We narrowed to 11,460 results for: NSI

1. pET-Cb2

   Plasmid

   #53529

   Purpose

   Expresses an insect mitochondrial arginyl-tRNA synthetase in E.coli

   Depositor

   Gabor Igloi

   Article

   Leisinger et al Insect Biochem Mol Biol. 2013 Dec;43(12)

   Insert

   mitochondrial arginyl-tRNA synthetase
   

   Use

   Tags

   His Tag and Thioredoxin

   Expression

   Bacterial

   Mutation

   changed Ser4 to Ala4 to permit NcoI cloning

   Promoter

   T7

   Available since

   July 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pIRES:hTRAAK(W262S)

   Plasmid

   #133078

   Purpose

   Mammalian expression vector. It will generate the human TRAAK channel full lenght W262S mutant

   Depositor

   Dan Minor

   Article

   Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

   Insert

   Potassium channel subfamily K member 4 (KCNK4, TRAAK) W262S (KCNK4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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3. pIRES:hTRAAK(G124I)

   Plasmid

   #133079

   Purpose

   Mammalian expression vector. It will generate the human TRAAK channel full lenght G124I mutant

   Depositor

   Dan Minor

   Article

   Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

   Insert

   Potassium channel subfamily K member 4 (KCNK4, TRAAK) G124I (KCNK4 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   G124I

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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4. pPICZ:hTRAAK(W262S)

   Plasmid

   #130670

   Purpose

   P. pastoris expression vector. It will generate the human TRAAK channel (1-300) fused to a C-terminal GFP

   Depositor

   Dan Minor

   Article

   Lolicato et al Neuron. 2014 Dec 17;84(6):1198-212. doi:

   Insert

   KCNK4 (KCNK4 Human)
   

   Use

   Tags

   SNS- 3C_cleavage_site-TAAA-GFP

   Expression

   Yeast

   Mutation

   N104Q, N108Q, W262S

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGreenIIM RPS5A-mDII-ntdTomato/RPS5A-DII-n3Venus

   Plasmid

   #61629

   Purpose

   R2D2: Expresses a fusion of auxin-sensitive DII to nuclear 3xVenus and auxin-insensitive mDII to nuclear tdTomato, both from the Arabidopsis RPS5A promoter

   Depositor

   Dolf Weijers

   Article

   Liao et al Nat Methods. 2015 Mar;12(3):207-10. doi:

   Inserts

   mDII
   ntdTomato
   DII
   n3xVenus
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   RPS5A

   Available since

   March 5, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. Flag-Bcl2

   Plasmid

   #18003

   Depositor

   Clark Distelhorst

   Article

   Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

   Insert

   Bcl-2 (BCL2 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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7. pLenti-PGK-Venus-Akaluc (neo)

   Plasmid

   #124701

   Purpose

   Aka-Luciferase reporter

   Depositor

   Roland Friedel

   Article

   Bozec et al Neurooncol Adv. 2020 Oct 10;2(1):vdaa134

   Insert

   Venus-Akaluciferase
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   hPGK

   Available since

   Aug. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBABE HA Chk2 WT

   Plasmid

   #41901

   Depositor

   Stephen Elledge

   Article

   Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

   Insert

   CHK2 (CHEK2 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 15, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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9. GFP-Bcl2

   Plasmid

   #17999

   Depositor

   Clark Distelhorst

   Article

   Wang et al J Biol Chem. 2001 Nov 23. 276(47):44117-

   Insert

   Bcl-2 (BCL2 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 4, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE/VEGF/IRES/EGFP

    Plasmid

    #206211

    Purpose

    An expression vector of VEGF and EGFP under control of TRE promoter

    Depositor

    Masamichi Kamihira

    Article

    Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

    Insert

    vascular endothelial growth factor (Vegfa Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. FUW-tetO-EGFP

    Plasmid

    #84041

    Purpose

    3rd generation lentiviral transfer plasmid. Expresses EGFP and puromycin resistance in mammalian cells under the control of a doxycycline-inducible promoter

    Depositor

    Stefano Piccolo

    Article

    Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

    Insert

    Enhanced Green Fluorescence Protein, Puromycin resistance gene
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE-CMV

    Available since

    Oct. 31, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAS_4xMmaPylT(UUA)_EF1_MmaPylRS

    Plasmid

    #174896

    Purpose

    Mma PylRS expression for ochre suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAS_4xMmaPylT(UCA)_EF1_MmaPylRS

    Plasmid

    #174899

    Purpose

    Mma PylRS expression for opal suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

    Plasmid

    #140018

    Purpose

    Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    EcoTyrRS
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y37L, D182S, F183M, L186A

    Promoter

    EF1

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. HSV-DYN-hM4Di

    Plasmid

    #53327

    Purpose

    Dynorphin promoter driving hM4Di

    Depositor

    John Neumaier

    Article

    Ferguson et al Nat Neurosci. 2011 Jan;14(1):22-4. doi:

    Insert

    hM4Di (Pdyn Rat)
    

    Use

    Hsv

    Tags

    3xHA tag

    Expression

    Mammalian

    Mutation

    Promoter

    Rat dynorphin promoter ( -1858 to +135)

    Available since

    May 11, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBAD-bHs

    Plasmid

    #134259

    Purpose

    For L-arabinose inducible bacterial expression of S. typhimurium codon-optimized hyaluronidase (Streptomyces koganeinsis UniProt A0A0U2E2) with N-terminal 6x His tag

    Depositor

    Edwin Manuel

    Article

    Ebelt et al Mol Cancer Ther. 2019 Nov 6. pii: 1535-7

    Insert

    Hyaluronidase from Streptomyces koganeinsis codon-optimized for Salmonella typhimurium
    

    Use

    Tags

    6x His

    Expression

    Bacterial

    Mutation

    codon optimized for Salmonella typhimurium

    Promoter

    AraC

    Available since

    Nov. 26, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGM190

    Plasmid

    #69614

    Purpose

    inducible gene expression in Streptomyces

    Depositor

    Günther Muth

    Article

    Reuther et al Mol Microbiol. 2006 Jul;61(2):436-46. do

    Inserts

    aphII
    tsr
    rep
    sso
    TipA promoter
    dso
    

    Use

    Streptomyces- e. coli shuttle vector

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP-alpha-E-Catenin 3xFP4 (895)

    Plasmid

    #67936

    Purpose

    Mammalian expression of alpha E catenin

    Depositor

    Alpha Yap

    Article

    Leerberg et al Curr Biol. 2014 Aug 4;24(15):1689-99. do

    Insert

    alpha E catenin (Ctnna1 Mouse)
    

    Use

    Tags

    3xFP4 and EGFP

    Expression

    Mammalian

    Mutation

    N-terminal domain

    Promoter

    CMV

    Available since

    Oct. 21, 2015

    Availability

    Academic Institutions and Nonprofits only

19. MDH1-shSHP2-3-H2Kb2

    Plasmid

    #17854

    Depositor

    Chang-Zheng Chen

    Article

    Li et al Cell. 2007 Apr 6. 129(1):147-61.

    Insert

    shSHP2 (PTPN11 Human)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 16, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBabe-HA-Chk2-D311V

    Plasmid

    #41921

    Depositor

    Stephen Elledge

    Article

    Matsuoka et al Science. 2007 May 25;316(5828):1160-6.

    Insert

    Chk2 (CHEK2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    D311V

    Promoter

    Available since

    April 18, 2013

    Availability

    Academic Institutions and Nonprofits only