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pDEST47-ARL14

Plasmid

#67469

Purpose

Mammalian expression of hArl14 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL14 (ARL14 Human)

Expression

Mammalian

Mutation

bp 172 C to T; aa L58F

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL11

Plasmid

#67470

Purpose

Mammalian expression of hArl11 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL11 (ARL11 Human)

Expression

Mammalian

Mutation

bp 261 G to A; silent mutation; bp 442 T to C; aa…

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL5A

Plasmid

#67324

Purpose

hArl5a with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5A (ARL5A Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 70 C to T; silent mutation

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL5B

Plasmid

#67326

Purpose

hArl5b with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL5B (ARL5B Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL6

Plasmid

#67328

Purpose

hArl6 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL4D

Plasmid

#67336

Purpose

hArl4D with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4D (ARL4D Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 272 A to C; N91T mutation; bp 327 T to G; sil…

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL14

Plasmid

#67338

Purpose

hArl14 with stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL14 (ARL14 Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 172 C to T; aa L58F

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
ITPKA-mScarlet-I

Plasmid

#98829

Purpose

In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

ITPKA

Tags

mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2017

Availability

Academic Institutions and Nonprofits only

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PH-PLCD1_mScarlet_IRES_SYFP2_PH_N1

Plasmid

#85069

Purpose

Yellow/red FRET biosensor for monotoring plasmamembrane PtdIns(4,5)P2 levels (PIP2)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

PH-PLCD1 (PLCD1 Human)

Tags

IRES-sYFP2-PH and mScarlet

Expression

Mammalian

Mutation

PLCD aa 1–170

Promoter

CMV

Available Since

Oct. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/CD63mScarlet

Plasmid

#182972

Purpose

Stably express CD63 fused with mScarlet in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

CD63-mScarlet (CD63 Human)

Expression

Mammalian

Mutation

N/A

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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ymScarlet

Plasmid

#111917

Purpose

yeast optimized mScarlet

Depositor

Patrick Lajoie

Article

Albakri et al F1000Res. 2018 Aug 10;7:1242. doi: 10.12

Insert

ymScarlet

Expression

Yeast

Promoter

GPD

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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3xmScarletI_UtrCH

Plasmid

#112962

Purpose

In vivo visualization of F-actin (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

Insert

UtrCH

Tags

3x mScarlet-I

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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VPS13D^mScarlet

Plasmid

#174110

Purpose

The insert is a codon optimized DNA sequence encoding VPS13D with an internal mScarlet protein.

Depositor

Pietro De Camilli

Article

Guillen-Samander et al J Cell Biol. 2021 May 3;220(5). pii: 212

Insert

Codon optimized VPS13D

Tags

mScarlet

Expression

Mammalian

Mutation

With an internal mScarlet tag in aminoacid positi…

Promoter

CMV

Available Since

Dec. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pmScarlet-HSP70

Plasmid

#163790

Purpose

Expresses mScarlet-Hsp70 in mammalian cells

Depositor

Vincent Timmerman

Article

Adriaenssens et al Sci Rep. 2020 May 29;10(1):8755. doi: 10

Insert

HSP70 (HSPA1A Human)

Tags

mScarlet

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pET-DEST42-ARL6-V5-His6

Plasmid

#67422

Purpose

Expression of hArl6 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL4-V5-His6

Plasmid

#67419

Purpose

Expression of hArl4 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Tags

His and V5

Expression

Bacterial

Mutation

bp 105 A to T; silent mutation

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL4C-V5-His6

Plasmid

#67423

Purpose

Expression of hArl4c with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Tags

His and V5

Expression

Bacterial

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-ARL6-V5-His6

Plasmid

#67443

Purpose

Mammalian expression of hArl6 with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL6 (ARL6 Human)

Tags

His and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST40-ARL8B-V5-His6

Plasmid

#67446

Purpose

Mammalian expression of hArl8b with C-term V5 and His6 tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL8B (ARL8B Human)

Tags

His and V5

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDSHA-ARL4-Myc

Plasmid

#67356

Purpose

Mammalian expression of hArl4 with C-term Myc tag

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only