-
Plasmid#71409PurposeThere is no gene/insert. It is a backbone plasmid that others can use to insert sgRNAs of interest. The cloning site for this BsmBIDepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJan. 14, 2016AvailabilityAcademic Institutions and Nonprofits only
-
LC3B sgRNA
Plasmid#207556PurposepX330 expressing Cas9 and a sgRNA targeting the LC3B locusDepositorInsertCACTGAATACCAGCGCCTAG
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
L4440_BioBrick-sgRNA
Plasmid#177783PurposeOverexpression of sgRNAs in E. coli HT115, sgRNA overexpression scaffold; for ingestion by C. elegansDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLX-sgRNA
Plasmid#50662PurposeLentiviral expression of AAVS1-targeting sgRNA; insert can be replaced with custom sgRNA (see protocol)DepositorInsertsgAAVS1
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDT-sgRNA
Plasmid#138271PurposeDual-targeting sgRNA vector that contains both a sgRNA against BFP (sgBG) and a sgRNA for a genomic target site (sgTS)DepositorInsertsgBG, sgTS
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceMarch 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcU6_1 sgRNA
Plasmid#92395PurposeChicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_1 pol III promoter.DepositorInsertchick U6.1 promoter and gRNA cloning cassette
UseCRISPRTagsExpressionMammalianMutationPromoterchick U6.1Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcU6_3 sgRNA
Plasmid#92359PurposeChicken-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter.DepositorInsertchick U6.3 promoter and gRNA cloning cassette
UseCRISPRTagsExpressionMammalianMutationPromoterchick U6.3Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
ATG9A sgRNA
Plasmid#207557PurposepX330 expressing Cas9 and a sgRNA targeting the ATG9A locusDepositorInsertCACTGAATACCAGCGCCTAG
UseTagsExpressionMammalianMutationPromoterCMV and U6Available sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCF142_U6-sgRNA
Plasmid#225960PurposeU6-sgRNA (recipient). Expresses U6-sgRNA (recipient) for VLP production. This is a recipient vector for cloning of specific SpyCas9 sgRNAs.DepositorInsertU6-sgRNA (recipient)
UseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pTAKN2_J23119-sgRNA
Plasmid#108552PurposeBacterial sgRNA expression vectorDepositorInsert2x Streptococcus pyogenes sgRNA
UseTagsExpressionBacterialMutationPromoterJ23119Available sinceMay 3, 2018AvailabilityAcademic Institutions and Nonprofits only