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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4,732 results for: bre

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Showing: 3921 - 3940 of 4732 results

  2. pMJ1.19 (AAVS1 RNA donor in pCDNA3.1)

    Plasmid
    #238007
    Purpose
    300bp donor RNA complementary to Cas9 cut site at AAVS1 (Addgene plasmid #72833) with intronic sequence intervening the 3bp insertion signature (ATC). Can be used to measure RT-DSBR in human cells.
    Depositor
    Simon Powell, Agnel Sfeir
    Article
    Jalan et al Nat Commun. 2025 May 10;16(1):4349. doi:
    Insert
    AAVS1 (AAVS1, PPP1R12C Human)
    Use
    Rna donor to study rt-dsbr
    Expression
    Mammalian
    Mutation
    3bp insertion signature (ATC) at Cas9 cut site in…
    Promoter
    CMV
    Available Since
    May 14, 2025
    Availability
    Academic Institutions and Nonprofits only
    238007-plasmid-map-sequence-id-472649

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  3. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(D256A/PAMmut)

    Plasmid
    #176141
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutation Asp256Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resistance cassette
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Myc-tagged PolB with mutation in Asp256 to Ala, a…
    Promoter
    EF1A
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176141-plasmid-map-sequence-id-353041

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  4. pLV-Hygro-EF1a-XRCC1-EGFP-T2A-Myc-POLB(K35A/K68A/K72/PAMmut)

    Plasmid
    #176140
    Purpose
    EGFP fused to the C-terminus of XRCC1, linked by T2A to N-terminus Myc-tagged POLB with the mutations Lys35Ala, Lys68Ala, Lys72Ala, a mutation in the PAM site used by POLBKO gRNA1 & a hygromycin resis
    Depositor
    Robert Sobol
    Article
    Koczor et al Cell Rep. 2021 Nov 2;37(5):109917. doi:
    Insert
    XRCC1-T2A-PolB (XRCC1, POLB Human)
    Use
    Lentiviral
    Tags
    EGFP and MYC
    Expression
    Mammalian
    Mutation
    Myc-tagged PolB with mutations Lys35 to Ala, Lys6…
    Promoter
    EF1A
    Available Since
    Jan. 26, 2022
    Availability
    Academic Institutions and Nonprofits only
    176140-plasmid-map-sequence-id-353035

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  5. pUAP1

    Plasmid
    #63674
    Purpose
    Accepts DNA sequences via BpiI cloning sites, resulting in Level 0 standard parts for Golden Gate cloning. Parts can be released with a user-defined 4-base-pair, 5 prime overhangs using BsaI.
    Depositor
    Nicola Patron
    Article
    Patron et al New Phytol. 2015 Jul 14. doi: 10.1111/np
    Insert
    RFP cloning selection cassette
    Use
    Synthetic Biology
    Available Since
    April 24, 2015
    Availability
    Academic Institutions and Nonprofits only
    63674-plasmid-map-sequence-id-106088

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  12. mCherry-Ykt6

    Plasmid
    #209888
    Purpose
    Expresses mCherry tagged Ykt6 in mammalian cells
    Depositor
    Geert van den Bogaart
    Insert
    Ykt6
    Tags
    mCherry
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Nov. 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    209888-plasmid-map-sequence-id-413665

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  14. SDC4A-c001

    Plasmid
    #205119
    Purpose
    SDC4 protein expression
    Depositor
    Paul Brennan
    Insert
    SDC4 (SDC4 Human)
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    205119-plasmid-map-sequence-id-411015

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  15. QPRTA-c002

    Plasmid
    #205115
    Purpose
    QPRT protein expression
    Depositor
    Paul Brennan
    Insert
    QPRT (QPRT Human)
    Tags
    TEV-6His
    Expression
    Insect
    Mutation
    WT
    Available Since
    Oct. 13, 2023
    Availability
    Academic Institutions and Nonprofits only
    205115-plasmid-map-sequence-id-410409

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  16. pOpen-HSlig4

    Plasmid
    #165503
    Purpose
    Ligase used in efficiently joining single-strand breaks in a double-strand polydeoxynucleotide in an ATP-dependent reaction.
    Depositor
    Drew Endy, Jennifer Molloy, FreeGenes Project
    Insert
    DNA Ligase IV
    Use
    Synthetic Biology
    Available Since
    July 27, 2021
    Availability
    Industry, Academic Institutions, and Nonprofits
    165503-plasmid-map-sequence-id-338457

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  17. SLIT2A-c003

    Plasmid
    #205125
    Purpose
    SLIT2 protein expression
    Depositor
    Paul Brennan
    Insert
    SLIT2 (SLIT2 Human)
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    205125-plasmid-map-sequence-id-412271

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  18. SLIT1A-c003

    Plasmid
    #205124
    Purpose
    SLIT1 protein expression
    Depositor
    Paul Brennan
    Insert
    SLIT1 (SLIT1 Human)
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Oct. 25, 2023
    Availability
    Academic Institutions and Nonprofits only
    205124-plasmid-map-sequence-id-412475

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  19. SDCBPA-c023

    Plasmid
    #205121
    Purpose
    SDCBP protein expression
    Depositor
    Paul Brennan
    Insert
    SDCBP (SDCBP Human)
    Tags
    6His
    Expression
    Bacterial
    Mutation
    WT
    Available Since
    Oct. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    205121-plasmid-map-sequence-id-411056

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  20. SFRP1A-c500

    Plasmid
    #205122
    Purpose
    SFRP1 protein expression
    Depositor
    Paul Brennan
    Insert
    SFRP1 (SFRP1 Human)
    Tags
    AviTag-6His and PTPRSsp
    Expression
    Mammalian
    Mutation
    WT
    Available Since
    Dec. 22, 2023
    Availability
    Academic Institutions and Nonprofits only
    205122-plasmid-map-sequence-id-412368

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Showing: 3921 - 3940 of 4732 results