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We narrowed to 155,767 results for: ins;

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Showing: 3901 - 3920 of 155767 results

  1. pLV-RnSyt1-sgRNA2-dCas9-KRAB-TagBFP2 (identifier AAAA-0246)

    Plasmid
    #202555
    Purpose
    Synaptotagmin-1 sgRNA2 with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCAGTACTCGCGTGCCTCGCACCGG) (Syt1 Rat)
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202555-plasmid-map-sequence-id-404691

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  2. pLV-RnSyt1-sgRNA3-dCas9-KRAB-TagBFP2 (Identifier AAAA-0247)

    Plasmid
    #202556
    Purpose
    Synaptotagmin-1 sgRNA3 with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ - CACCTCCTCCTGCAGCGGCAGCATCGG) (Syt1 Rat)
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202556-plasmid-map-sequence-id-404689

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  3. pLV-RnSyt1-sgRNA1-dCas9-KRAB-TagBFP2 (identifier AAAA-0245)

    Plasmid
    #202554
    Purpose
    Synaptotagmin-1 sgRNA1 with TagBFP2
    Depositor
    Frederic Meunier
    Article
    Joensuu et al EMBO J. 2023 Jul 3;42(13):e112095. doi:
    Insert
    CRISPRi single guide RNA sequence against 5’-UTR of rat synaptotagmin-1 (Syt1) gene (insert 5’ -CACCGCGTGCCTCGCACCGGTCCGCGG) (Syt1 R. norvegius (gRNA))
    Use
    CRISPR and Lentiviral
    Tags
    3xFlag (N-terminus of Cas9-KRAB), T2A-TagBFP2
    Available Since
    Dec. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    202554-plasmid-map-sequence-id-404687

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  8. ST-2xTstem(5A)-GFP

    Plasmid
    #162504
    Purpose
    Expresses a ST6GAL1 mutant with a GFP tag in its C-terminus in mammalian cells. One wildtype Tac stem region is inserted within ST and the other mutated Tac stem region is inserted between ST and GFP.
    Depositor
    Lei Lu
    Article
    Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014
    Insert
    GFP tagged ST6Gal1 mutant (ST6GAL1, IL2RA Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    One Tac stem region is inserted within ST6Gal1 an…
    Available Since
    Feb. 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    162504-plasmid-map-sequence-id-323646

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  9. pET-28b-T7-hAsCas12a-RVR(S542R/K548V/N552R)-NLS(nucleoplasmin)-6xHis (AAS1897)

    Plasmid
    #114074
    Purpose
    T7 promoter bacterial expression plasmid for human codon optimized AsCas12a-RVR(S542R/K548V/N552R) with C-terminal NLS and His-tag
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized AsCas12a-RVR (S542R/K548V/N552R) with C-terminal NLS and His-tag
    Tags
    NLS(nucleoplasmin) and 6xHis
    Expression
    Bacterial
    Mutation
    S542R, K548V and N552R
    Available Since
    Dec. 11, 2018
    Availability
    Academic Institutions and Nonprofits only
    114074-plasmid-map-sequence-id-220510

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  11. pCAG-denAsCas12a(E174R/S542R/K548R/D908A)-NLS(nuc)-3xHA-VPR (RTW776)

    Plasmid
    #107943
    Purpose
    Mammalian expression plasmid for human codon optimized DNase-inactive enAsCas12a (enhanced AsCas12a) fused to the VPR activation domain, encoding E174R/S542R/K548R/D908A substitutions
    Depositor
    Keith Joung, Benjamin Kleinstiver
    Article
    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103
    Insert
    human codon optimized DNase-inactive (D908A) enAsCas12a (E174R/S542R/K548R) fused to VPR activation domain
    Tags
    3x HA, NLS (nucleoplasmin), and VPR (VP64-p65-Rta…
    Expression
    Mammalian
    Mutation
    E174R, S542R, K548R and D908A
    Promoter
    CAG
    Available Since
    Feb. 11, 2019
    Availability
    Academic Institutions and Nonprofits only
    107943-plasmid-map-sequence-id-207614

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  12. pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting

    Plasmid
    #92139
    Purpose
    Targeting vector to introduce a Fucci cell cyle reporter cassette mCherry(G1)/BFP(S/G2) at the mouse TIGRE acceptor locus using puromycin selection
    Depositor
    Benoit Bruneau
    Article
    Nora et al Cell. 2017 May 18;169(5):930-944.e22. do
    Insert
    chromatin licensing and DNA replication factor 1 (GMNN, CDT1 Human)
    Use
    Mouse Targeting
    Expression
    Mammalian
    Available Since
    June 27, 2017
    Availability
    Academic Institutions and Nonprofits only
    92139-plasmid-map-sequence-id-349059

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  13. pRS314-Cef1-M175I

    Plasmid
    #234118
    Purpose
    Suppressor screen mutant in Cef2
    Depositor
    Aaron Hoskins
    Insert
    Cef1
    Expression
    Yeast
    Mutation
    M175I
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234118-plasmid-map-sequence-id-466510

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  14. pRS314-Cef1-Q193P

    Plasmid
    #234117
    Purpose
    Suppressor screen mutant in Cef1
    Depositor
    Aaron Hoskins
    Insert
    Cef1
    Expression
    Yeast
    Mutation
    Q193P
    Available Since
    March 25, 2025
    Availability
    Academic Institutions and Nonprofits only
    234117-plasmid-map-sequence-id-466508

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  15. pRS314-Cef1-A37V

    Plasmid
    #234119
    Purpose
    Suppressor screen mutant in Cef3
    Depositor
    Aaron Hoskins
    Insert
    Cef1
    Expression
    Yeast
    Mutation
    A37V
    Available Since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234119-plasmid-map-sequence-id-466463

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  16. pET283xFlagPP7CPHis

    Plasmid
    #28174
    Depositor
    Kathleen Collins
    Insert
    PP7 coat protein optimized
    Tags
    6xhistidine and triple FLAG peptide
    Expression
    Bacterial
    Mutation
    C68A,C73A
    Available Since
    Feb. 24, 2011
    Availability
    Academic Institutions and Nonprofits only
    28174-plasmid-map-sequence-id-13350

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  17. pRS414-Syf1

    Plasmid
    #234114
    Purpose
    Syf1 region PCR, restriction digest and ligation into pRS414 backbone
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Expression
    Yeast
    Available Since
    March 20, 2025
    Availability
    Academic Institutions and Nonprofits only
    234114-plasmid-map-sequence-id-465994

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  18. CMV500 A-ATF2

    Plasmid
    #33362
    Depositor
    Charles Vinson
    Insert
    ATF
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Dominant Negative (see comments)
    Promoter
    CMV
    Available Since
    Dec. 19, 2011
    Availability
    Academic Institutions and Nonprofits only
    33362-plasmid-map-sequence-id-152190

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  19. pAc5.1C-RLuc-A90-HhR_O

    Plasmid
    #147073
    Purpose
    Insect Expression of A90-HhR
    Depositor
    Elisa Izaurralde
    Insert
    A90-HhR
    Expression
    Insect
    Mutation
    A127 instead of A90 (see comments below)
    Available Since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    147073-plasmid-map-sequence-id-456973

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  20. pRS416-Syf1

    Plasmid
    #234113
    Purpose
    Syf1 region PCR, restriction digest and ligation into pRS416 backbone
    Depositor
    Aaron Hoskins
    Insert
    Syf1
    Expression
    Yeast
    Available Since
    March 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    234113-plasmid-map-sequence-id-466460

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Showing: 3901 - 3920 of 155767 results