We narrowed to 17,501 results for: puro

1. SaLgCP

   Plasmid

   #164562

   Purpose

   Puro

   Depositor

   Junwei Shi

   Article

   Verma et al Nat Cell Biol. 2021 Jan 18. pii: 10.1038

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Flag-NLS-SaCas9-P2A-PuroR

   Expression

   Mammalian

   Mutation

   Promoter

   U6 promoter for crRNA expression and EFS promoter…

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. MSCV-PIG-FLAG-TAF4(827-909)

   Plasmid

   #105617

   Purpose

   retrovirally express mouse TAF4 HFD with 3*FLAG at N terminal, GFP marker and puro resistance,

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   FLAG tagged TAF4 HFD-827-909 (Taf4 Mouse)
   

   Use

   Retroviral

   Tags

   3*FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   MSCV-LTR

   Available since

   Feb. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB9P2

   Plasmid

   #210030

   Purpose

   Contains PE2 prime editing gRNA cloning site, inducible prime editor 2, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   prime editor 2
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α and TRE3G

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPB9P3

   Plasmid

   #210034

   Purpose

   Contains cloning site for PE3/3b prime editing gRNA constructs, inducible prime editor 2, rtTA-T2A-puro

   Depositor

   Michael Talkowski

   Article

   Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

   Inserts

   prime editor 2
   rtta-T2A-puro
   

   Use

   CRISPR

   Tags

   SV40 NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF-1α and TRE3G

   Available since

   Feb. 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-Creb5(HA)

   Plasmid

   #195024

   Purpose

   HA-tagged Creb5 in pLenti CMV Puro DEST (w118-1) vector

   Depositor

   Andrew Lassar, Cheng-Hai Zhang

   Article

   Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

   Insert

   HA-tagged Creb5 (full length, 508aa) (CREB5 Bovine)
   

   Use

   Lentiviral

   Tags

   3xHA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCV-PIG-N3FLAG-TAF12

   Plasmid

   #105593

   Purpose

   retrovirally express mouse TAF12 with 3*FLAG tag at N terminal, GFP marker and puro resistance

   Depositor

   Christopher Vakoc

   Article

   Xu et al Cancer Cell. 2018 Jan 8;33(1):13-28.e8.

   Insert

   full length TAF12 with silent mutations, making it resistant to TAF12 shRNA#364 (Taf12 Mouse)
   

   Use

   Retroviral

   Tags

   3*FLAG

   Expression

   Mammalian

   Mutation

   silent mutations to make it resistant to the targ…

   Promoter

   MSCV-LTR

   Available since

   May 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLentiV2-dCas9-VP64

   Plasmid

   #141104

   Purpose

   Expression of gRNA and dCas9-VP64 from the same plasmid for CRISPRa

   Depositor

   Igor Ulitsky

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV CMV FST-HA

   Plasmid

   #175168

   Purpose

   Lentiviral expression of human FST-HA

   Depositor

   Larry Gerace

   Insert

   FST
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO-sh-BNIP3-FL

   Plasmid

   #100771

   Purpose

   Lentiviral expression of shRNA targeting BNIP3

   Depositor

   Joseph Gordon

   Insert

   Lenti-sh-BNIP3-FL
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6

   Available since

   Sept. 8, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRosetta

    Plasmid

    #59700

    Purpose

    Lentiviral vector for use as a standard for titering experiments when trying to compare vectors with different selection markers or to determine appropriate antibiotic conditions.

    Depositor

    John Doench, David Root

    Inserts

    Puro
    BlastR
    EGFP
    

    Use

    Lentiviral

    Tags

    2A

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLentiGuide-BlastR

    Plasmid

    #196970

    Purpose

    Lentiviral acceptor vector for pegRNAs with blasticidin resistance

    Depositor

    Leopold Parts

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    Aug. 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGGSG-FKBP-2HA-P2a-Pac

    Plasmid

    #235521

    Purpose

    C-term tagging construct: clone into PmlI and AfeI sites

    Depositor

    Brian Hendrich

    Insert

    FKBP
    

    Use

    Mouse Targeting

    Tags

    FKBP, 2xHA, Puro

    Expression

    Mutation

    None

    Promoter

    Available since

    May 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLEX_307

    Plasmid

    #41392

    Purpose

    Constitutive lentiviral expression, SV40-puro; EF1a-gateway-V5 tag

    Depositor

    David Root

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Lentiviral; Gateway destination vector

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Nov. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMKO.1 GFP

    Plasmid

    #10676

    Purpose

    Retroviral shRNA vector. Derivative of pMKO.1 puro (Addgene plasmid #8452) with GFP instead of puromycin resistance gene.

    Depositor

    William Hahn

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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15. CROP-seq-opti-dsRed

    Plasmid

    #201999

    Purpose

    Based on CROP-seq-opti (addgene #106280), we replaced the PURO resistance gene with a dsRed.

    Depositor

    Daniel Geschwind

    Type

    Empty backbone

    Use

    CRISPR and Lentiviral

    Tags

    dsRed

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    June 27, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGGSG-Bromo-T7-T2a-Pac

    Plasmid

    #235518

    Purpose

    Cloning vector for GGSG-BromoTag-T7-T2A-Pac. Clone into PmlI and AfeI

    Depositor

    Brian Hendrich

    Insert

    BromoTag
    

    Use

    Mouse Targeting

    Tags

    BromoTag, T7, Puro

    Expression

    Mutation

    None

    Promoter

    Available since

    May 29, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGGSG-Bromo-T7-P2a-Pac

    Plasmid

    #235517

    Purpose

    Cloning vector for GGSG-BromoTag-T7-P2A-Pac. Clone into PmlI and AfeI

    Depositor

    Brian Hendrich

    Insert

    BromoTag
    

    Use

    Mouse Targeting

    Tags

    BromoTag, T7, Puro

    Expression

    Mutation

    None

    Promoter

    Available since

    May 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLV-EF1a-V5-Tmem128

    Plasmid

    #120239

    Purpose

    Expresses V5-tagged Tmem128 in lentiviral vector

    Depositor

    Larry Gerace

    Insert

    Tmem128 (Tmem128 Mouse)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSpCas9(BB)-2A-Blast

    Plasmid

    #118055

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSpCas9(BB)-2A-Hygro

    Plasmid

    #127763

    Purpose

    Used for expression of sgRNA in mammalian cells

    Depositor

    Ken-Ichi Takemaru

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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