We narrowed to 32,680 results for: eng

1. pGP-CAG-Ace2N-4AA-mNeon-ST A122D-WPRE-bGH-polyA

   Plasmid

   #172912

   Purpose

   Mammalian expression of voltage sensor; soma localization targeting sequence

   Depositor

   GENIE Project

   Article

   Abdelfattah et al Neuron. 2023 Mar 28:S0896-6273(23)00205-

   Insert

   Ace2N-4AA-mNeon-ST A122D
   

   Tags

   soma localization targeting sequence

   Expression

   Mammalian

   Mutation

   A122D

   Promoter

   CAG

   Available Since

   Nov. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pHAGE-MAPK1-E322K

   Plasmid

   #116432

   Purpose

   Lentiviral expression of MAPK1 E322K

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   MAPK1 (MAPK1 Human)
   

   Use

   Lentiviral

   Mutation

   E322K

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. FUW-tetO-hOKMminiSOX

   Plasmid

   #206383

   Purpose

   Expresses four genes (human OCT4, KLF4, c-MYC and mouse miniSOX) in mammalian cells, for lentivirus generation.

   Depositor

   Ralf Jauch

   Article

   Hu et al Nucleic Acids Res. 2023 Aug 23:gkad597.

   Insert

   OCT4, KLF4, c-MYC, miniSOX (POU5F1, KLF4, MYC Mouse, Human)
   

   Use

   Lentiviral

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHybr_r2a>mG2b-srt-his

   Plasmid

   #124804

   Purpose

   HDR template to knock-in mouse IgG2b Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2b producing cell lines.

   Depositor

   Ferenc Scheeren, Martijn Verdoes

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   Murine IgG2b
   

   Use

   CRISPR; Hdr template

   Tags

   Histag (HHHHHH) and Sortag (LPETGG)

   Expression

   Bacterial and Mammalian

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHybr_r2a>mG2a-srt-his

   Plasmid

   #124803

   Purpose

   HDR template to knock-in mouse IgG2a Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG2a producing cell lines.

   Depositor

   Ferenc Scheeren, Martijn Verdoes

   Article

   van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

   Insert

   Murine IgG2a
   

   Use

   CRISPR; Hdr template

   Tags

   Histag (HHHHHH) and Sortag (LPETGG)

   Expression

   Bacterial and Mammalian

   Available Since

   Sept. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHAGE-KRAS-Q61R

   Plasmid

   #116425

   Purpose

   Lentiviral expression of KRAS Q61R

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   KRAS (KRAS Human)
   

   Use

   Lentiviral

   Mutation

   Q61R

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHAGE-PTPN11-E76Q

   Plasmid

   #116642

   Purpose

   Lentiviral expression of PTPN11 E76Q

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   PTPN11 (PTPN11 Human)
   

   Use

   Lentiviral

   Mutation

   E76Q

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE-ERBB2-G776V

   Plasmid

   #116333

   Purpose

   Lentiviral expression of ERBB2 G776V

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Mutation

   G776V

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-ERBB2-S310Y

   Plasmid

   #116350

   Purpose

   Lentiviral expression of ERBB2 S310Y

   Depositor

   Gordon Mills, Kenneth Scott

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   ERBB2 (ERBB2 Human)
   

   Use

   Lentiviral

   Mutation

   S310Y

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHybr_r2a>m2a(silent)-srt-his

    Plasmid

    #124807

    Purpose

    HDR template to knock-in FC silent mIgG2a isotype in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to FC silent producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Murine IgG2a (Fc silent)
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial and Mammalian

    Mutation

    L234A/L235A/N297A

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-Cry2-mCherry- YAP-C-IDR

    Plasmid

    #166447

    Purpose

    Expresses fusion of CRY2PHR with mCherry and YAP-C-IDR

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    Cry2, mCherry, YAP-C-IDR(266-504) (YAP1, CRY2 Human)
    

    Use

    Lentiviral

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. Lenti-Cry2-mCherry-TEAD4-IDR

    Plasmid

    #166449

    Purpose

    Expresses fusion of CRY2PHR with mCherry and TEAD4-IDR

    Depositor

    Shuguo Sun

    Article

    Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

    Insert

    Cry2, mCherry, TEAD4-IDR(89-230) (TEAD4, CRY2 Human)
    

    Use

    Lentiviral

    Available Since

    April 7, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHybr_r2a>mG1-srt-his

    Plasmid

    #124802

    Purpose

    HDR template to knock-in mouse IgG1 Fc domain in rat IgG2a heavy chain locus. Use in combination with PX458-gR2A_ISO to convert rat IgG2a hybridoma to mouse IgG1 producing cell lines.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Murine IgG1
    

    Use

    CRISPR; Hdr template

    Tags

    Histag (HHHHHH) and Sortag (LPETGG)

    Expression

    Bacterial and Mammalian

    Promoter

    -

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. PX459-gR2A_Hinge

    Plasmid

    #124811

    Purpose

    Vector for expression Cas9 with gRNA specific for rat IgG2a heavy chain locus. Use in combination with pHybr_r2a>Fab-srt-his plasmids to convert expression of hybridomas to Fab' fragments.

    Depositor

    Ferenc Scheeren, Martijn Verdoes

    Article

    van der Schoot et al Sci Adv. 2019 Aug 28;5(8):eaaw1822. doi:

    Insert

    Cas9 – gRNA_R2A_hinge
    

    Use

    CRISPR

    Tags

    3XFLAG and GFP

    Expression

    Bacterial and Mammalian

    Mutation

    R166H in PuroR (please see depositors comment bel…

    Promoter

    pUC

    Available Since

    Sept. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pHAGE-ERBB2-L755_T759delLRENT

    Plasmid

    #116339

    Purpose

    Lentiviral expression of ERBB2 L755_T759delLRENT

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Lentiviral

    Mutation

    L755_T759delLRENT

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pHAGE-KRAS-A146V

    Plasmid

    #116422

    Purpose

    Lentiviral expression of KRAS A146V

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    KRAS (KRAS Human)
    

    Use

    Lentiviral

    Mutation

    A146V

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHAGE-ERBB2-G776delinsVC

    Plasmid

    #116331

    Purpose

    Lentiviral expression of ERBB2 G776delinsVC

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Lentiviral

    Mutation

    G776delinsVC

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE-ERBB2-R678Q

    Plasmid

    #116347

    Purpose

    Lentiviral expression of ERBB2 R678Q

    Depositor

    Gordon Mills, Kenneth Scott

    Article

    Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

    Insert

    ERBB2 (ERBB2 Human)
    

    Use

    Lentiviral

    Mutation

    R678Q

    Available Since

    March 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPB-AMBRA1-501*-3xFLAG

    Plasmid

    #174160

    Purpose

    PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 501

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Missing aa 501-1208

    Promoter

    EF1A

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPB-AMBRA1-217*-3xFLAG

    Plasmid

    #174161

    Purpose

    PiggyBac vector to stably express 3xFLAG-tagged AMBRA1 truncated at amino acid 217

    Depositor

    Julien Sage

    Article

    Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

    Insert

    AMBRA1 (AMBRA1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Missing aa 217-1208

    Promoter

    EF1A

    Available Since

    Sept. 10, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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