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We narrowed to 501 results for: SPY

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pTKA001

Plasmid

#164577

Purpose

Expresses SARS-CoV-2 nsp7 from a pET46 backbone for IPTG-inducible bacterial expression

Depositor

Robert Kirchdoerfer

Article

Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

Insert

nsp7 (ORF1ab )

Use

Tags

6xHis, Ek, TEV

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Jan. 25, 2021

Availability

Academic Institutions and Nonprofits only

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pTKA002

Plasmid

#164578

Purpose

Expresses SARS-CoV-2 nsp8 from a pET46 backbone for IPTG-inducible bacterial expression

Depositor

Robert Kirchdoerfer

Article

Altincekic et al Front Mol Biosci. 2021 May 10;8:653148.

Insert

nsp8

Use

Tags

6xHis, Ek, TEV

Expression

Bacterial

Mutation

Promoter

T7 Promoter

Available since

Feb. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pQE80l AEW

Plasmid

#196481

Purpose

Protein expression of SpyTag-ELP-WSCP

Depositor

Fei Sun

Insert

AEW

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

March 13, 2023

Availability

Academic Institutions and Nonprofits only

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pBS mFgfr1 (CT#92)

Plasmid

#14005

Depositor

Cliff Tabin

Insert

Fgfr1 (Fgfr1 Mouse)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Feb. 9, 2007

Availability

Academic Institutions and Nonprofits only
RCAS(A) mFgfr1 DN (CT#984)

Plasmid

#14006

Depositor

Cliff Tabin

Insert

Fgfr1 (Fgfr1 Mouse)

Use

Retroviral; Avian expression

Tags

Expression

Mutation

Dominant negative

Promoter

Available since

Feb. 16, 2007

Availability

Academic Institutions and Nonprofits only

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pGEXndoS

Plasmid

#44655

Depositor

Mattias Collin

Article

Collin et al Infect Immun. 2001 Nov . 69(11):7187-9.

Insert

ndoS (ndoS Streptococcus pyogenes)

Use

Tags

Gst

Expression

Bacterial

Mutation

None

Promoter

tac

Available since

May 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW092

Plasmid

#113250

Purpose

E. coli expression vector for His-MBP-S. pyogenes Cas1 H205A

Depositor

Jennifer Doudna

Article

Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

Insert

S. pyogenes Cas1

Use

Tags

6xHis-MBP

Expression

Bacterial

Mutation

H205A

Promoter

T7

Available since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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Halo-TMD

Plasmid

#195347

Purpose

Fluorescent labeling for cell membranes

Depositor

Allen Liu

Article

Hsu et al ACS Sens. 2023 Jan 27;8(1):12-18. doi: 1

Insert

HaloTag

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pKDC.109

Plasmid

#227189

Purpose

T7 IPTG-driven Cas9

Depositor

Seth Shipman

Article

Fishman et al Nat Biotechnol. 2024 Sep 5. doi: 10.1038

Insert

SpyCas9

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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4xNLS-pMJ915v2

Plasmid

#88917

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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4xNLS-pMJ915v2-sfGFP

Plasmid

#88921

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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2xNLS-pMJ915v2

Plasmid

#88916

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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1xNLS-pMJ915v2

Plasmid

#88915

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Insert

Cas9

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pMJ915v2 + sfGFP

Plasmid

#88918

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 2, 2017

Availability

Academic Institutions and Nonprofits only

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pQE80L TriCatcher-ELP

Plasmid

#112630

Purpose

Two SpyCatchers linked by an elastin-like polypeptide with a C-terminal SnoopCatcher

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

TriCatcher-ELP

Use

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

Promoter

T5

Available since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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sgRNA AAVS1

Plasmid

#128119

Purpose

Co-expresses sgRNA AAVS1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA AAVS1 + GFP

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

RSV/U6

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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7xNLS-pMJ915v2-sfGFP

Plasmid

#88922

Purpose

For expression of modified SpyCas9 in e.coli.

Depositor

Jennifer Doudna

Article

Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

Inserts

Cas9
T7

Use

CRISPR

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 4, 2017

Availability

Academic Institutions and Nonprofits only

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pGEXndoS(E235Q)

Plasmid

#44656

Depositor

Mattias Collin

Article

Allhorn et al BMC Microbiol. 2008 . 8():3.

Insert

ndoS (ndoS Streptococcus pyogenes)

Use

Tags

Gst

Expression

Bacterial

Mutation

Changed Glutamic acid 235 to Glutamine

Promoter

tac

Available since

May 24, 2013

Availability

Academic Institutions and Nonprofits only

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Cas-Acr (insertion 5)

Plasmid

#128116

Purpose

Expresses SpyCas9 fused to attenuated AcrIIA4 (insertion 5)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (insertion 5)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Cas-Acr (N39A)

Plasmid

#128117

Purpose

Expresses SpyCas9 fused to attenuated AcrIIA4 (N39A)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

CascAID (N39A)

Use

CRISPR and Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge