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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,476 results for: alp

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Showing: 3941 - 3960 of 14476 results

  1. pCAG CAPN7 V18D,F98D-OSF

    Plasmid
    #193045
    Purpose
    Mammalian expression plasmid encoding full-length CAPN7 with V18D and F98D mutations that diminishes binding to IST1. Contains C-terminal OSF tag (One-Strep-FLAG)
    Depositor
    Wesley Sundquist
    Article
    Paine et al Elife. 2023 Sep 29;12:e84515. doi: 10.75
    Insert
    CAPN7 (CAPN7 Human)
    Tags
    OSF (One-Strep-FLAG)
    Expression
    Mammalian
    Mutation
    V18D, F98D
    Promoter
    Chicken Beta-actin
    Available Since
    Dec. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    193045-plasmid-map-sequence-id-378994

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  5. pEcRNAP6

    Plasmid
    #128940
    Purpose
    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoA, B, C-ppx(His)10, Z
    Depositor
    Seth Darst
    Article
    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:
    Inserts
    RpoA (rpoA E. coli)
    RpoB
    RpoC-ppx(His)10
    RpoZ
    Tags
    His
    Expression
    Bacterial
    Mutation
    PreScission protease site (GE Healthcare; LEVLFQG…
    Promoter
    T7
    Available Since
    Aug. 29, 2019
    Availability
    Academic Institutions and Nonprofits only
    128940-plasmid-map-sequence-id-254597

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  9. pCMV-αIFNα-ab

    Plasmid
    #110799
    Purpose
    Expression and secretion of an scFv antibody (clone) 4EA1 against all mouse interferon α subtypes in mammalian cells
    Depositor
    Thomas Böldicke
    Article
    Bussow et al PLoS One. 2019 Apr 16;14(4):e0215062. do
    Insert
    antibody against mouse interferon alpha
    Tags
    ER signal peptide, His6 tag, and myc epitope tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    110799-plasmid-map-sequence-id-211397

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  10. pCMV-αIFNα-ib

    Plasmid
    #110798
    Purpose
    Expression of an intrabody derived from the monoclonal antibody 4EA1 against all mouse interferon α subtypes in mammalian cells
    Depositor
    Thomas Böldicke
    Article
    Bussow et al PLoS One. 2019 Apr 16;14(4):e0215062. do
    Insert
    intrabody against mouse interferon alpha
    Tags
    ER retention signal (SEKDEL), ER signal peptide, …
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 16, 2019
    Availability
    Academic Institutions and Nonprofits only
    110798-plasmid-map-sequence-id-211394

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  14. pEF1a_HH-EMX1 spacer-gRNA scaffold-EC73-5'-donor template-EC73-3'-HDV_pA_pCMV_EGFP-L138S-P2A-mCherry_pA

    Plasmid
    #176459
    Purpose
    Vector for expressing retron Ec73ncRNA-gRNA chimeric RNA (rgRNA) targeting EMX1 driven by EF1alpha promoter and and EGFP(L138S)-P2A-mCherry driven by CMV
    Depositor
    Hui Yang
    Article
    Kong et al Protein Cell. 2021 Aug 17. pii: 10.1007/
    Insert
    HH-EMX1 spacer-gRNA scaffold-Ec73ncRNA-donor sequence-HDV
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    EF1alpha
    Available Since
    Oct. 26, 2021
    Availability
    Academic Institutions and Nonprofits only
    176459-plasmid-map-sequence-id-346304

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Showing: 3941 - 3960 of 14476 results