We narrowed to 962 results for: cas12a

1. pCas12a

   Plasmid

   #186446

   Purpose

   Encodes Cas12a from F. novicida (type V-A)

   Depositor

   John van der Oost

   Article

   Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

   Insert

   Cas12a
   

   Use

   CRISPR

   Promoter

   T7

   Available Since

   March 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pNOC_hfnCas12a_NR1_NR2_LPT1

   Plasmid

   #176253

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene and one spacer targeting the LPAAT gene

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Available Since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCJH027_SC101_pLAC_LbCas12a_Kan

   Plasmid

   #183074

   Purpose

   Wild-type LbCas12a (Chen et al., 2018) in a plasmid containing a SC101 origin and lac promoter for in vivo interference assay.

   Depositor

   Jennifer Doudna

   Article

   Huang et al Mol Cell. 2022 Jun 2;82(11):2148-2160.e4

   Insert

   LbCas12a
   

   Expression

   Bacterial

   Mutation

   Wild-type

   Available Since

   June 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNOC_hfnCas12a_NR1_NR2

   Plasmid

   #176252

   Purpose

   pNOC episomal plasmid harboring the humanized fnCas12a gene sequence tagged with Nlux and CRISPR array with spacers 1 and 2 targeting the Nitrate reductase gene of N. oceanica IMET1 separated by full

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Available Since

   Nov. 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. ttHsCas12a

   Plasmid

   #182385

   Purpose

   Gives high editing efficiency in barley.

   Depositor

   Wendy Harwood

   Article

   Lawrenson et al bioRxiv 2022.04.28.489853

   Insert

   ttHsCas12a
   

   Use

   CRISPR; Coding sequence only

   Mutation

   D156R

   Promoter

   None

   Available Since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. LbCas12a

   Plasmid

   #188493

   Purpose

   Expresses FLAG tagged LbCas12a

   Depositor

   Isaac Hilton

   Article

   Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

   Insert

   Cas12a
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNOC_dfnCas12a_crRNA NC

   Plasmid

   #176256

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and spacer sequence is replaced by the type IIS restriction site for endonuclease BaeI that can be

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Mutation

   D917A and E1006A mutations to inactivate the endo…

   Available Since

   Oct. 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQdCas12a.empty

   Plasmid

   #236036

   Purpose

   The plasmid pQdCas12a.empty expresses the dCas12a endonuclease.

   Depositor

   Radhakrishnan Mahadevan

   Article

   Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. pQdCas12a.sggfp(A)

   Plasmid

   #236038

   Purpose

   The plasmid pQdCas12a.sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene.

   Depositor

   Radhakrishnan Mahadevan

   Article

   Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

   Insert

   quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (A) of gfp gene
   

   Use

   CRISPR

   Promoter

   quorum sensing promoter

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pQdCas12a.sggfp(B)

    Plasmid

    #236040

    Purpose

    The plasmid pQdCas12a.sggfp(B) expresses the dCas12a endonuclease and the sgRNA (design B) targeting the gfp gene.

    Depositor

    Radhakrishnan Mahadevan

    Article

    Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

    Insert

    quorum sensing cassette luxI, mutated luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (B) of gfp gene
    

    Use

    CRISPR

    Promoter

    quorum sensing promoter

    Available Since

    April 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. MbCas12a_K487_GPG_GR2

    Plasmid

    #231577

    Purpose

    Encodes a cortisol inducible variant of MbCas12a

    Depositor

    Dominik Niopek

    Article

    Wolf et al bioRxiv 2024.12.04.626757

    Insert

    MbCas12a with a GR2 insertion behind K487
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Feb. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. LbCas12a-niCPE

    Plasmid

    #213747

    Purpose

    For circular RNA-mediated prime editor using LbCas12a-niCPE in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    MCP-nLbCas12a-M-MLV RT∆RNase H
    

    Use

    CRISPR

    Tags

    BPNLS

    Expression

    Mammalian

    Mutation

    D156R, R1138A in LbCas12a

    Promoter

    CMV

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMBP-LbCas12a

    Plasmid

    #113431

    Purpose

    Expresses 10xHis-MBP fused to LbCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Has Service

    Cloning Grade DNA

    Insert

    LbCas12a
    

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMBP-FnCas12a

    Plasmid

    #113432

    Purpose

    Expresses 10xHis-MBP fused to FnCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    FnCas12a
    

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Aug. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMBP-AsCas12a

    Plasmid

    #113430

    Purpose

    Expresses 10xHis-MBP fused to AsCas12a

    Depositor

    Jennifer Doudna

    Article

    Chen et al Science. 2018 Apr 27;360(6387):436-439.

    Insert

    AsCas12a
    

    Tags

    MBP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. LbCas12a-crRNA

    Plasmid

    #213753

    Purpose

    For circular RNA-mediated prime editor using LbCas12a-crRNA in HEK293T cells

    Depositor

    Caixia Gao

    Article

    Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

    Insert

    LbCas12a-crRNA
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Feb. 7, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AsdCas12a KRAB

    Plasmid

    #188502

    Purpose

    Expresses FLAG tagged AsdCas12a KRAB

    Depositor

    Isaac Hilton

    Article

    Escobar et al ACS Synth Biol. 2022 Oct 21;11(10):3239-

    Insert

    dCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    D908A

    Promoter

    EF1a

    Available Since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pErCas12a EZ Clone

    Plasmid

    #132641

    Purpose

    Expresses ErCas12a and an sgRNA to target a region of interest

    Depositor

    Stephen Ekker

    Article

    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

    Insert

    ErCas12a
    

    Use

    AAV and CRISPR

    Tags

    3x HA and SV40 NLS

    Expression

    Mammalian

    Mutation

    Q926R (Please see depositor comments) and BsaI si…

    Promoter

    CMV Promoter

    Available Since

    April 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pT3TS nErCas12an

    Plasmid

    #132642

    Purpose

    Used for in vitro transcription of dual NLS ErCas12a mRNA

    Depositor

    Stephen Ekker

    Article

    Wierson et al CRISPR J. 2019 Dec;2(6):417-433. doi: 10

    Insert

    ErCas12a
    

    Use

    CRISPR; In vitro transcription

    Tags

    SV40 NLS

    Promoter

    T3

    Available Since

    Nov. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pPIPL12-FnCas12a

    Plasmid

    #199563

    Purpose

    Tetracycline-Inducible Fncas12a gene expression in E. coli and clostridia

    Depositor

    Philippe Lambin

    Article

    Zhang et al Microbiol Spectr. 2023 Nov 10:e0245923.

    Insert

    Fncas12a
    

    Use

    CRISPR and Synthetic Biology; Tetracycline induci…

    Expression

    Bacterial

    Promoter

    COtetR-PIPL12

    Available Since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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