We narrowed to 263 results for: tet off

1. pCW57.1-FRB-MCU-Flag-TetOFF

   Plasmid

   #185659

   Purpose

   Tet/dox-repressible expression of N-terminus FRB tagged human mitochondrial calcium uniporter with C-terminus Flag epitope

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   Mitochondrial calcium uniporter (MCU Human)
   

   Use

   Lentiviral

   Tags

   FRB and Flag

   Expression

   Mammalian

   Promoter

   pTight TRE

   Available Since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. HYG-TET-OFF – ubiquitin – LEU – 4FLAG

   Plasmid

   #193315

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4FLAG N-terminal tagging
   

   Tags

   4FLAG

   Expression

   Bacterial

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pBabe-Tet-off-puro-mCherry-PA(wt)-Vav2

   Plasmid

   #91873

   Purpose

   Photo-activatable/light sensitive Vav2

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

   Inserts

   LOV2
   VAV2 DH/PH/ZF (Vav2 Mouse)
   

   Tags

   mCherry

   Expression

   Bacterial and Mammalian

   Available Since

   July 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. NAT-TET-OFF – ubiquitin – LEU – 4MYC

   Plasmid

   #193314

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4MYC N-terminal tagging
   

   Tags

   4MYC

   Expression

   Bacterial

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. HYG-TET-OFF – ubiquitin – LEU – 4MYC

   Plasmid

   #193316

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4MYC N-terminal tagging
   

   Tags

   4MYC

   Expression

   Bacterial

   Available Since

   Jan. 26, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-TRE-DIO-eGFP-2A-TetanusToxin (Cre-OFF)

   Plasmid

   #166611

   Purpose

   Encodes Cre-inactivated GFP-2A-Tetanus toxin light chain under control of the TRE

   Depositor

   Martin Myers

   Article

   Sabatini et al eLife. 2021 Mar 11;10. pii: 66835. doi:

   Insert

   EGFP-2A-Tetanus Toxin
   

   Use

   AAV

   Promoter

   tetracycline response element

   Available Since

   April 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Tet-Off-FLEX-DEST (JDW 1186)

   Plasmid

   #229820

   Purpose

   An AAV, tet-off, gateway-compatible destination vector with cre-dependent expression of the insert. EFS driving destablized rTA

   Depositor

   Joshua Wythe

   Article

   Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

   Type

   Empty backbone

   Use

   AAV

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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8. pB-Tet-Off_FLEX_Luc-P2A-H2A-mCherry (JDW 970)

   Plasmid

   #229824

   Purpose

   A PiggyBac vector with a cre-dependent dual luciferase / nuclear mCherry reporter. In the presence of cre, this tet-off vector will be ubiquitously expressed in the absence of dox/tet

   Depositor

   Joshua Wythe

   Article

   Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

   Insert

   Luciferase-P2A-H2A-mCherry
   

   Expression

   Mammalian

   Available Since

   Feb. 26, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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9. KAN(G418)-TET-OFF – ubiquitin – LEU – 4MYC

   Plasmid

   #193318

   Purpose

   Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xMYC N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

   Depositor

   Rob Mitra

   Article

   Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

   Insert

   Ubiquitin - Leucine - 4MYC N-terminal tagging
   

   Tags

   4MYC

   Expression

   Bacterial

   Available Since

   Feb. 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. KAN(G418)-TET-OFF – ubiquitin – LEU – 4FLAG

    Plasmid

    #193317

    Purpose

    Doxycycline-induced transcriptional regulation and Ubiquitin - LEU - 4xFLAG N-terminal tagging. Ubiquitin is cleaved off, resulting in an unstable protein with an N-terminal leucine.

