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We narrowed to 767 results for: EB1

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Showing: 381 - 400 of 767 results

  1. pUBC_HA_2xPP7-VP64

    Plasmid
    #68418
    Purpose
    Transient expression of two tandem copies of the non-aggregating delta-FG mutant, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    PP7(deltaFG)
    Use
    Synthetic Biology
    Tags
    HA and VP64
    Expression
    Mammalian
    Mutation
    PP7: [delta]67_74 (non-aggregating)
    Promoter
    human UbC
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68418-plasmid-map-sequence-id-125550

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  2. pUBC_HA_PP7-mCherry

    Plasmid
    #107605
    Purpose
    Transient expression of non-aggregating delta-FG mutant, fused to mCherry in mammalian cells, under a hUBC promoter, HA tag
    Depositor
    John Rinn
    Publication
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    PP7-mCherry
    Use
    Synthetic Biology
    Tags
    mCherry
    Mutation
    PP7: [delta]67_74 (non-aggregating)
    Promoter
    hUBC
    Available since
    April 23, 2018
    Availability
    Academic Institutions and Nonprofits only
    107605-plasmid-map-sequence-id-207484

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  6. pEF_dCas9-VP64

    Plasmid
    #68417
    Purpose
    Transient expression of Sp-dCas9 fused to the VP64 transcription activator, in mammalian cells, under an EF1-alpha promoter.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Use
    CRISPR
    Tags
    3xFLAG and VP64
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    human EF1[alpha]
    Available since
    Sept. 18, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    68417-plasmid-map-sequence-id-120195

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  7. pUBC_hsPUM1-iRFP670

    Plasmid
    #107609
    Purpose
    Transient expression of Pumilio1, fused to iRFP670 in mammalian cells, under a hUBC promoter
    Depositor
    John Rinn
    Publication
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    hsPUM1-iRFP670
    Use
    Synthetic Biology
    Tags
    iRFP670
    Expression
    Mammalian
    Promoter
    human UBC
    Available since
    April 30, 2018
    Availability
    Academic Institutions and Nonprofits only
    107609-plasmid-map-sequence-id-207515

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  8. HUWE1-sgRNA-2

    Plasmid
    #86925
    Purpose
    To express sgRNA target HUWE1 gene
    Depositor
    Yihong Ye
    Publication
    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HUWE1 sgRNA2 (HUWE1 Human)
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 7, 2017
    Availability
    Academic Institutions and Nonprofits only

  9. HUWE1-sgRNA-1

    Plasmid
    #86924
    Purpose
    To express sgRNA target HUWE1 gene
    Depositor
    Yihong Ye
    Publication
    Xu et al Cell Discov. 2016 Nov 8;2:16040. eCollec
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    HUWE1 sgRNA1 (HUWE1 Human)
    Expression
    Mammalian
    Promoter
    U6
    Available since
    April 6, 2017
    Availability
    Academic Institutions and Nonprofits only

  10. pTrans_9xD3B_Cluc_Norm

    Plasmid
    #68415
    Purpose
    CRISPR-Display Cypridina Luciferase-2A-Venus YFP "normalizer," transient format.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Cypridina Luciferase-2A-Venus YFP
    Use
    Luciferase
    Expression
    Mammalian
    Mutation
    YFP: M153T,V163A,S175G ("Venus") YFP Va…
    Promoter
    minimalCMV
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    68415-plasmid-map-sequence-id-125547

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  14. pU6_(GLuc)_INT[Spinach2]

    Plasmid
    #68430
    Purpose
    Transient expression in mammalian cells of an "INT" construct_bearing the "Spinach2" aptamer, targeting the GLuc reporter. U6 promoter
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Insert
    INT construct_bearing the "Spinach2" aptamer
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U6
    Available since
    Oct. 5, 2015
    Availability
    Academic Institutions and Nonprofits only
    68430-plasmid-map-sequence-id-120208

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  18. pUBC_V5_MS2-mVenus

    Plasmid
    #107606
    Purpose
    Transient expression of humanized MS2 (V75E/A81G), fused to mVenus in mammalian cells, under a hUBC promoter, V5 tag
    Depositor
    John Rinn
    Publication
    Maass et al Nat Struct Mol Biol. 2018 Jan 8. pii: 10
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    MS2-mVenus
    Use
    Synthetic Biology
    Tags
    V5 and mVenus
    Mutation
    MS2: V75E/A81G
    Promoter
    human UBC
    Available since
    May 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    107606-plasmid-map-sequence-id-209713

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  19. pUBC_HA_L7Ae-VP64

    Plasmid
    #68421
    Purpose
    Transient expression of humanized A. fulgidus L7Ae, fused to the VP64 transcription activator, in mammalian cells, under a UBC promoter
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    L7Ae
    Use
    Synthetic Biology
    Tags
    HA and VP64
    Expression
    Mammalian
    Promoter
    human UbC
    Available since
    Sept. 21, 2015
    Availability
    Academic Institutions and Nonprofits only
    68421-plasmid-map-sequence-id-124596

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  20. pU1/Sm/3' Box_(GLuc)_TOP1

    Plasmid
    #68437
    Purpose
    Transient expression of the "TOP1" construct, targeting the GLuc reporter, in mammalian cells. U1Pro/SmBox/3' Box expression backbone.
    Depositor
    John Rinn
    Publication
    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    TOP1 construct
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Promoter
    human U1
    Available since
    Sept. 22, 2015
    Availability
    Academic Institutions and Nonprofits only
    68437-plasmid-map-sequence-id-124681

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Showing: 381 - 400 of 767 results