We narrowed to 398 results for: asns

1. N295A (EcN), E166Q (EcN) MetNI LmH

   Plasmid

   #118269

   Purpose

   N295A and E166Q Mutation of MetN in Methionine ABC Transporter with 6x His Tag. Used to solve crystal structure

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetI (metI Escherichia coli)
   MetN (E166Q ; N295A) (metN Escherichia coli)
   

   Use

   Tags

   6x His Tag

   Expression

   Bacterial

   Mutation

   Changed Glutamic Acid (Glu) 166 to Glutamine (Gln…

   Promoter

   T7 Promoter

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. N229A (EcQ) MetNIQ in pBAD

   Plasmid

   #118257

   Purpose

   N229A Mutation in Methionine Binding Protein (MetQ) and Wild Type Methionine ABC Transporter for Transport Activity Assay

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetN (metN E. coli)
   MetI (metI E. coli)
   MetQ (N229A) (metQ E. coli)
   

   Use

   Tags

   10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

   Expression

   Bacterial

   Mutation

   Changed Asparagine (Asn) 229 to Alanine (Ala) and…

   Promoter

   None and araBAD Promoter

   Available since

   Feb. 27, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. N295A (EcN) MetNIQ in pBAD

   Plasmid

   #118256

   Purpose

   N295A Mutation of MetN in Methionine ABC Transporter and Wild Type Methionine Binding Protein for Transport Activity Assay

   Depositor

   Douglas Rees

   Article

   Nguyen et al Proc Natl Acad Sci U S A. 2018 Oct 23. p

   Inserts

   MetN (N295A) (metN E. coli)
   MetI (metI E. coli)
   MetQ (metQ E. coli)
   

   Use

   Tags

   10x HIs Tag, 6x His Tag, and Enterokinase Cleavag…

   Expression

   Bacterial

   Mutation

   Changed Asparagine (Asn) 295 to Alanine (Ala)

   Promoter

   None and araBAD Promotoer

   Available since

   Nov. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPICZ-His6-K2P4.1A-mCherry-StreptagII

   Plasmid

   #158743

   Purpose

   Expresses K2P4.1 isoform A with TEV protease cleavable N- and C-terminal affinity tags.

   Depositor

   Arthur Laganowsky

   Article

   Schrecke et al Nat Chem Biol. 2021 Jan;17(1):89-95. doi

   Insert

   K2P4.1-A (KCNK4 Human)
   

   Use

   Tags

   His6 and mCherry with Streptag II

   Expression

   Yeast

   Mutation

   Residues 1-264 with N-linked glycosylation sites …

   Promoter

   AOX1

   Available since

   Sept. 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMVtight-RFP-IRES-CD

   Plasmid

   #126678

   Purpose

   Doxycycline inducible expression of CD along with reporter RFP

   Depositor

   Joan Massague

   Article

   Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

   Insert

   RFP-IRES-CD (FCY1 Budding Yeast)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   tight TRE

   Available since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMVtight-UPRT-T2A-RFP-IRES-CD

   Plasmid

   #126677

   Purpose

   Doxycycline inducible expression of UPRT and CD along with reporter RFP

   Depositor

   Joan Massague

   Article

   Basnet et al Elife. 2019 Mar 26;8. pii: 43627. doi: 1

   Insert

   UPRT-T2A-RFP-IRES-CD (FCY1 Toxoplasma gondii, Budding Yeast)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   tight TRE

   Available since

   June 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAR17_StrepI_hFASh_H8_pET22b

   Plasmid

   #122846

   Purpose

   Expresses human type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   human FASN/ human fatty acid synthase (FASN Human)
   

   Use

   Tags

   His8 tag and Twin-Strep tag

   Expression

   Bacterial

   Mutation

   wildtype

   Promoter

   T7 promoter

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAR18_StrepI_mFASm_H8_pET22b

   Plasmid

   #122847

   Purpose

   Expresses murine type I fatty acid synthase (FASN) in Escherichia coli. N-terminal Twin-Strep tag; C-terminal H8-tag; in pET22b vector.

   Depositor

   Martin Grininger

   Article

   Rittner et al Protein Sci. 2019 Feb;28(2):414-428. doi

   Insert

   mouse FASN/ mouse fatty acid synthase (Fasn Mouse)
   

   Use

   Tags

   His8 tag and Twin-Strep tag

   Expression

   Bacterial

   Mutation

   wildtype

   Promoter

   T7 promoter

   Available since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRSF-T20S

   Plasmid

   #110805

   Purpose

   expresses Thermoplasma acidophilium 20S proteasome in E. Coli

   Depositor

   Yifan Cheng

   Article

   Yu et al Nat Commun. 2020 Sep 11;11(1):4580. doi:

   Inserts

   PsmB
   PsmA
   

   Use

   Tags

   tev-His

   Expression

   Bacterial

   Mutation

   N2D and Q2E

   Promoter

   T7 and T7 promoter

   Available since

   July 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pB-T-PAF-Clu CC1

    Plasmid

    #215386

    Purpose

    High expression of the Clusterin(p.Asn239_Phe262del) mutant for purification.

    Depositor

    Franz-Ulrich Hartl

    Insert

    CLU (CLU Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    p.Asn239_Phe262del

    Promoter

    tetO

    Available since

    May 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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