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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 2,492 results for: MEA

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Showing: 41 - 80 of 2492 results

  3. pCL21-decoder

    Plasmid
    #159448
    Purpose
    Insert: Pact1-lexA-ER-B112-phosphodegron-tCyc1. The transcription factor LexA-ER-B112 (see addgene FRP880) is degraded upon addition of alpha factor. Backbone: pRG201 (addGene). Marker: Met15.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    Pact1-lexA-ER-B112-phosphodegron-tCyc1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    act1
    Available Since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159448-plasmid-map-sequence-id-313196

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  4. pCL33-decoder

    Plasmid
    #159451
    Purpose
    Insert: Pfus1mut-tetR-nls-malE-tCyc1. TetR-NLS-MBP (Azizoglu et al, bioRxiv 2019) is inducible by alpha-factor. Backbone: pRG203MX (addgene). Marker: His3.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    Pfus1mut-tetR-nls-malE-tCyc1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    Pfus1mut
    Available Since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159451-plasmid-map-sequence-id-313201

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  5. pCL38-decoder

    Plasmid
    #159452
    Purpose
    Insert: Pfus1tet-lexA-ER-B112-tCyc1.The transcription factor LexA-ER-B112 (see addgene FRP880) is inducible by alpha factor, and repressible by TetR. Backbone: pRG235 (addgene). Marker: Leu2
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    Pfus1tet-LexA-ER-B112-tCyc1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    Pfus1tet
    Available Since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159452-plasmid-map-sequence-id-313203

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  6. pCL12-decoder

    Plasmid
    #159447
    Purpose
    Insert: PlexAtet-citrine-tCyc1. The fluorescent protein Citrine is inducible by LexA-ER-B112 upon addition of estradiol, and repressible by TetR. Backbone: pRG207 (addGene). Marker:Lys2.
    Depositor
    Joerg Stelling
    Article
    Lormeau et al Nat Commun. 2021 Mar 25;12(1):1886. doi:
    Insert
    PlexAtet-Citrine-tCyc1
    Use
    Synthetic Biology
    Expression
    Yeast
    Promoter
    PlexAtet
    Available Since
    Dec. 11, 2020
    Availability
    Academic Institutions and Nonprofits only
    159447-plasmid-map-sequence-id-313193

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  9. p426MET25_SSKar2-sfpHluorin-HDEL (ER-sfpHluorin, MRV66)

    Plasmid
    #115698
    Purpose
    ER-targeted superfolder pHluorin for improved pH measurements in the ER of S. cerevisiae
    Depositor
    Eckhard Boles
    Article
    Reifenrath et al Sci Rep. 2018 Aug 10;8(1):11985. doi: 10
    Insert
    SSKar2-sfpHluorin-HDEL
    Tags
    ER retention signal containing HDEL and ER signal…
    Expression
    Yeast
    Mutation
    for introduced mutations see publication
    Promoter
    MET25
    Available Since
    Sept. 27, 2018
    Availability
    Academic Institutions and Nonprofits only
    115698-plasmid-map-sequence-id-223642

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  28. Methyl-binding domain protein 2 (MBD2) coiled-coil domain

    Plasmid
    #132721
    Purpose
    Bacterial expression vector for methyl-binding domain protein 2 (MBD2) coiled-coil domain for use as competitor peptide in measurements of effective concentrations
    Depositor
    Magnus Kjaergaard
    Article
    Sorensen et al Methods Mol Biol. 2020;2141:505-518. doi
    Insert
    Methyl-binding protein domain 2 coiled-coil domain
    Tags
    N-terminal His-tag. Flanking linkers to match acc…
    Expression
    Bacterial
    Promoter
    T/
    Available Since
    Oct. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    132721-plasmid-map-sequence-id-260259

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  39. N-BLa 1.2

    Plasmid
    #88999
    Purpose
    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the N-terminal fragment of the split β-lactamase.
    Depositor
    Dieter Langosch
    Article
    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103
    Insert
    N-BLa 1.2 fusion protein
    Tags
    Flag
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    May 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    88999-plasmid-map-sequence-id-179971

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  40. C-BLa 1.2

    Plasmid
    #89000
    Purpose
    BLaTM, a genetic tool to measure homo- and heterotypic transmembrane helix-helix interactions. Plasmid encoding the 1.2 fusionprotein containing the C-terminal fragment of the split β-lactamase.
    Depositor
    Dieter Langosch
    Article
    Schanzenbach et al Sci Rep. 2017 Mar 7;7:43476. doi: 10.103
    Insert
    C-BLa 1.2 fusion protein
    Tags
    Flag
    Expression
    Bacterial
    Promoter
    araBAD
    Available Since
    May 2, 2017
    Availability
    Academic Institutions and Nonprofits only
    89000-plasmid-map-sequence-id-177513

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Showing: 41 - 80 of 2492 results