    Depositor

    Rob Mitra

    Article

    Shively et al ACS Synth Biol. 2023 Jan 17. doi: 10.102

    Insert

    Ubiquitin - Leucine - 4FLAG N-terminal tagging
    

    Tags

    4FLAG

    Expression

    Bacterial

    Available Since

    Feb. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pBabe-Tet-off-uniRapR-mCherry-Vav2(DPZ)

    Plasmid

    #91874

    Purpose

    Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

    Insert

    Rapamycin-activatable Vav2;DPZ: DH-PH-ZnF domains of Vav2 (VAV2 Human)
    

    Tags

    mCherry

    Expression

    Bacterial and Mammalian

    Available Since

    Aug. 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tet-off Full length Tau (0N4R, P301L/V337M)

    Plasmid

    #188573

    Purpose

    Dox-repressible expression of the 0N4R Tau isoform

    Depositor

    Franz-Ulrich Hartl

    Article

    Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

    Insert

    0N4R Tau (MAPT Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    P301L and V337M

    Promoter

    pTight TRE

    Available Since

    Feb. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV-Tet-Off-FLEX-Lifeact-mScarlet-HA-WPRE-pA (JDW 721)

    Plasmid

    #229826

    Purpose

    Tet-off, AAV vector for cre dependent expression of Lifeact mScarlet tagged with HA with EFS (minimal EF1a promoter or EF1a short/ EFS) driving destablized tTA (d2tTA)

    Depositor

    Joshua Wythe

    Article

    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

    Insert

    mScarlet-I
    

    Use

    AAV

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pBEST-PTet-MGapt-UTR1-deGFP

    Plasmid

    #227647

    Purpose

    pTet-driven expression of MGapt and deGFP for quantifying cell-free RNA and protein synthesis.

    Depositor

    Richard Murray

    Article

    Kapasiawala et al bioRxiv 2023.03.22.533877

    Insert

    MG aptamer (MGapt) and deGFP
    

    Use

    Synthetic Biology

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. PTet-F30-Pepper aptamer-UTR1-mTurq2

    Plasmid

    #227645

    Purpose

    pTet-driven expression of F30-scaffolded Pepper aptamer and mTurquoise2 for quantifying cell-free RNA and protein synthesis. The mTurq2 has a GGGGS linker followed by a His6x tag.

    Depositor

    Richard Murray

    Article

    Kapasiawala et al bioRxiv 2023.03.22.533877

    Insert

    F30-scaffolded Pepper aptamer and mTurquoise2 (codon optimized for E. coli B using IDT Codon Optimizer)
    

    Use

    Synthetic Biology

    Available Since

    Nov. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPB-TetON-mEGFP-MYOD1_WT

    Plasmid

    #215617

    Purpose

    For integration of MYOD1 WT T2A mEGFP

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    MYOD1 (MYOD1 Human)
    

    Tags

    T2A mEGFP

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pPB-TetON-mEGFP-MYOD1_AroPERFECT-C

    Plasmid

    #215621

    Purpose

    For integration of MYOD1 AroPERFECT C T2A mEGFP

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    MYOD1 AroPERFECT C (MYOD1 Human)
    

    Tags

    T2A mEGFP

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pPB-TetON-mEGFP-MYOD1_AroPERFECT

    Plasmid

    #215620

    Purpose

    For integration of MYOD1 AroPERFECT T2A mEGFP

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    MYOD1 AroPERFECT (MYOD1 Human)
    

    Tags

    T2A mEGFP

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pPB-TetON-mEGFP-MYOD1_AroLITE

    Plasmid

    #215618

    Purpose

    For integration of MYOD1 AroLITE T2A mEGFP

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    MYOD1 AroLITE (MYOD1 Human)
    

    Tags

    T2A mEGFP

    Expression

    Mammalian

    Available Since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pPB-TetON-mEGFP-MYOD1_AroLITE-C

    Plasmid

    #215619

    Purpose

    For integration of MYOD1 AroLITE C T2A mEGFP

    Depositor

    Denes Hnisz

    Article

    Naderi et al Nat Cell Biol. 2024 Aug;26(8):1309-1321.

    Insert

    MYOD1 AroLITE C (MYOD1 Human)
    

    Tags

    T2A mEGFP

    Expression

    Mammalian

    Available Since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge