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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,582 results for: eng

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Showing: 3901 - 3920 of 14582 results

  6. pCMV-PE5max-SF3B1-K700E

    Plasmid
    #200317
    Purpose
    Expresses the PE5max system to engineer the SF3B1 K700E mutation. Co-expresses both a puromycin resistance gene and tagRFP for selection.
    Depositor
    Lili Wang
    Publication
    Fernandez et al Cancer Res Commun. 2024 Aug 28. doi: 10.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    K700E-ng1+pg2
    PEmax-2A-MLH1dn
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    200317-plasmid-map-sequence-id-396311

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  7. pcDNA3-sACE2v2.4-sfGFP

    Plasmid
    #149666
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 2.4
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    May 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    149666-plasmid-map-sequence-id-298415

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  8. pcDNA3-sACE2v1-sfGFP

    Plasmid
    #145172
    Purpose
    Mammalian expression plasmid for sACE2-sfGFP high affinity variant 1
    Depositor
    Erik Procko
    Publication
    Chan et al Science. 2020 Sep 4;369(6508):1261-1265.
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Angiotensin-converting enzyme 2 (ACE2 Human)
    Tags
    Linker GSGGSGSGG and superfolder GFP
    Expression
    Mammalian
    Mutation
    Extracellular, soluble protease domain (a.a. M1-D…
    Promoter
    CMV
    Available since
    April 17, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    145172-plasmid-map-sequence-id-292576

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  9. pJOP-HTT-HR30Q

    Plasmid
    #92247
    Purpose
    HTT donor DNA with 30Q for homologous recombination-mediated gene modification
    Depositor
    Mahmoud Pouladi
    Publication
    Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HTT donor DNA (HTT Human)
    Use
    Precisionx hr targeting vector
    Mutation
    Barcode of wobble bases to prevent TALEN cleaving…
    Available since
    Feb. 26, 2019
    Availability
    Academic Institutions and Nonprofits only
    92247-plasmid-map-sequence-id-182400

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  10. pCAG-hdAsCas12a(D908A)-NLS(nucleoplasmin)-gs-3xHA-gs-VPR (RTW876)

    Plasmid
    #114078
    Purpose
    CAG promoter expression plasmid for human codon optimized AsCas12a-VPR(1.1)
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Publication
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a fused to VPR activation domain
    Tags
    SV40 NLS
    Expression
    Mammalian
    Available since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    114078-plasmid-map-sequence-id-220564

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  11. sgKRAS(B)

    Plasmid
    #85567
    Purpose
    Expresses sgRNA targeting human KRAS around codon G12
    Depositor
    Luca Grumolato
    Publication
    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRAS proto-oncogene, GTPase (KRAS Human)
    Expression
    Mammalian
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  12. sgKRAS(A)

    Plasmid
    #85566
    Purpose
    Expresses sgRNA targeting human KRAS around codon G12
    Depositor
    Luca Grumolato
    Publication
    Guernet et al Mol Cell. 2016 Aug 4;63(3):526-38. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    KRAS proto-oncogene, GTPase (KRAS Human)
    Expression
    Mammalian
    Available since
    Feb. 8, 2017
    Availability
    Academic Institutions and Nonprofits only

  14. pAAV-mDlx-NLS-mRuby2 (AAV2)

    Viral prep
    #99130-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-mDlx-NLS-mRuby2 (#99130). In addition to the viral particles, you will also receive purified pAAV-mDlx-NLS-mRuby2 plasmid DNA. mDlx-driven NLS-mRuby2 expression. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Viviana Gradinaru
    Publication
    Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.
    Promoter
    mDLX5/6
    Tags
    mRuby2
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only

  19. pAL-pyk_NT

    Plasmid
    #74072
    Purpose
    pAL374 plasmid carrying the pyk (NT) sgRNA targeting the non-template strand of pyk, SpecR
    Depositor
    Timothy Lu
    Publication
    Cleto et al ACS Synth Biol. 2016 Feb 16.
    Insert
    sgRNA pyk (NT)
    Use
    CRISPR
    Expression
    Bacterial
    Promoter
    ptac
    Available since
    April 12, 2016
    Availability
    Academic Institutions and Nonprofits only
    74072-plasmid-map-sequence-id-314169

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Showing: 3901 - 3920 of 14582 results