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pCDNA5/FRT_CMV_NES-Caprola_01-mEGFP

Plasmid

#194681

Purpose

CMV driven expression of the calcium recorder Caprola_01 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_01-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_nLightR2

Plasmid

#221673

Purpose

Expresses nLightR2 in neurons

Depositor

Tommaso Patriarchi

Article

Rohner et al Nat Methods (2026). https://doi.org/10.1

Insert

nLightR2

Use

AAV

Expression

Mammalian

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pMitoTimer

Plasmid

#52659

Purpose

Expresses mitochondrial targeted DsRed1-E5 as reporter for mitochondrial content, structure, oxidative stress and damage in vitro and in vivo

Depositor

Zhen Yan

Article

Laker et al J Biol Chem. 2014 Apr 25;289(17):12005-1

Insert

DsRed1-E5

Use

Dsred

Tags

human cytochrome c oxidase subunit VIII mitochond…

Promoter

CMV

Available Since

April 22, 2014

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro_CMV_NES-Caprola_06-mEGFP

Plasmid

#194693

Purpose

CMV driven expression of the calcium recorder Caprola_06 fused to mEGFP for neuronal expression through through lentivirus transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_06-mEGFP

Use

Lentiviral

Tags

mEGFP

Promoter

CMV

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro_CMV_NES-Caprola_off-mEGFP

Plasmid

#194695

Purpose

CMV driven expression of the inactive calcium recorder negative control Caprola_off fused to mEGFP for neuronal expression through lentivirus transduction

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_off-mEGFP

Use

Lentiviral

Tags

mEGFP

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pEND-350_Cacnes_ori

Plasmid

#225605

Purpose

Cutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector optimized for reduced size and modular assembly, harbouring a medium-copy pBR322 E. coli ori (no ROP)

Depositor

Marc Güell

Article

Nevot et al Cell Syst. 2025 Feb 19;16(2):101169. doi

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_Cox8a(4x)-SNAP-tag2-T2A-EGFP

Plasmid

#226515

Purpose

Mamalian cell expression of SNAP-tag2 in the mitochondrial matrix and cytosolic EGFP

Depositor

Kai Johnsson

Article

Kühn et al Nat Chem Biol. 2025 Jul 3. doi: 10.1038/

Insert

Cox8a(4x)-SNAP-tag2-T2A-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT_CMV_NES-Caprola_09-mEGFP

Plasmid

#194685

Purpose

CMV driven expression of the calcium recorder Caprola_09 in mammalian cell lines fused to mEGFP

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_09-mEGFP

Tags

mEGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)-Caprola_15

Plasmid

#194676

Purpose

Protein production of the split HaloTag based calcium recorder Caprola_15 in bacteria

Depositor

Kai Johnsson

Article

Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

Insert

Caprola_15

Tags

His-tag and Strep-tag

Expression

Bacterial

Promoter

T7

Available Since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_TOMM20_HaloTag9

Plasmid

#169335

Purpose

Expression of HaloTag9 at the mitochondrial surface of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

TOMM20-HaloTag9 (TOMM20 Human)

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Available Since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-R-iLACCO1.2

Plasmid

#208027

Purpose

Biosensor for intracellular L-lactate

Depositor

Robert Campbell

Article

Nasu et al Nat Commun. 2023 Oct 27;14(1):6598. doi:

Insert

R-iLACCO1.2

Expression

Mammalian

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_hSyn_nLightG2

Plasmid

#221672

Purpose

Expresses nLightG2 in neurons

Depositor

Tommaso Patriarchi

Article

Rohner et al Nat Methods (2026). https://doi.org/10.1

Insert

nLightG2

Use

AAV

Expression

Mammalian

Available Since

Feb. 26, 2026

Availability

Academic Institutions and Nonprofits only

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pAAVS1-PC-GCaMP6f

Plasmid

#73503

Purpose

GCaMP6f knock in construct into the AAVS1 locus

Depositor

Bruce Conklin

Article

Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

Insert

GCaMP6f

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 7, 2016

Availability

Academic Institutions and Nonprofits only

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PACT deltaD3

Plasmid

#251700

Purpose

PRKRA (deletion of 197-313) protein expression construct in E. coli

Depositor

Sun Hur

Article

Ahmad et al Nat Commun. 2025 Apr 8;16(1):3325. doi:

Insert

PRKRA (PRKRA Human)

Expression

Bacterial

Mutation

deletion of 197-313

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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PACT deltaD2

Plasmid

#251699

Purpose

PRKRA (deletion of 110-198) protein expression construct in E. coli

Depositor

Sun Hur

Article

Ahmad et al Nat Commun. 2025 Apr 8;16(1):3325. doi:

Insert

PRKRA (PRKRA Human)

Expression

Bacterial

Mutation

deletion of 110-198

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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PACT wild type

Plasmid

#251697

Purpose

PRKRA protein expression construct in E. coli

Depositor

Sun Hur

Article

Ahmad et al Nat Commun. 2025 Apr 8;16(1):3325. doi:

Insert

PRKRA (PRKRA Human)

Expression

Bacterial

Available Since

March 27, 2026

Availability

Academic Institutions and Nonprofits only

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pT7II-0N4Rtau

Plasmid

#249555

Purpose

Recombinant protein synthesis of 0N4R isoform of human Tau

Depositor

Jeff Kuret

Article

Pettis et al Anal Biochem. 2026 Mar 4;714:116095. doi

Insert

Tau (0N4R) (MAPT )

Expression

Bacterial

Available Since

Jan. 14, 2026

Availability

Academic Institutions and Nonprofits only

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pWPI-SLC7A11-HA

Plasmid

#201643

Purpose

Over-expression of HA-tagged human SLC7A11

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

SLC7A11 (SLC7A11 Human)

Use

Lentiviral

Tags

HA

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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CROPseq-iT7

Plasmid

#211699

Purpose

NIS-Seq-compatible lentiviral vector for expressing sgRNAs

Depositor

Jonathan Schmid-Burgk

Article

Fandrey et al Nat Biotechnol. 2024 Dec 19. doi: 10.103

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLentiRNACRISPR_007 - TetO-NLS-RfxCas13d-NLS-WPRE-EFS-rtTA3-2A-Blast

Plasmid

#138149

Purpose

Expresses RfxCas13d in mammalian cells for knockdown of target RNAs in combination with compatible crRNAs. Localized to the nucleus

Depositor

Neville Sanjana

Article

Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.

Insert

CasRx

Use

Lentiviral

Tags

NLS-HA

Available Since

Feb. 20, 2020

Availability

Academic Institutions and Nonprofits only

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