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AttB_ACE2[HA]_IRES_iRFP670-H2A-P2A-PuroR

Plasmid

#190080

Purpose

express cytoplasmically HA-tagged human ACE2 with selectable markers iRFP670 and puromycin resistance gene

Depositor

Kenneth Matreyek

Article

Roelle et al PLoS Biol. 2022 Jul 27;20(7):e3001738. d

Insert

ACE2 (ACE2 Human)

Tags

HA

Expression

Mammalian

Promoter

none

Available Since

Sept. 8, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_113

Plasmid

#180540

Purpose

Entry vector containing Signal peptide region of CsgA including SecB-N22 sequenced

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Signal peptide region of CsgA including SecB-N22 sequenced

Expression

Bacterial

Available Since

Aug. 17, 2022

Availability

Academic Institutions and Nonprofits only

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p8322 pDONR ATG-YAP1 S94A-Stop

Plasmid

#184526

Purpose

Subcloning YAP1 S94A

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 S94A (YAP1 Human)

Use

Unspecified

Mutation

S94A

Available Since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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p8321 pDONR ATG-YAP1 S127A-Stop

Plasmid

#184525

Purpose

Subcloning YAP1 S127A

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

YAP1 S127A (YAP1 Human)

Use

Unspecified

Mutation

S127A

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_095

Plasmid

#180524

Purpose

dCas9 KTK compatible plasmid. Contains dCas9 and lacZ dropout region with flanking D1.1 overhangs for insertion of gRNA expression assembly

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Type

Empty backbone

Expression

Bacterial

Available Since

April 14, 2022

Availability

Academic Institutions and Nonprofits only

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KTK_324

Plasmid

#180566

Purpose

Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Spacer sequence in 2.2b format to allow for level 3 cloning with D2.2a

Expression

Bacterial

Available Since

April 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV(Δ3)-squirrel-monkey-full-length-CHMP3-HA

Plasmid

#154169

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV(Δ2)-squirrel-monkey-full-length-CHMP3-HA

Plasmid

#154170

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV(Δ1)-squirrel-monkey-full-length-CHMP3-HA

Plasmid

#154171

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pEF1α-squirrel-monkey-full-length-CHMP3-HA

Plasmid

#154172

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Tags

HA

Expression

Mammalian

Promoter

EF1-alpha

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-squirrel-monkey-CHMP3

Plasmid

#154182

Purpose

expresses squirrel monkey CHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

CHMP3

Tags

FLAG, Strep-Tag II, and preScission site

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCAG-OSF-PP-squirrel-monkey-retroCHMP3

Plasmid

#154184

Purpose

expresses squirrel monkey retroCHMP3 in mammalian cells

Depositor

Wesley Sundquist

Article

Rheinemann et al Cell. 2021 Sep 27. pii: S0092-8674(21)01

Insert

retroCHMP3

Tags

FLAG, Strep-Tag II, and preScission site

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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Gal4-TET1-878-911-I894A(pL84)

Plasmid

#124404

Purpose

Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with I894A mutation.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

Gal4-TET1-878-911(I894A mutation) (TET1 Human)

Tags

gal4 dna binding domain

Expression

Mammalian

Mutation

I894A

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-TET1-878-911-T898A(pL84)

Plasmid

#124403

Purpose

Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with T898A mutation.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

Gal4-TET1-878-911(T898A mutation) (TET1 Human)

Tags

gal4 dna binding domain

Expression

Mammalian

Mutation

T898A

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-TET1-878-911-L900A(pL84)

Plasmid

#124402

Purpose

Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L900A mutation.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

Gal4-TET1-878-911(L900A mutation) (TET1 Human)

Tags

gal4 dna binding domain

Expression

Mammalian

Mutation

L900A

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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Gal4-TET1-878-911-L897A(pL84)

Plasmid

#124401

Purpose

Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L897A mutation.

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

Gal4-TET1-878-911(L897A mutation) (TET1 Human)

Tags

gal4 dna binding domain

Expression

Mammalian

Mutation

L897A

Available Since

May 24, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-L900A(pL91)

Plasmid

#124395

Purpose

E.Coli produced TET1-SID mutants (L900A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-L900A (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

L900A

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-T898E(pL91)

Plasmid

#124394

Purpose

E.Coli produced TET1-SID mutants (T898E) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-T898E (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

T898E

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-T898A(pL91)

Plasmid

#124393

Purpose

E.Coli produced TET1-SID mutants (T898A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-T898A (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

T898A

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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GB1-His-TET1-878-911-L897A(pL91)

Plasmid

#124392

Purpose

E.Coli produced TET1-SID mutants (L897A) purified by FPLC nickel column

Depositor

Shaun Cowley

Article

Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

Insert

GB1-His-TET1-878-911-L897A (TET1 Human)

Tags

GB1-Histidine

Expression

Bacterial

Mutation

L897A

Available Since

May 23, 2019

Availability

Academic Institutions and Nonprofits only

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