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pICH72400

Plasmid

#50338

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, Atug7 (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pLV.SP146.gp91.GP91.cHS4

Plasmid

#30480

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP146/gp91/GP91/cHS4

Use

Lentiviral

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pICH77911

Plasmid

#50342

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

3'UTR, polyadenylation signal/terminator, ags (A. tumefaciens)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pPtac

Plasmid

#166937

Purpose

Ptac single-level gene expression controller (SLC)

Depositor

Thomas Gorochowski

Article

Greco et al Nat Commun. 2021 Mar 19;12(1):1738. doi:

Insert

Genetic circuit expressing GFP- PTAC

Use

Synthetic Biology

Expression

Bacterial

Promoter

PTAC

Available Since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

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pICH41402

Plasmid

#50285

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

5'UTR, omega (Tobacco Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAMB_CAT-FspI

Plasmid

#61002

Purpose

T7-transcription template for CAT control mRNA used for in vitro translation

Depositor

Nils Walter

Article

Rinaldi et al Nat Commun. 2016 Jan 19;7:8976. doi: 10.

Insert

Chloramphenicol acetyltransferase

Use

Unspecified

Mutation

FspI site introduced after T7 terminator

Promoter

T7

Available Since

Jan. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pICH45125

Plasmid

#50261

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter, LHB1B1 (AT2g34430, A. thaliana)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 Antisense

Plasmid

#60632

Purpose

Negative Control for the pcDNA3.1(+) ZKSCAN1 Sense plasmid

Depositor

Jeremy Wilusz

Article

Liang et al Genes Dev. 2014 Oct 3. pii: gad.251926.1

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 20, 2015

Availability

Academic Institutions and Nonprofits only

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pICH49477

Plasmid

#50324

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, DsRed variant of RFP (Discosoma sp.)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICH41388

Plasmid

#50253

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

promoter (0.4 kb), 35s (Cauliflower Mosaic Virus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pUC57-Rluc-let-7-A114-N40

Plasmid

#50563

Purpose

Template DNA for in vitro transcription using T7 polymerase

Depositor

Yukihide Tomari

Article

Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

Insert

Luciferase-let7-A114-N40

Use

Cloning vector

Promoter

lac

Available Since

Feb. 6, 2014

Availability

Academic Institutions and Nonprofits only

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pBABE Rad S105N

Plasmid

#11214

Depositor

Ronald Kahn

Article

Zhu et al J Biol Chem. 1996 Jan 12. 271(2):768-73.

Insert

Rad (RRAD Human)

Use

Retroviral

Expression

Mammalian

Mutation

S105N

Available Since

March 1, 2006

Availability

Academic Institutions and Nonprofits only
pLV.CMVenh.gp91.GP91.cHS4

Plasmid

#30479

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

CMVenh/gp91/GP91/cHS4

Use

Lentiviral

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only

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pACSA_Pcpt_YFP

Plasmid

#82019

Purpose

pALM34; control for YFP expression from cyanobacterial phycocyanin truncated promoter

Depositor

Brian Pfleger

Article

Markley et al ACS Synth Biol. 2015 May 15;4(5):595-603

Insert

YFP

Promoter

Pcpt

Available Since

Sept. 13, 2016

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0029

Plasmid

#162311

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, strep tag, stop codon

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0KN0284

Plasmid

#162289

Purpose

Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for SP6-driven TNT SP6 Coupled Wheat Germ Extract System

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Type

Empty backbone

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEPQD0CM0028

Plasmid

#162310

Purpose

Level 0 C-terminal tag part for MoClo assembly, TTCG-GCTT

Depositor

Nicola Patron

Article

Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

Insert

C-terminal tag, 9 aa linker, strep tag, stop codon

Use

Synthetic Biology

Available Since

Jan. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pGEL088

Plasmid

#137896

Purpose

iSTU-CRISPR/Cas12a DR-DR structure crRNA insertion vector with OsCDPK2 intron

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGEL082

Plasmid

#137891

Purpose

iSTU-CRISPR/Cas9 with OsCDPK2intron, RZ structure backbone

Depositor

Yong Zhang

Article

Zhong et al Rice (N Y). 2020 Feb 3;13(1):8. doi: 10.

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Feb. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pRRL.SP107.gp91.eGFP

Plasmid

#30473

Depositor

Didier Trono

Article

Barde et al Gene Ther. 2011 Nov;18(11):1087-97. doi:

Insert

SP107/gp91/eGFP

Use

Lentiviral

Available Since

Aug. 24, 2011

Availability

Academic Institutions and Nonprofits only

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