We narrowed to 7,266 results for: 11

1. pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

   Plasmid

   #174899

   Purpose

   Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. ESR1-luciferase

   Plasmid

   #113351

   Purpose

   evaluate FOXA1 DIV motif enhancer activity near 50 kb of ESR1 gene

   Depositor

   Ralf Jauch

   Article

   Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

   Insert

   ESR1-enhancer (ESR1 Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pQHA-USP11 WT puroR

   Plasmid

   #46749

   Purpose

   Retroviral vector that expresses wild type HA-tagged wild type human USP11

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP11 (USP11 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. VRQR variant

   Plasmid

   #138562

   Purpose

   Expresses human codon-optimized SpCas9-VRQR and blasticidin resistance: EFS promoter-VRQR-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2020 Nov;38(11):1328-133

   Insert

   VRQR
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Mutation

   D1135V, G1218R, R1335Q, T1337R

   Promoter

   EFS

   Available since

   June 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pQHA-USP7 WT puroR

   Plasmid

   #46753

   Purpose

   Retroviral vector that expresses wild type HA-tagged human USP7

   Depositor

   Goedele Maertens, Gordon Peters

   Article

   Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

   Insert

   USP7 (USP7 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. 3xFlag-tdMCP-Erk2 (pErk reporter)

   Plasmid

   #226167

   Purpose

   Used to transiently express the pErk reporter in LABEL-seq MAPK activity assays.

   Depositor

   Dustin Maly

   Article

   Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

   Insert

   3xFlag-tdMCP-Erk2 (MAPK1 Human)
   

   Use

   Tags

   3xFlag-tdMCP

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. GB1-A11(89-196)

   Plasmid

   #196215

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(89-196).

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Use

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. GB1-A11(2-68)-Strep

   Plasmid

   #196216

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-68)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Use

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GB1-A11(2-52)-Strep

   Plasmid

   #196213

   Purpose

   Codon-optimized bacterial expression plasmid. Expresses GB1-His6-TEV-A11(2-52)-Strep.

   Depositor

   Lalit Deshmukh

   Article

   Shihora et al ACS Chem Neurosci. 2023 Jul 11. doi: 10.

   Insert

   Annexin A11 (ANXA11 Human)
   

   Use

   Tags

   GB1-6xHis-TEV and Strep-II tag

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

    Plasmid

    #140018

    Purpose

    Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    EcoTyrRS
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y37L, D182S, F183M, L186A

    Promoter

    EF1

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pQHA-USP11 CS puroR

    Plasmid

    #46750

    Purpose

    Retroviral vector that expresses catalytically inactive form of HA-tagged human USP11

    Depositor

    Goedele Maertens, Gordon Peters

    Article

    Maertens et al EMBO J. 2010 Aug 4;29(15):2553-65. doi:

    Insert

    USP11 (USP11 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    C318S--catalytically inactive

    Promoter

    CMV

    Available since

    March 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAS_4xMma PylT_FLAG-AcKRS

    Plasmid

    #140021

    Purpose

    Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    AcKRS-3 (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    L301M, L305I, Y306F, L309A, C348F

    Promoter

    EF1

    Available since

    May 20, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. MLF2-GFP-3xFLAG

    Plasmid

    #230986

    Purpose

    Expresses GFP tagged MLF2 in mammalian cells

    Depositor

    Christian Schlieker

    Article

    Prophet et al Nat Cell Biol. 2022 Nov;24(11):1630-1641

    Insert

    MLF2 (MLF2 Human)
    

    Use

    Tags

    3xFLAG and GFP

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    Jan. 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pKD068

    Plasmid

    #136471

    Purpose

    SSB-GFP IDL fusion expression plasmid

    Depositor

    James Keck

    Article

    Dubiel et al Nucleic Acids Res. 2020 Jun 19;48(11):60

    Insert

    SSB-GFP (ssb E. coli)
    

    Use

    Tags

    superfolder GFP

    Expression

    Bacterial

    Mutation

    superfolder GFP inserted between Phe148 and Ser149

    Promoter

    T7 promoter

    Available since

    April 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pGL4 mIL-17 2kb RORgamma-t mut

    Plasmid

    #20127

    Depositor

    Warren Strober

    Article

    Fuping et al 2008 Nov;9(11):1297-306. Epub 2008 Oct 1

    Insert

    mIL-17 (Il17a Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    RORgamma-t binding site in 0.9kb of 2kb promoter …

    Promoter

    Available since

    Jan. 16, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pCfB2904(XI-3 MarkerFree)

    Plasmid

    #73276

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XI-3 (Chr XI: 93378..94567)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Cx30-msfGFP

    Plasmid

    #69019

    Purpose

    Expresses human Cx30 (GJB6 CDS) with a 7 amino acid linker on the C-terminus of the Connexin linking to monomerized superfolder Green Fluorescent Protein. Expression driven by CMV promoter.

    Depositor

    David Spray

    Article

    Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

    Insert

    Cx30 (GJB6 Human)
    

    Use

    Tags

    V206K monomerized super folder GFP

    Expression

    Mammalian

    Mutation

    Promoter

    c

    Available since

    Oct. 23, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pEGFP-MyosinVa-Ctail

    Plasmid

    #110169

    Purpose

    Dominant negative effect by competing with MyosinVa C-terminal tail interactions

    Depositor

    Jose Esteban

    Article

    Correia et al Nat Neurosci. 2008 Apr;11(4):457-66. doi

    Insert

    Myosin Va (Myo5a Mouse)
    

    Use

    Tags

    EGFP and Myc

    Expression

    Mammalian

    Mutation

    amino acids 1444-1853

    Promoter

    CMV

    Available since

    May 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pB-CAG-FLEX-F14F15-BFP-(mCherry)'

    Plasmid

    #201831

    Purpose

    piggyBac transposon plasmid for making Cre and Flpo reporter cell lines using piggyBac transposase

    Depositor

    Ian Wickersham

    Article

    Jin et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Inserts

    CAG
    BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    April 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCfB2909(XII-5 MarkerFree)

    Plasmid

    #73281

    Purpose

    EasyClone-MarkerFree backbone vector for the addition of 1 or 2 genes and promoters for integration into site XII-5 (Chr XII: 839226..840357)

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge