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Plasmid#199207PurposeUsed for the expression of TBK1 carrying E696K mutation (SMC Internal No. 1998)DepositorInsertTBK1 (TBK1 Human)
UseRetroviralTagsExpressionMammalianMutationE696KPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBMN_HA-TBK1 (kinase dead - D135N)
Plasmid#199206PurposeUsed for the expression of TBK1 carrying a kinase dead mutation D135N (SMC Internal No. 1997)DepositorInsertTBK1 (TBK1 Human)
UseRetroviralTagsExpressionMammalianMutationD135NPromoterAvailable sinceMay 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1
Plasmid#187830PurposePlasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631DepositorInsertTBK1 (TBK1 Human)
UseTagsGST and TEV-mEGFP-GG LinkerExpressionBacterial and InsectMutationPromoterAvailable sinceOct. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1ΔCTD (res 1-666)
Plasmid#198072PurposeUsed for the expression and purification of EGFP labelled TBK1 carrying a mutation for the removal of the CTD (SMC Internal No. 1874)DepositorInsertTBK1 (TBK1 Human)
UseTagsGST-TEV-EGFPExpressionInsectMutationTBK1ΔCTD (res 1-666)PromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac_Dual_GST-TEV-EGFP-TBK1-E696K
Plasmid#198037PurposeUsed for the expression and purification of EGFP labelled TBK1 carrying E696K mutation (SMC Internal No.: 1649)DepositorInsertTBK1 (TBK1 Human)
UseTagsGST-TEV-EGFPExpressionInsectMutationE696KPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-GST-TEV-mCherry-TBK1
Plasmid#198033PurposeUsed for the expression and purification of mCherry labelled TBK1 (SMC Internal No.: 1675)DepositorInsertTBK1 (TBK1 Human)
UseTagsGST-TEV and mCherryExpressionInsectMutationE696KPromoterAvailable sinceApril 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
R777-E341 Hs.TBK1
Plasmid#70625PurposeGateway ORF clone of human TBK1 [NM_013254.3] with stop codon (for native or N-terminal fusions)DepositorInsertTBK1 (TBK1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
R777-E342 Hs.TBK1-nostop
Plasmid#70626PurposeGateway ORF clone of human TBK1 [NM_013254.3] without stop codon (for C-terminal fusions)DepositorInsertTBK1 (TBK1 Human)
UseGateway entry cloneTagsExpressionMutationPromoterAvailable sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
TBK1_Halo_C_allele
Plasmid#178172PurposeDonor vector for endogenous tagging of human TBK1 at the C-terminus with halotagInsertHalotag (TBK1 Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pC004 - PFS library in Beta-lactamase gene Target Plasmid
Pooled Library#79153PurposePFS library inserted at the 5' of the Beta-lactamase gene for screening PFS preferenceDepositorAvailable sinceOct. 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNR4A1.1.0-gDNA
Plasmid#112391PurposeCRISPR plasmid for expression of Cas9 and gRNA targeting human transcription factor NR4A1DepositorInsertNR4A1 (NR4A1 Human)
UseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceDec. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pNR4A1-donor
Plasmid#112295PurposeCRISPR donor plasmid to tag human transcription factor NR4A1 with GFPDepositorInsertNR4A1 homology arms flanking EGFP-IRES-Neo cassette (NR4A1 Human)
UseCRISPRTagsExpressionMutationhg38:12:52058840:C>T, hg38:12:52058930:A>C,…PromoterAvailable sinceNov. 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSUPER TBK1
Plasmid#26210DepositorInsertpSUPER TBK1 (TBK1 Human)
UseRNAiTagsExpressionMutationPromoterAvailable sinceAug. 24, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-hTBK1-EGFP
Plasmid#221553PurposeExpresses GFP-tagged human TBK1 in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertTBK1 (TBK1 Human)
UsePiggybacTagsGFPExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLKO.1 puro_shTBK1_3185
Plasmid#167294PurposeLentiviral plKO.1 construct coding for a short-hairpin RNA targeting the human gene TBK1. Contains a puro resistance cassette.DepositorInsertshRNA TBK1 (TBK1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac-Dual- GST-TEV-TBK1
Plasmid#208875PurposeFor expression in insect cells of TBK1DepositorInsertTBK1 (TBK1 Human)
UseTagsGST-TEVExpressionInsectMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSpCas9-2A-Puro-TBK1-gRNA (PX459)
Plasmid#221550PurposeExpresses Cas9 and gRNA for disruption of TBK1 gene in human cellsDepositorInsertgRNA targeting human TBK1 (TBK1 Human)
UseTagsExpressionMammalianMutationPromoteru6Available sinceJuly 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-TSF-TBK1
Plasmid#216709PurposeMammalian expression of TBK1DepositorInsertTBK1 (TBK1 Human)
UseTagsTSFExpressionMammalianMutationPromoterAvailable sinceNov. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pPB-EF1A-Puro-hTBK1-E696K-EGFP
Plasmid#221554PurposeExpresses GFP-tagged human TBK1 with E696K mutation in mammalian cells. Compatible with piggybac transposase for genome integration.DepositorInsertTBK1 (TBK1 Human)
UsePiggybacTagsGFPExpressionMammalianMutationChanged glutamate 696 to lysinePromoterEF1aAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only