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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 1,283 results for: abo.1

Showing: 31 - 60 of 1283 results

  1. Cer(1-158)-gamma-1 in pcDNAI/Amp

    Plasmid
    #55623
    Purpose
    An amino-terminal mCerulean fragment was fused to Ggamma-1. When co-expressed with a carboxyl-terminal CFP fragment fused to a Gbeta subunit with which it interacts, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Mervine et al Mol Pharmacol. 2006 Jul;70(1):194-205. E
    Insert
    Cer(1-158)-gamma-1 (GNGT1 Bovine, Aequorea victoria)
    Use
    Tags
    Cer(1-158) was fused to the amino terminus of Gga…
    Expression
    Mammalian
    Mutation
    Ggamma-1 was amplified by PCR, which added a BamH…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55623-plasmid-map-sequence-id-115166

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  2. YFP(1-158)-beta-1 in pcDNAI/Amp

    Plasmid
    #54468
    Purpose
    An amino-terminal YFP fragment was fused to Gbeta-1. When co-expressed with a carboxyl-terminal YFP or CFP fragment fused to a Ggamma subunit, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Hynes et al J Biol Chem. 2004 Jul 16;279(29):30279-8
    Insert
    YFP (1-158)/beta-1 (GNB1 Aequorea victoria, Human)
    Use
    Tags
    YFP(1-158) was fused to the amino terminus of Gbe…
    Expression
    Mammalian
    Mutation
    GBeta-1 was amplified via PCR, which removed an i…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    54468-plasmid-map-sequence-id-112586

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  3. pMW-PahZ1 KT-1

    Plasmid
    #141134
    Purpose
    Expresses in BL-21 (DE3) cells to produce the poly(aspartic acid) hydrolase-1 from Sphingomonas Sp. KT-1 which selectively cleaves the beta linkages in thermally synthesized poly(aspartic acid).
    Depositor
    Mitch Weiland
    Article
    Bolay et al Journal of Chemical Education
    Insert
    PahZ1
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Not the full-length gene. PahZ1 fragment starts a…
    Promoter
    Available since
    Sept. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    141134-plasmid-map-sequence-id-308652

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  8. Cer(1-158)-RGS7

    Plasmid
    #55760
    Purpose
    An amino-terminal mCerulean fragment was fused to RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    mCer(1-158)-RGS7 (RGS7 Aequorea victoria, Human)
    Use
    Tags
    Cer(1-158) was fused to the amino terminus of RGS…
    Expression
    Mammalian
    Mutation
    RGS7 was amplified via PCR, which added an N-term…
    Promoter
    CMV
    Available since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    55760-plasmid-map-sequence-id-115314

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  11. CFP(1-158)-RGS7t

    Plasmid
    #55761
    Purpose
    An amino-terminal CFP Fragment was fused to residues 202-477 of RGS7. When co-expressed with a carboxyl terminal fragment of CFP fused to Gbeta-5, a fluorescent signal is produced.
    Depositor
    Catherine Berlot
    Article
    Yost et al Mol Pharmacol. 2007 Oct;72(4):812-25. Ep
    Insert
    RGS7(202-477)-CFP(1-158) (RGS7 Aequorea victoria, Human)
    Use
    Tags
    CFP(1-158) was fused to the amino terminus of RGS…
    Expression
    Mammalian
    Mutation
    The RGS sequence amino terminal to the GGL domain…
    Promoter
    CMV
    Available since
    Aug. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    55761-plasmid-map-sequence-id-89566

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  12. lis-1::L4440

    Plasmid
    #11336
    Depositor
    Guy Caldwell
    Insert
    lis-1 (lis-1 Nematode)
    Use
    RNAi
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only

  13. nud-1::L4440

    Plasmid
    #11337
    Depositor
    Guy Caldwell
    Insert
    nud-1 (nud-1 Nematode)
    Use
    RNAi
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 12, 2006
    Availability
    Academic Institutions and Nonprofits only

  14. pLexA::nud-1

    Plasmid
    #11339
    Depositor
    Guy Caldwell
    Insert
    nud-1 (nud-1 Nematode)
    Use
    Tags
    LexA DNA Binding Domain
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only

  15. pLexA::lis-1

    Plasmid
    #11338
    Depositor
    Guy Caldwell
    Insert
    lis-1 (lis-1 Nematode)
    Use
    Tags
    LexA DNA Binding Domain
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 25, 2006
    Availability
    Academic Institutions and Nonprofits only

  16. J-SPRTNC-1

    Plasmid
    #166521
    Purpose
    scFv of a human scaffold targeting SprT-like N-terminal domain. Antigen coverage aa 1-216 of 489
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 31, 2021
    Availability
    Academic Institutions and Nonprofits only
    166521-plasmid-map-sequence-id-329135

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  17. J-DHTKD1A-1

    Plasmid
    #166524
    Purpose
    scFv of a human scaffold targeting Dehydrogenase E1 and transketolase domain containing protein 1. Antigen coverage aa 45-919 of 919
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 20, 2021
    Availability
    Academic Institutions and Nonprofits only
    166524-plasmid-map-sequence-id-329137

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  18. Z-QARS-1

    Plasmid
    #166557
    Purpose
    scFv of a human scaffold targeting Glutamine-tRNA synthetase. Antigen coverage aa 1-775 of 775, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166557-plasmid-map-sequence-id-329265

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  19. O-FHL1-1

    Plasmid
    #166543
    Purpose
    scFv of a human scaffold targeting Four and a half LIM domains protein 1. Antigen coverage aa 1-280 aa, full-length
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    March 24, 2021
    Availability
    Academic Institutions and Nonprofits only
    166543-plasmid-map-sequence-id-328279

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  20. Z-AIMP1-1

    Plasmid
    #166560
    Purpose
    scFv of a human scaffold targeting Aminoacyl tRNA synthase complex-interacting multifunctional protein 1. Antigen coverage aa 147-312 of 312
    Depositor
    Susanne Gräslund
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 2, 2021
    Availability
    Academic Institutions and Nonprofits only
    166560-plasmid-map-sequence-id-329268

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  21. O-DARS-1

    Plasmid
    #166538
    Purpose
    scFv of a human scaffold targeting Aspartyl-tRNA synthetase. Antigen coverage aa 1-501 of 501, full-length
    Depositor
    Susanne Gräslund
    Article
    Preger et al J Biol Chem. 2020 Oct 9;295(41):13981-13
    Insert
    single chain-Fv, scFv
    Use
    Recombinant antibody expression
    Tags
    3xFLAG, His6 and OmpA leader sequence
    Expression
    Bacterial
    Mutation
    Promoter
    LacZ
    Available since
    April 1, 2021
    Availability
    Academic Institutions and Nonprofits only
    166538-plasmid-map-sequence-id-329231

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Showing: 31 - 60 of 1283 results