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Plasmid#229996PurposeExpresses anti-GFP PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-LaG2-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-VEGF(Nb35) PAGER(TF)
Plasmid#230000PurposeExpresses anti-VEGF PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-Nb35-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-TNFalpha(Ozoralizumab) PAGER(TF)
Plasmid#230001PurposeExpresses anti-TNFalpha PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-Ozoralizumab-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-CCL2(8E10) PAGER(TF)
Plasmid#230002PurposeExpresses anti-CCL2 PAGER(TF) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-8E10-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-mCherry(LaM8) PAGER(TF)
Plasmid#229999PurposeExpresses anti-mCherry PAGER(TF) (high reversibility) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-LaM8-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV-anti-mCherry(LaM6) PAGER(TF)
Plasmid#229998PurposeExpresses anti-mCherry PAGER(TF) (high sensitivity) in mammalian cells; used with NanoLuc-Arrestin-TEVp (Addgene #125228) and UAS-Firefly Luciferase reporter (Addgene #104840)DepositorInsertIL2SP-Aro6-LaM6-TEVcs-ALFA-KORD(RAA)-LOV-TEVcs-Gal4
UseAAVTagsExpressionMammalianMutationV360A/R361A on KORDPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNTI751 pPGK1-GCN4(UTR)-ZEM(TF)
Plasmid#164916PurposeExpresses the ZEM transcription factor under the translational control of the yeast GCN4 5'UTRDepositorInsertGCN4(UTR)-ZEM(TF)
UseSynthetic BiologyTagsExpressionYeastMutationPromoterPGK1 (yeast)Available sinceFeb. 18, 2021AvailabilityAcademic Institutions and Nonprofits only -
BPV00500 pET21GG2 His(6)-TF-BECN(1-450)
Plasmid#136684PurposeExpresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor)DepositorInsertBeclin-1 (BECN1 Human)
UseTagsTrigger FactorExpressionBacterialMutationDNA optimized for E. coli expressionPromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
p20 Inducible TFs for G-integration: Lex-ER-B112 and ZPM
Plasmid#183150PurposeExpression cassettes for transcription factor induced by estradiol (Lex-ER-B112) or progesterone (ZPM)DepositorInsertsLex-ER-B112
Zif268 DBD - hPRLBD - MSN2AD
UseFor yeast genomic integrationTagsExpressionMutationPromoterAvailable sinceJuly 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
BPV00914 pET21GG2 H6-TF-BECN (1-450) [VA-MA-LA]
Plasmid#136688PurposeExpresses the full-length Beclin-1 protein (a.a. 1-450) with a N-terminal hexa-Histidine and solubility tag (i.e., Trigger Factor). Three mutations are added to the CC and ECD/BARA interface.DepositorInsertBeclin-1 (BECN1 Human)
UseTagsTrigger FactorExpressionBacterialMutationDNA optimized for E. coli expression w/ three mut…PromoterT7Available sinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-SS-248
Plasmid#68762PurposeZF9-TF-V3 with mCherry markerDepositorInsertsZF9-TF-V3
ZF9-TF-V3
mCherry
UseTagsNLS and flagExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCFT4.2
Plasmid#208807PurposeExpresses the fluorescent protein phiLOV2.1DepositorInsertphiLOV2.1
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
CLYBL hOPM
Plasmid#112499PurposeHomologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in CLYBL locusDepositorUseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAVS hOPM
Plasmid#112498PurposeHomologous recombination vector for dox-inducible overexpression of OTX2, PAX6, and MITF in AAVS locus.DepositorUseTagsExpressionMammalianMutationPromoterTRE3GAvailable sinceMarch 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCFT9.2
Plasmid#208809PurposeExpresses the fluorescent protein GFPmut2DepositorInsertGFPmut2
UseTagsExpressionMutationPromotertacAvailable sinceOct. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCFT3.2
Plasmid#208806PurposeExpresses the fluorescent protein iLOVDepositorInsertiLOV
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(-) HuTF-Fc-His tag
Plasmid#183253PurposeSoluble expression of the extracellular domains of human tissue factor (TF)-FcDepositorInsertTissue factor (F3 Human)
UseTags8xHisExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCFT7.2
Plasmid#208808PurposeExpresses the fluorescent protein FASTDepositorInsertFAST
UseTagsExpressionBacterialMutationPromotertacAvailable sinceOct. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBW121-SS-273
Plasmid#68763PurposeZF9-TF-V2 with N-FLAG and C-HISDepositorInsertZF9-TF-V2
UseTagsHIS, NLS, and flagExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pVITRO1-SS-247
Plasmid#68761PurposeZF9-TF-V2 with mCherry markerDepositorInsertsZF9-TF-V2
mCherry
UseTagsNLS and flagExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2015AvailabilityAcademic Institutions and Nonprofits only