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Plasmid#167827PurposeControl sensor for EKAREN4DepositorInsertEKAREN4(TA)
UseTagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN5(TA) + PGK-blast
Plasmid#167820Purposecontrol sensor for EKAREN5DepositorInsertEKAREN5(TA)
UseTagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPHR-mCherry-hHP1β
Plasmid#103830PurposeSoluble BLInCR effector that is recruited to 'localizer' sites upon blue light illuminationDepositorUseTagsExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN4 + PGK-puro
Plasmid#167824PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN4
UseTagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLV x EF1a_EKAREN4(TA)-ires-puro
Plasmid#167830PurposeControl sensor for EKAREN4DepositorInsertEKAREN4(TA)
UseLentiviralTagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN4(TA) + PGK-puro
Plasmid#167826PurposeControl sensor for EKAREN4DepositorInsertEKAREN4(TA)
UseTagsnls localization motifExpressionMammalianMutationK424P, K426W, T420APromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
miniTol2 x EF1a_EKAREN4 + PGK-blast
Plasmid#167825PurposeOptimized EKAREV FRET biosensor sensor for ERKDepositorInsertEKAREN4
UseTagsnls localization motifExpressionMammalianMutationK424P, K426WPromoterEF1aAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV1.2 WPRE
Plasmid#236241PurposeLentiviral expression of voltage-gated calcium channel, CaV1.2, N-terminally tagged with mEmeraldDepositorInsertCaV1.2 (CACNA1C Rabbit)
UseLentiviralTagsmEmeraldExpressionMutationPromoterhuman Synapsin 1Available sinceMay 5, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.1 WPRE
Plasmid#236239PurposeLentiviral expression of voltage-gated calcium channel, CaV2.1, N-terminally tagged with mEmeraldDepositorInsertCaV2.1 (CACNA1A Rabbit)
UseLentiviralTagsmEmeraldExpressionMutationPromoterhuman Synapsin 1Available sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON mEmerald-ER WPRE
Plasmid#236230PurposeAAV expression of a fluorescent marker, mEmerald fused to Prolactin signal peptide and KDEL sequence; for expression and retention in the lumen of the endoplasmic reticulumDepositorInsertmEmerald-PRL signal peptide-KDEL sequence (PRL Bovine)
UseAAVTagsmEmeraldExpressionMutationPromoterhuman Synapsin 1Available sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON HaloTag-Sec61b WPRE
Plasmid#236229PurposeAAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to HaloTagDepositorInsertHaloTag-Sec61b (SEC61B Human, Synthetic)
UseAAVTagsHaloTagExpressionMutationPromoterhuman Synapsin 1Available sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON mCherry-Jph3 WPRE
Plasmid#236237PurposeAAV expression of human Junctophilin3 N-terminally tagged with mCherryDepositorInsertJunctophilin 3 (JPH3 Human)
UseAAVTagsmCherryExpressionMutationPromoterhuman Synapsin 1Available sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV KI Cloning Vector_hSyn mTagBFP2-CAAX2
Plasmid#236245PurposeCloning template for knock-in based strategy with U6 promoter, gRNA and donor tag cloning cassettes, and a fluorescent marker for the plasma membrane under hSyn promoterDepositorInsertmTagBFP2 fused to the plasma membrane targeting sequence CAAX2
UseAAVTagsmTagBFP2ExpressionMutationPromoterU6 and human Synapsin1Available sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pLenti_SIV-(SalI)-syn-IRES-mEmerald-CaV2.2 WPRE
Plasmid#236240PurposeLentiviral expression of voltage-gated calcium channel, CaV2.2, N-terminally tagged with mEmeraldDepositorInsertCaV2.2 (CACNA1B Rabbit)
UseLentiviralTagsmEmeraldExpressionMutationPromoterhuman Synapsin 1Available sinceMay 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
pAAV hSyn INTRON mEmerald-Sec61b WPRE
Plasmid#236228PurposeAAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to mEmerald.DepositorInsertmEmerald-Sec61b (SEC61B Human, Synthetic)
UseAAVTagsmEmeraldExpressionMutationPromoterhuman Synapsin 1Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pGFP-nE-mHP1alpha
Plasmid#181895PurposeFluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamatesDepositorInsertnE-mHP1alpha (Cbx5 Mouse)
UseTagsGFPExpressionMammalianMutationS11E, S12E, S13E, S14EPromoterCMVAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-nE-mHP1alpha-delCTE
Plasmid#181896PurposeFluorescently tagged HP1alpha, serines in N-terminal extension (NTE) replaced by glutamates, C-terminal deletionDepositorInsertnE-mHP1alpha-delCTE (Cbx5 Mouse)
UseTagsGFPExpressionMammalianMutationS11E, S12E, S13E, S14E, 14 aa C-terminal deletionPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-FUSN-mHP1alpha
Plasmid#181897PurposeFluorescently tagged HP1alpha fused to the N-terminal domain of the fused in sarcoma protein (FUSN)DepositorInsertFUSN-mHP1alpha (Cbx5 Mouse, Human)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGFP-PGL-1-mHP1alpha
Plasmid#181899PurposeFluorescently tagged HP1alpha fused to PGL-1DepositorInsertPGL-1-mHP1alpha (Cbx5 Nematode, Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pRGG-GFP-RGG-mHP1alpha
Plasmid#181898PurposeFluorescently tagged HP1alpha fused to the arginine/glycine-rich RGG domain of LAF-1DepositorInsertRGG-GFP-RGG (Cbx5 Nematode, Mouse)
UseTagsGFPExpressionMammalianMutationPromoterCMVAvailable sinceApril 20, 2022AvailabilityAcademic Institutions and Nonprofits only