We narrowed to 391 results for: Tight TRE

1. pCW57.1-FRB-MCU-Flag-TetOFF

   Plasmid

   #185659

   Purpose

   Tet/dox-repressible expression of N-terminus FRB tagged human mitochondrial calcium uniporter with C-terminus Flag epitope

   Depositor

   Dipayan Chaudhuri

   Article

   Balderas et al Nat Commun. 2022 May 19;13(1):2769. doi:

   Insert

   Mitochondrial calcium uniporter (MCU Human)
   

   Use

   Lentiviral

   Tags

   FRB and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   pTight TRE

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. Tet-off Full length Tau (0N4R, P301L/V337M)

   Plasmid

   #188573

   Purpose

   Dox-repressible expression of the 0N4R Tau isoform

   Depositor

   Franz-Ulrich Hartl

   Article

   Saha et al Nat Commun. 2023 Feb 2;14(1):560. doi: 1

   Insert

   0N4R Tau (MAPT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   P301L and V337M

   Promoter

   pTight TRE

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB-C6F/TdTomato

   Plasmid

   #140826

   Purpose

   OCT4, SOX2, KLF4, C-MYC, KLF2, NANOG (C6F) and tdTomato under the control of TRE (Tet-ON)

   Depositor

   Hideyuki Okano

   Article

   Kisa et al Stem Cell Reports. 2017 Dec 12;9(6):1825

   Inserts

   OCT4 (POU5F1 Human)
   SOX2 (SOX2 Human)
   KLF4 (KLF4 Human)
   C-MYC (MYC Human)
   KLF2 (KLF2 Human)
   NANOG (NANOG Human)
   tdTomato
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L104P- please see depositor comments

   Promoter

   Available since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCW57.1-Tet-UPF1WT (pRKB264)

   Plasmid

   #99146

   Purpose

   Inducible lentiviral construct to express FLAG-tagged UPF1wt

   Depositor

   Robert Bradley

   Article

   Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

   Insert

   UPF1 (UPF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   First 127 codons are optimized to reduce GC conte…

   Promoter

   pTight

   Available since

   Nov. 27, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCW57.1-Tet-UPF1S124A/N138A/T139A (pRKB268)

   Plasmid

   #99144

   Purpose

   Inducible lentiviral construct to express FLAG-tagged UPF1S124A/N138A/T139A

   Depositor

   Robert Bradley

   Article

   Feng et al Mol Cell. 2017 Jul 20;67(2):239-251.e6.

   Insert

   UPF1 (UPF1 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   First 127 codons are optimized to reduce GC conte…

   Promoter

   pTight

   Available since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-syn-FLEX-splitTVA-EGFP-tTA

   Plasmid

   #100798

   Purpose

   helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G

   Depositor

   Ian Wickersham

   Article

   Liu et al Nature. 2017 Aug 31;548(7669):582-587. d

   Has Service

   AAV1

   Insert

   TVA, EGFP, tet transactivator
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   synapsin

   Available since

   Oct. 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-syn-F14F15S-splitTVA-EGFP-tTAv2

   Plasmid

   #183352

   Purpose

   Flp-dependent helper virus for monosynaptic tracing; to be coinjected with pAAV-TREtight-mTagBFP2-B19G (Addgene #100799)

   Depositor

   Ian Wickersham

   Article

   Jin et al Nat Neurosci. 2024 Jan 11. doi: 10.1038/

   Insert

   TVA, EGFP and tTA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. AAVS1 KanR foundation lox casette

   Plasmid

   #132388

   Purpose

   Foundation cassette containing lox sites for cre-mediated exchange of inducible vectors, confers mammalian G418 resistance, targets AAVS1 locus

   Depositor

   Gerold Schmitt-Ulms

   Article

   Wang et al Bio Protoc. 2020 May 5;10(9):e3615. doi:

   Insert

   G418 resistance
   

   Use

   CRISPR and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   promoterless

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. TERT - R865C

   Plasmid

   #213926

   Purpose

   Expresses Mutant TERT R865C

   Depositor

   Coleman Lindsley

   Article

   Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

   Insert

   Mutant TERT R865C (TERT Human)
   

   Use

   Lentiviral

   Tags

   6x His Tag

   Expression

   Mammalian

   Mutation

   Arginine 865 changed to Cysteine

   Promoter

   Tet-responsive promoter PTight, consisting of sev…

   Available since

   Feb. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. TERT - WT

    Plasmid

    #213827

    Purpose

    Expresses human telomerase reverse transcriptase, the catalytic subunit of telomerase

    Depositor

    Coleman Lindsley

    Article

    Reilly et al Blood. 2021 Sep 9;138(10):898-911. doi:

    Insert

    human TERT (TERT Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tet-responsive promoter PTight, consisting of sev…

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Ai133 (TITL-ssAPEX2tm) targeting vector

    Plasmid

    #114432

    Purpose

    Target a Cre-dependent electron microscopy tag ssAPEX2tm cassette into the mouse TIGRE locus

    Depositor

    Hongkui Zeng

    Article

    Daigle et al Cell. 2018 Jul 12;174(2):465-480.e22. do

    Insert

    ssAPEX2tm
    

    Use

    Cre/Lox and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    TREtight

    Available since

    Jan. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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