We narrowed to 10,675 results for: plasmids 101

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pAAV-AP.Agmat.1-GCaMP7f-WPRE

Plasmid

#211492

Purpose

expression of GCaMP7f under artificial promoter Agmat

Depositor

Georg Keller

Article

O'Toole et al Neuron. 2023 Sep 6:S0896-6273(23)00626-8

Insert

GCaMP7f

Use

AAV

Expression

Mammalian

Available Since

Dec. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pJKR-L-mphR

Plasmid

#62560

Purpose

Macrolide biosensor. Low copy plasmid that expresses GFP proportional to macrolide concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pGL4_NFAT-RE-CMV-BC0527-luc2

Plasmid

#214657

Purpose

barcoded assay, Ca2+ sensor

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

6x clustered NFAT element with barcode 0527

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCJH059_proD_Cas12c_DualRNA-GFP_CAM

Plasmid

#183076

Purpose

Plasmid expressing Cas12c tracrRNA and a GFP-targeting pre-crRNA separately.

Depositor

Jennifer Doudna

Article

Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

Insert

Cas12c tracrRNA and pre-crRNA

Use

CRISPR

Expression

Bacterial

Available Since

June 2, 2022

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Pink

Plasmid

#160445

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Pink chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Pink

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Jan. 20, 2021

Availability

Academic Institutions and Nonprofits only

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YIplac211-GGA2-msGFPx3

Plasmid

#105258

Purpose

Plasmid for pop-in/pop-out replacement of Gga2 with Gga2-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Gga2 portion (GGA2 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pSB1C3-J23110-B0034-mRFP1_Orange

Plasmid

#160444

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses mRFP1_Orange chromoprotein in E. coli

Depositor

Anthony Forster

Article

Bao et al Anal Biochem. 2020 Sep 3:113936. doi: 10

Insert

promoter, RBS, mRFP1E_Orange

Use

Synthetic Biology

Mutation

BioBrick sites removed

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pGL4_CRE-CMV-BC0482-luc2

Plasmid

#214635

Purpose

barcoded assay, cAMP/Ca2+ sensor

Depositor

Michael Wehr

Article

Popovic et al STAR Protoc. 2024 Apr 16;5(2):102987. do

Insert

6x clustered CRE element with barcode 0482

Use

Luciferase

Expression

Mammalian

Available Since

Feb. 26, 2024

Availability

Academic Institutions and Nonprofits only

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pDB026a

Plasmid

#109260

Purpose

Accessory plasmid for PACE of MjTyrRS variants

Depositor

David Liu

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Ppsp_pIII(P29*); luxAB; PproK_TyrT(Opt, CUA);

Expression

Bacterial

Mutation

amber codon at position 29 gIII

Promoter

Ppsp; PproK

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL2-iGFPx6

Plasmid

#105251

Purpose

Plasmid for pop-in/pop-out replacement of Apl2 with Apl2-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

APL2 portion (APL2 Budding Yeast)

Tags

iGFPx6

Expression

Yeast

Available Since

June 8, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL5-mCherry2Bx6

Plasmid

#105253

Purpose

Plasmid for pop-in/pop-out replacement of Apl5 with Apl5-mCherry2Bx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Apl5 (APL5 Budding Yeast)

Tags

mCherry2Bx6

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pJKR-L-ttgR

Plasmid

#62566

Purpose

Flavonoid biosensor. Low copy plasmid that expresses GFP proportional to flavonoid concentration.

Depositor

George Church

Article

Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

Insert

GFP

Expression

Bacterial

Available Since

Aug. 13, 2015

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL6-msGFPx3

Plasmid

#105254

Purpose

Plasmid for pop-in/pop-out replacement of Apl6 with Apl6-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Apl6 portion (APL6 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-CHC1-msGFPx3

Plasmid

#105256

Purpose

Plasmid for pop-in/pop-out replacement of Chc1 with Chc1-msGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Chc1 portion (CHC1 Budding Yeast)

Tags

msGFPx3

Expression

Yeast

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pRF TpC RE8

Plasmid

#171047

Purpose

Firefly-Renilla luciferase assay for hTERT promoter activity of RE8

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

RE8

Use

Luciferase

Expression

Mammalian

Available Since

Aug. 4, 2021

Availability

Academic Institutions and Nonprofits only

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pLV VEET-GFP

Plasmid

#188706

Purpose

Lentivirus transfer plasmid encoding VEET-GFP

Depositor

David Tobin

Article

Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

Insert

VEET-GFP

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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YIplac211-APL5-iGFPx6

Plasmid

#105252

Purpose

Plasmid for pop-in/pop-out replacement of Apl5 with Apl5-iGFPx6.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Apl5 (APL5 Budding Yeast)

Tags

iGFPx6

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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YIplac211-SNX4-iGFPx3

Plasmid

#105269

Purpose

Plasmid for pop-in/pop-out replacement of Snx4 with Snx4-iGFPx3.

Depositor

Benjamin Glick

Article

Day et al Dev Cell. 2018 Jan 8;44(1):56-72.e4. doi

Insert

Snx4 portion (SNX4 Budding Yeast)

Tags

iGFPx3

Expression

Yeast

Available Since

Jan. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pAP685-2

Plasmid

#99490

Purpose

TEV::eGFP::ER::6xHis::linker::3XFlag

Depositor

Geraldine Seydoux

Article

Paix et al Methods. 2017 May 15;121-122:86-93. doi:

Insert

TEV::eGFP::RE::6Xhis::linker::3XFlag

Expression

Bacterial

Available Since

March 1, 2018

Availability

Academic Institutions and Nonprofits only

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pRF TpC RE2

Plasmid

#171041

Purpose

Firefly-Renilla luciferase assay for hTERT promoter activity of RE2

Depositor

Agnel Sfeir

Article

Penev et al Mol Cell. 2021 Apr 6. pii: S1097-2765(21

Insert

RE2

Use

Luciferase

Expression

Mammalian

Available Since

May 4, 2022

Availability

Academic Institutions and Nonprofits only

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