma

    Insert

    human RPL3 (ENST00000216146) aa 1~41 (RPL3 Human)
    

    Tags

    6xHis, Twin Strep, and mEGFP(A206K)

    Expression

    Bacterial

    Mutation

    only aa 1~41, other residues not included

    Promoter

    T7

    Available Since

    May 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pET-29b(+)_His-CBD-EmEndoF3

    Plasmid

    #218194

    Purpose

    Bacterial expression of a His-tagged Chitin Binding fusion of Elizabethkingia miricola EndoF3

    Depositor

    Florence Pojer

    Insert

    EndoF3
    

    Tags

    tufA-His-CBD

    Expression

    Bacterial

    Mutation

    codon optimized Ecoli, residues 41-329

    Promoter

    T7

    Available Since

    April 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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