Addgene

We narrowed to 2,116 results for: PAM

Showing: 381 - 400 of 2116 results

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pGFP-GTATT

Plasmid

#170099

Purpose

deGFP reporter plasmid with GTATT PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

p70a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pGFP-ATAAC

Plasmid

#170095

Purpose

deGFP reporter plasmid with ATAAC PAM upstream of p70a promoter

Depositor

Chase Beisel

Article

Wimmer et al Methods Mol Biol. 2022;2433:391-411. doi

Insert

deGFP

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

p70a

Available since

Jan. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pOLENTE

Plasmid

#80683

Purpose

DEST vector for cell-free in vitro expression

Depositor

Nico Dissmeyer

Type

Empty backbone

Use

Synthetic Biology; In vitro transcription and tra…

Tags

Expression

Mammalian

Mutation

Promoter

T7 and SP6

Available since

Aug. 16, 2016

Availability

Academic Institutions and Nonprofits only

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WN10150

Plasmid

#80443

Purpose

Expresses dead (908A) AsCpf1

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

nuclease dead (908A) AsCpf1

Use

Tags

NLS-3xHA

Expression

Mammalian

Mutation

nuclease dead (D908A)

Promoter

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

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pTE4938

Plasmid

#107527

Purpose

Expresses Fn crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Fn crRNA
mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CBh and human U6

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

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pTE4495

Plasmid

#80338

Purpose

mammalian expression MbCpf1 nuclease and Mb crRNA

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Mb crRNA
MbCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

CMV and human U6

Available since

Aug. 19, 2016

Availability

Academic Institutions and Nonprofits only

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pTE4560

Plasmid

#107526

Purpose

Expresses As crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

As crRNA
mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CBh and human U6

Available since

April 4, 2018

Availability

Academic Institutions and Nonprofits only

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pTE4999

Plasmid

#107555

Purpose

Expresses human codon optimized inactive FnCpf1(dead1) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hFnCpf1(dead1)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

D917A

Promoter

CMV

Available since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pTE4565

Plasmid

#88903

Purpose

Expresses inactive/dead, humanized MbCpf1 nuclease

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hMbCpf1(D986A)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTE4567

Plasmid

#107557

Purpose

Expresses human codon optimized inactive MbCpf1(dead2) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hMbCpf1(dead2)

Use

Tags

3xHA and NLS

Expression

Mammalian

Mutation

E1080A

Promoter

CMV

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTE4561

Plasmid

#107525

Purpose

Expresses Lb crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Lb crRNA
mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CBh and human U6

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTE3320

Plasmid

#107529

Purpose

Expresses human codon optimized LbCpf1(RVR mutant) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hLbCpf1(RVR)

Use

Tags

3xHA and NLS

Expression

Mammalian

Mutation

G532R, K538V, Y542R

Promoter

CMV

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTE3314

Plasmid

#107530

Purpose

Expresses human codon optimized FnCpf1(RVR mutant) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hFnCpf1(RVR)

Use

Tags

3xHA and NLS

Expression

Mammalian

Mutation

N607R, K613V, N617R

Promoter

CMV

Available since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pTE3319

Plasmid

#107533

Purpose

Expresses human codon optimized MbCpf1(RR mutant) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hMbCpf1(RR)

Use

Tags

3xHA and NLS

Expression

Mammalian

Mutation

N576R, K637R

Promoter

CMV

Available since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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pTE3316

Plasmid

#107532

Purpose

Expresses human codon optimized FnCpf1(RR mutant) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hFnCpf1(RR)

Use

Tags

3xHA and NLS

Expression

Mammalian

Mutation

N607R, K671R

Promoter

CMV

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTE3317

Plasmid

#107531

Purpose

Expresses human codon optimized MbCpf1(RVR mutant) in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Insert

hMbCpf1(RVR)

Use

Tags

3xHA and NLS

Expression

Mammalian

Mutation

N576R, K582V, N586R

Promoter

CMV

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pTE3179

Plasmid

#107528

Purpose

Expresses Mb crRNA and mCherry in mammalian cells.

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Mb crRNA
mCherry

Use

Tags

Expression

Mammalian

Mutation

Promoter

CBh and human U6

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE4497

Plasmid

#80339

Purpose

mammalian expression FnCpf1 nuclease and Fn crRNA

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Fn crRNA
FnCpf1

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

CMV and human U6

Available since

Aug. 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE4889

Plasmid

#88905

Purpose

Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Fn crRNA
hFnCpf1(D917A)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

CMV and human U6

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pTE4566

Plasmid

#88904

Purpose

Expresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nuclease

Depositor

Ervin Welker

Article

Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

Inserts

Mb crRNA
hMbCpf1(D986A)

Use

CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Mutation

Promoter

CMV and human U6

Available since

April 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge