We narrowed to 28,194 results for: sta
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Plasmid#128398PurposePeriplasmic expression of E. coli MBP with C terminal 4xDQNAT glycosylation sites in E. coliDepositorInsertMaltose binding protein
Tags4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…ExpressionBacterialMutationY342H- please see depositor commentsPromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
JF001A
Plasmid#53116Purposeexpresses alpha hemolysin under the control of theophylline riboswitchDepositorInserttheophylline riboswitch and alpha hemolysin
ExpressionBacterialPromoterT7 promoterAvailable SinceMay 19, 2014AvailabilityAcademic Institutions and Nonprofits only -
MTOR-F2108L_CD19-CAR-28z-2A-EGFP_AAV6_Donor
Plasmid#211904PurposeVector for AAV6 donor production. Targeted CD19-CAR-28z-2A-EGFP transgene integration to the MTOR locus with the dominant rapamycin resistance MTOR-F2108L mutation via homology-directed repair.DepositorInsertCD19-CAR-28z-2A-EGFP
UseAAVExpressionMammalianPromoterhPGK1Available SinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pQE80L-SH3
Plasmid#160331PurposeThis plasmid encodes an engineered oxygen-independent flavin binding fluorescent protein with N-terminal 6xHis tag. Its thermostability and quantum yield is improved compared to wild type.DepositorInsertSH3
Tags6xHis tagExpressionBacterialPromoterT5Available SinceMarch 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
VEGF binding scFv G6
Plasmid#111716PurposeYeast Surface Display of G6 as a reference starting point for designDepositorInsertG6
Tagscmyc tagExpressionYeastAvailable SinceJuly 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNAintron-CHIKV-nsp3-c3xFLAG
Plasmid#215696PurposeProduces the Chikungunya virus nonstructural protein 3 with a c-terminal 3xFLAG tagDepositorInsertCHIKV nsp3
Tags3xFLAGExpressionMammalianPromoterCMVAvailable SinceAug. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLVX-mCherry-SPASTIN
Plasmid#180647PurposeLentiviral expression vector for SPASTIN. Used for generating cell lines. Has N-terminal mCherry tag. SPASTIN starts on M87. Dox-inducible. Internal ID: WISP20-43.DepositorInsertSPASTIN
UseLentiviralTagsmCherryExpressionMammalianMutationStarts at M87, has siRNA resistance to GAACAGUGUG…Available SinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDIRECT_26H
Plasmid#91150PurposeDirect Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:barDepositorInsertEngineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
UseCRISPRExpressionPlantAvailable SinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only -
pK1E-RNAP
Plasmid#48144PurposeShuttle vector E. coli/B.meg.; encoding the RNA polymerase of the phage K1E under control of the xylose inducible promoter PxylADepositorInsertrna polymerase K1E
UseSynthetic BiologyExpressionBacterialAvailable SinceOct. 21, 2013AvailabilityAcademic Institutions and Nonprofits only -
HOXB8 (murine) HIS-tag pET
Plasmid#8540DepositorInsertHOXB8 (Hoxb8 Mouse)
TagsHis and T7ExpressionBacterialMutationstarts at amino acid #2 (Ser)Available SinceAug. 7, 2006AvailabilityAcademic Institutions and Nonprofits only -
kiCAP-AAV-PAL1C
Plasmid#196685PurposeRep/Cap plasmid for the production of PAL1C, an AAV capsid with CNS tropism in macaques.DepositorInsertAAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
UseAAVMutationPTQGTLR insert between amino acids 588 and 589 of…Promoterp41Available SinceApril 7, 2023AvailabilityAcademic Institutions and Nonprofits only -
hCCR5-LSSmOrange
Plasmid#110194PurposeEncodes for human CCR5 coding sequence tagged with the LSS-mOrange FPDepositorAvailable SinceMay 15, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-Ha-Brca1 S1387A
Plasmid#32532DepositorInsertBrca1 (BRCA1 Human)
TagsHAExpressionMammalianMutationS1387APromoterCMV/Rabbit B-GlobinAvailable SinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE
Plasmid#123360PurposeLentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance.DepositorInsertEmpty LbCpf1 crRNA cloning cassette
UseCRISPR and LentiviralExpressionMammalianPromoterU6Available SinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrc99S-ssDsbA-EPA-DNNNS-DQNRT
Plasmid#128404PurposePeriplasmic expression of P. aeruginosa exotoxin A with DNNNS and DQNRT internal glycosylation sites in E. coliDepositorInsertExotoxin A (toxA P. aeruginosa)
Tags6xHis tag and DsbA signal sequence for periplasmi…ExpressionBacterialMutation242DNNNS246 and 384DQNRT388 engineered glycosylat…PromotertrcAvailable SinceApril 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
iRFP713-N2-Frankenbody-Flag
Plasmid#175291PurposeVisualize endogeneous loci coupled with ZF probes harboring 10xFLAG tagDepositorInsertanti-FLAG frankenbody fused with iRFP713
TagsiRFP713ExpressionMammalianAvailable SinceDec. 3, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCDH-EFS-FlpO
Plasmid#191509PurposeLentiviral Flp Recombinase driven by the EFS promoterDepositorInsertflp recombinase (flp )
UseLentiviralExpressionMammalianPromoterEF1a/EFS promoter insertedAvailable SinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMP71-mIL15-IL12rb1-sushi-il12rb2
Plasmid#174606PurposeExpresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tagsDepositorInsertExpresses a constitutively activated murine IL-12 receptor tagged with extracellular myc and flag tags
ExpressionMammalianAvailable SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pENTR1A DHFR.cHSF1
Plasmid#134737PurposeGateway entry vector for constitutively active HSF1 fused to the DHFR destabilized domainDepositorInsertDHFR.cHSF1 (HSF1 Human)
UseEntry vector for gateway cloningTagsDHFRMutationDeletion of amino acids 186-202PromoterNoneAvailable SinceOct. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
MSP2253
Plasmid#70704PurposeBacterial expression plasmid for KKH SaCas9 & sgRNA targeted to site 1: T7-humanSaCas9(E782K/N968K/R1015H)-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized KKH variant Staphylococcus aureus Cas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationE782K, N968K and R1015H in SaCas9PromoterT7Available SinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only