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pCfB5191

Plasmid

#126911

Purpose

Plasmid containing gRNA expression cassettes targeting the X-4 and XII-5 integration sites in Saccharomyces cerevisiae

Depositor

Irina Borodina

Publication

Milne et al FEMS Yeast Res. 2019 Sep 26. pii: foz062

Insert

X-4 and XII-5 gRNA

Use

CRISPR

Available since

Dec. 17, 2019

Availability

Academic Institutions and Nonprofits only

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EZ-L767

Plasmid

#131773

Purpose

PADH1_sFUS_Fusion Red_PixD_TACT1

Depositor

Jared Toettcher

Publication

Zhao et al Nat Chem Biol. 2019 Jun;15(6):589-597. d

Insert

PADH1_sFUS_Fusion Red_PixD_TACT1

Use

Integration into δ-sites

Expression

Yeast

Promoter

PADH1

Available since

Sept. 19, 2019

Availability

Academic Institutions and Nonprofits only

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pBSV2_PresT-mCherryBb

Plasmid

#118238

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes resT promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

resT promoter driven mCherry

Expression

Bacterial

Available since

Jan. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1819_pCR8-DR-SMN2-2-DR

Plasmid

#118653

Purpose

Transient transfection; Expressed DR-SMN2-DN2-DR CasRx gRNA with preprocessed DR 3'; Gateway Donor

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

DR-SMN2-2-DR

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pAC1818_pCR8-gRG6-SA

Plasmid

#118652

Purpose

Transient transfection; Expressed RG6-SA CasRx gRNA; Gateway Donor

Depositor

Albert Cheng

Publication

Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

gRG6-SA

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

Jan. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pXPR_sgGFP4

Plasmid

#119876

Purpose

gRNA expression vector

Depositor

William Hahn

Publication

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

sgGFP

Use

Lentiviral

Expression

Mammalian

Available since

Dec. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pGDP3 cat

Plasmid

#112888

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Publication

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

cat (Brevibacillus brevis) (cat Brevibacillus brevis)

Tags

6xHis

Expression

Bacterial

Promoter

Pbla

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pBSV2_P0526-mCherryBb

Plasmid

#118241

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0526 promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

bb0526 promoter driven mCherry

Expression

Bacterial

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pBSV2_P0031-mCherryBb

Plasmid

#118240

Purpose

Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes bb0031 promoter driven mCherry

Depositor

Christine Jacobs-Wagner

Publication

Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

Insert

bb0031 promoter driven mCherry

Expression

Bacterial

Available since

Nov. 21, 2018

Availability

Academic Institutions and Nonprofits only

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pDEW8

Plasmid

#84238

Purpose

Plasmid containing a P2A peptide (porcine teschovirus-1 derived) sequence that works efficiently in Saccharomyces cerevisiae

Depositor

David Weinberg

Publication

DiSanto et al Elife. 2016 Oct 1;5. pii: e20069. doi: 1

Insert

HA-mRuby-P2A-eGFP

Tags

HA-mRuby and eGFP

Expression

Yeast

Promoter

GPD

Available since

Nov. 7, 2016

Availability

Academic Institutions and Nonprofits only

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p3E-CMVmin:GFP no-pA

Plasmid

#80819

Purpose

CMVmin-promoted GFP without pA

Depositor

Philip Washbourne

Publication

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-CMV-GFP no pA

Expression

Mammalian

Promoter

CMV

Available since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pUB6_EPB49

Plasmid

#84015

Purpose

EPB49 minigene to test splicing event

Depositor

Robert Bradley

Publication

Ilagan et al Genome Res. 2015 Jan;25(1):14-26. doi: 1

Insert

EPB49 (DMTN Human)

Expression

Mammalian

Promoter

UB6

Available since

Oct. 17, 2016

Availability

Academic Institutions and Nonprofits only

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pETM6-H9-mCherry

Plasmid

#66532

Purpose

Expresses mCherry under the control of Mutant T7 Promoter H9

Depositor

Mattheos Koffas

Publication

Jones et al Sci Rep. 2015 Jun 11;5:11301. doi: 10.10

Insert

mCherry

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon Optimized for E. coli

Promoter

Mutant T7 Promoter - H9

Available since

July 16, 2015

Availability

Academic Institutions and Nonprofits only

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pGalT_EGFP-IRES_puro2b

Plasmid

#31804

Depositor

Daniel Gerlich

Publication

Guizetti et al Science. 2011 Mar 25;331(6024):1616-20.

Insert

pGALT

Expression

Mammalian

Mutation

*see comments

Available since

Oct. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pGL0_3 [RSF1010 ORI]

Plasmid

#198920

Purpose

Level 0 part

Depositor

Charlie Gilbert, Nili Ostrov, Cultivarium Tools

Publication

Gilbert et al bioRxiv 2023.05.19.541510

Insert

RSF1010 ORI

Use

Synthetic Biology

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pGDP3 aph(3'')-Ia

Plasmid

#112879

Purpose

This plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.

Depositor

Gerard Wright

Publication

Cox et al Cell Chem Biol. 2017 Jan 19;24(1):98-109

Insert

aph(3'')-Ia (SGR_RS01140 Streptomyces griseus)

Tags

6xHis

Expression

Bacterial

Promoter

Pbla

Available since

Dec. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pSe-Rp1

Plasmid

#111644

Purpose

For construction of spacer and repeat arrays for Sulfolobus islandicus REY154A

Depositor

Qunxin She

Publication

Peng et al Nucleic Acids Res. 2015 Jan;43(1):406-17

Type

Empty backbone

Use

Archaeal expression vector

Promoter

araS

Available since

July 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA5-FRT-TetO-fSNAPc

Plasmid

#106986

Purpose

open reading frame of fSNAP inserted into pcDNA5-FRT-TetO using the HindIII and XhoI restriction sites

Depositor

Melissa Moore

Publication

Braun et al Elife. 2018 Jun 22;7. pii: 37751. doi: 1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

fSNAP

Available since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pHM

Plasmid

#64173

Purpose

harbours marker genes HIS3 and MET17

Depositor

Markus Ralser

Publication

Mulleder et al F1000Res. 2016 Sep 20;5:2351. eCollectio

Type

Empty backbone

Expression

Bacterial and Yeast

Available since

Sept. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pCOLA-T7-WspR:G249A

Plasmid

#79163

Purpose

Expresses inactive WspR G249A under a T7 promoter

Depositor

Ming Hammond

Publication

Wang et al Nucleic Acids Res. 2016 Jul 5. pii: gkw5

Insert

WspR (wspR P. areuginosa)

Expression

Bacterial

Mutation

G249A

Promoter

T7

Available since

Aug. 12, 2016

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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