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Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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We narrowed to 9,480 results for: Coli

Showing: 381 - 400 of 9480 results

  1. pCH6 - pBait-ChiX

    Plasmid
    #174662
    Purpose
    arabinose-induced synthesis of a hybrid RNA containing a single MS2 hairpin followed by E coli ChiX inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.
    Depositor
    Katherine Berry
    Article
    Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507
    Insert
    ChiX
    Use
    Tags
    1xMS2 hairpin
    Expression
    Bacterial
    Mutation
    Promoter
    pBad
    Available since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174662-plasmid-map-sequence-id-361921

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  2. pGBW-m4046810

    Plasmid
    #149251
    Purpose
    Bacterial expression plasmid for SARS-CoV-2 N (nucleocapsid)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Insert
    SARS-CoV-2 N (nucleocapsid) (N Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    Cleavable TEV;HA
    Expression
    Bacterial
    Mutation
    Escherichia coli recode 1
    Promoter
    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…
    Available since
    May 11, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    149251-plasmid-map-sequence-id-357431

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  12. pmr101A-cPr-mCherry (KmR)

    Plasmid
    #177207
    Purpose
    Plasmid with p15A origin for constitutive mCherry expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic constitutive promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177207-plasmid-map-sequence-id-348603

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  13. EcMscL

    Plasmid
    #92418
    Purpose
    E. coli Mechanosensitive Channel of Large Conductance
    Depositor
    Douglas Rees
    Insert
    MscL (mscL E. coli)
    Use
    Tags
    6x Histidine Tag and Thrombin Cleavage Site betwe…
    Expression
    Bacterial
    Mutation
    Promoter
    T7 Promoter
    Available since
    July 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    92418-plasmid-map-sequence-id-183401

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  14. p416-GPD-HA-PPK

    Plasmid
    #183943
    Purpose
    Expresses HA-tagged E. coli PPK from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    PPK (ppk E. coli)
    Use
    Tags
    HA
    Expression
    Yeast
    Mutation
    WT
    Promoter
    GPD
    Available since
    June 2, 2022
    Availability
    Academic Institutions and Nonprofits only
    183943-plasmid-map-sequence-id-363305

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  15. pmr101A-cPr-mCherry (ErmR)

    Plasmid
    #177206
    Purpose
    Plasmid with p15A origin for constitutive mCherry expression in E. coli
    Depositor
    Alexey Merz
    Insert
    mCherry
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Synthetic constitutive promoter
    Available since
    Nov. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    177206-plasmid-map-sequence-id-348606

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  16. pAIP4_1TJE

    Plasmid
    #234203
    Purpose
    Heterologous protein expression of threonine--tRNA ligase (EC 6.1.1.3) (Threonyl-tRNA synthetase) in Escherichia coli
    Depositor
    Christopher Bahl
    Insert
    1TJE
    Use
    Tags
    10x HisTag-Smt3
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Industry, Academic Institutions, and Nonprofits
    234203-plasmid-map-sequence-id-464168

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  17. p416-GPD-PPK(mutant)

    Plasmid
    #183942
    Purpose
    Expresses catalytic mutant of E. coli PPK from yeast GPD promoter
    Depositor
    Michael Downey
    Insert
    PPK(mutant) (ppk E. coli )
    Use
    Tags
    Expression
    Yeast
    Mutation
    mutations in His435, His454, and His592 to Alanine
    Promoter
    GPD
    Available since
    June 3, 2022
    Availability
    Academic Institutions and Nonprofits only
    183942-plasmid-map-sequence-id-363372

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  18. pJK649

    Plasmid
    #72399
    Purpose
    Produces Escherichia coli phosphoribosylpyrophosphate amidotransferase (EcPurF)
    Depositor
    T. J. Kappock
    Insert
    amidophosphoribosyltransferase
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    T7
    Available since
    Feb. 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    72399-plasmid-map-sequence-id-133993

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  19. pCASCADE-zwf

    Plasmid
    #65825
    Purpose
    zwf targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    zwf guide
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induc…
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    65825-plasmid-map-sequence-id-124656

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  20. pCASCADE-udhA

    Plasmid
    #65818
    Purpose
    udhA targeting guide RNA E. coli , Low Phosphate Induction
    Depositor
    Michael Lynch
    Insert
    udhA guide
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    E . coli ugpB gene promoter, low phosphate induc…
    Available since
    Aug. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    65818-plasmid-map-sequence-id-111976

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Showing: 381 - 400 of 9480 results