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ADH1pro_GCN4_SIR4
Plasmid#138620PurposeTranscription factor tagged with Sir4 used for Calling Cards experiments for binding measurementDepositorInsertGCN4 (GCN4 Budding Yeast)
UseTagsExpressionYeastMutationPromoterADH1Available sinceJune 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
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TmSir2
Plasmid#25815DepositorInsertTm Sir2
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
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sgFFL_3x_siRNA
Plasmid#59893Purposesynthetic autoregulatory gene circuit made by inserting an intron containing the mouse mir-124–3 gene into mCherry. Contains the miR-124-regulated 3′UTR of the Vamp3 gene with three siRNA-like sitesDepositorInsertmCherry with intron containing the mouse mir-124–3
UseTagsPEST and VAMP 3 UTRExpressionMammalianMutationVAMP 3 UTR--has three siRNA-like targets (fully c…Promoterdoxycycline (Dox)-inducible promoter (CMV/TO)Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
ADH1pro_GCR2_SIR4
Plasmid#138619PurposeTranscription factor tagged with Sir4 used for Calling Cards experiments for binding measurementDepositorInsertGCR2 (GCR2 Budding Yeast)
UseTagsExpressionYeastMutationPromoterADH1Available sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
ADH1pro_GCR1_SIR4
Plasmid#138618PurposeTranscription factor tagged with Sir4 used for Calling Cards experiments for binding measurementDepositorInsertGCR1 (GCR1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterADH1Available sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
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ADH1pro_CBF1_SIR4
Plasmid#138616PurposeTranscription factor tagged with Sir4 used for Calling Cards experiments for binding measurementDepositorInsertCBF1 (CBF1 Budding Yeast)
UseTagsExpressionYeastMutationPromoterADH1Available sinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEAHISSIRV131
Plasmid#97053PurposeExpressing Sulfolobus islandicus rudivirus (SIRV) orf 131 proteinDepositorInsertSIRV orf 131
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionBacterialMutationnonePromoterT7Available sinceDec. 13, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEHISSIRV119
Plasmid#97054PurposeExpressing Sulfolobus islandicus rudivirus (SIRV) orf 119 proteinDepositorInsertSIRV orf 119
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEHISSIRV55
Plasmid#97055PurposeExpressing Sulfolobus islandicus rudivirus (SIRV) orf 55 proteinDepositorInsertSIRV orf 55
UseTagsN-terminal TEV protease cleavable 6xHis tagExpressionMutationnonePromoterT7Available sinceAug. 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
TbSIR2RP1
Plasmid#67242PurposeDetermine its activity and specificityDepositorInsertTbSIR2RP1
UseTagsMBP, TEV, Thrombin, HIS, and noneExpressionBacterialMutationPromoterp-lacAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
PP-SIR2
Plasmid#25204PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertPP-SIR2:K10-M273 (Sir2 Plasmodium falciparum)
UseTagsHisExpressionBacterialMutationContains amino acids K10-M273PromoterAvailable sinceAug. 12, 2010AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSIRV-NF-kB-eCFP
Plasmid#118094PurposeFlourescent reporter for NF-kB activityDepositorInserteCFP
UseRetroviralTagsExpressionMutationPromoterminimal promoter with NF-kB responsive elementAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSIRV-AP-1-mCherry
Plasmid#118095PurposeFluorescent reporter for AP-1 activationDepositorInsertmCherry
UseRetroviralTagsExpressionMutationPromoterminimal promoter with AP-1 responsive elementAvailable sinceNov. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSIRV-NF-kB-eGFP
Plasmid#118093PurposeFluorescent reporter for NF-kB activityDepositorInserteGFP
UseRetroviralTagsExpressionMutationPromoterminimal promoter with NF-kB responsive elementAvailable sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only
