164,884 results

1. pX601-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA

   Plasmid

   #61591

   Purpose

   A single vector AAV-Cas9 system containing Cas9 from Staphylococcus aureus (SaCas9) and its sgRNA.

   Depositor

   Feng Zhang

   Article

   Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

   Inserts

   hSaCas9
   Chimeric guide for SaCas9
   

   Use

   AAV and CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG-loxPSTOPloxP-ZsGreen

   Plasmid

   #51269

   Purpose

   Cre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by loxP sites followed by ZsGreen

   Depositor

   Pawel Pelczar

   Article

   Hermann et al Nucleic Acids Res. 2014 Jan 9.

   Insert

   loxP-STOP-loxP-ZsGreen
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   March 17, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiGuide_DKO

   Plasmid

   #183193

   Purpose

   Plasmid with two U6 promoters and two gRNA scaffolds that allows for inserting two gRNAs for combinatorial CRISPR Screen or double-knock-out (DKO) screen

   Depositor

   Lang Li

   Article

   Tang et al STAR Protoc. 2022 Aug 24;3(3):101556. do

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hU6, mU6

   Available since

   Aug. 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-TRE-dTomato-GCaMP6s

   Plasmid

   #99864

   Purpose

   expresses dTomato and GCaMP6s under control of the Tet-off system

   Depositor

   Venkatesh Murthy

   Article

   Wallace et al Elife. 2020 Mar 9;9. pii: 50531. doi: 10

   Insert

   dTomato, GCaMP6s
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Tet-responsive promoter

   Available since

   Feb. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-hSyn-DIO-hM3D(Gq)-mCherry

   Plasmid

   #44361

   Purpose

   Double floxed Gq-coupled hM3D DREADD fused with mCherry under the control of human synapsin promoter.

   Depositor

   Bryan Roth

   Article

   Krashes et al J Clin Invest. 2011 Apr;121(4):1424-8. d

   Has Service

   AAV PHP.eB, AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9

   Insert

   hM3D(Gq)-mCherry
   

   Use

   AAV; Adeno associated viral vector

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   human Synapsin 1

   Available since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-hSyn-mCherry (AAV2)

   Viral Prep

   #114472-AAV2

   Purpose

   Ready-to-use AAV2 particles produced from pAAV-hSyn-mCherry (#114472). In addition to the viral particles, you will also receive purified pAAV-hSyn-mCherry plasmid DNA. Synapsin-driven mCherry control vector. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Karl Deisseroth

   Promoter

   Syn

   Tags

   mCherry

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

7. ppyCAG_RNaseH1_WT

   Plasmid

   #111906

   Purpose

   Express RNASEH1 in mammalian cell.

   Depositor

   Xiang-Dong Fu

   Article

   Chen et al Mol Cell. 2017 Nov 16;68(4):745-757.e5.

   Insert

   RNASEH1 (RNASEH1 Human)
   

   Use

   Tags

   V5

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV.TBG.PI.eGFP.WPRE.bGH

   Plasmid

   #105535

   Purpose

   AAV expression of EGFP from TBG promoter

   Depositor

   James M. Wilson

   Has Service

   AAV8

   Insert

   EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TBG

   Available since

   Feb. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAAV-hsyn-GRAB_DA2m (AAV9)

   Viral Prep

   #140553-AAV9

   Purpose

   Ready-to-use AAV9 particles produced from pAAV-hsyn-GRAB_DA2m (#140553). In addition to the viral particles, you will also receive purified pAAV-hsyn-GRAB_DA2m plasmid DNA. Synapsin-driven expression of 2nd generation medium affinity dopamine sensor GRAB_rDA2m. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Yulong Li

   Article

   Sun et al Nat Methods. 2020 Nov;17(11):1156-1166.

   Promoter

   Syn

   Tags

   None

   Available since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

10. pCDB327

    Plasmid

    #113671

    Purpose

    expresses Ulp1_R3 in Escherichia coli

    Depositor

    Christopher Bahl

    Article

    Lau et al J Biol Chem. 2018 Aug 24;293(34):13224-1

    Insert

    Ulp1_R3
    

    Use

    Tags

    6-His

    Expression

    Bacterial

    Mutation

    contains solubility enhancing mutations

    Promoter

    T7

    Available since

    Sept. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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