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Showing: 381 - 400 of 8125 results

  5. pBridge-tyrosinase.σ2

    Plasmid
    #197415
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-2 σ2 fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-2 σ2
    Use
    Tags
    GAL4-DNA binding domain fragment, HA tag, and nuc…
    Expression
    Yeast
    Mutation
    encodes R42G substitution, contains silent substi…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197415-plasmid-map-sequence-id-388893

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  16. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site1)

    Plasmid
    #62580
    Purpose
    Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The upstream miR-21 (site 1) binding site was mutated.
    Depositor
    Michael Ruppert
    Article
    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6
    Insert
    SPRED1 (SPRED1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    miR-21 binding site 1 mutant (TAAGCTA --> TAGA…
    Promoter
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  17. pMIR-Report-SPRED1-3'UTR (mut - miR-21 site 2)

    Plasmid
    #62581
    Purpose
    Translational Luciferase Reporter encoding a mutated fragment of the SPRED1 3'UTR. The downstream miR-21 binding site (site 2) was mutated.
    Depositor
    Michael Ruppert
    Article
    Sharma et al Mol Cell Biol. 2014 Nov 15;34(22):4143-6
    Insert
    SPRED1 (SPRED1 Human)
    Use
    Luciferase
    Tags
    Expression
    Mutation
    miR-21 binding site 2 mutant (TAAGCTA --> TAGA…
    Promoter
    Available since
    June 4, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pGem-sHyper:sAPX

    Plasmid
    #84738
    Purpose
    Simultaneous expresion of stromal sHyPer and stromal Ascorbate peroxidase (APX) in plant cells
    Depositor
    Philip Mullineaux
    Article
    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10
    Inserts
    sAPX (SAPX Mustard Weed)
    sHyper (HyPer2 targeted to nucleus)
    Use
    Tags
    nuclear localisation signals from the coding sequ…
    Expression
    Plant
    Mutation
    Promoter
    pFMV/p35S
    Available since
    July 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    84738-plasmid-map-sequence-id-180317

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  19. pGem-nHyPer:sAPX

    Plasmid
    #84735
    Purpose
    Simultaneous expresion of stromal HyPer2 and stromal Ascorbate peroxidase (APX) in plant cells
    Depositor
    Philip Mullineaux
    Article
    Exposito-Rodriguez et al Nat Commun. 2017 Jun 29;8(1):49. doi: 10
    Inserts
    sAPX (SAPX Mustard Weed)
    nHyper (HyPer2 targeted to nucleus)
    Use
    Tags
    nuclear localisation signals from the coding sequ…
    Expression
    Plant
    Mutation
    Promoter
    FMV/35S
    Available since
    July 6, 2017
    Availability
    Academic Institutions and Nonprofits only
    84735-plasmid-map-sequence-id-180316

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  20. pBridge-tyrosinase.σ1A

    Plasmid
    #197381
    Purpose
    Expression of 1) GAL4 DNA-binding domain (BD)-tyrosinase cytosolic tail fusion protein, 2) HA epitope and nuclear localization signal (NLS) AP-1 σ1A fusion protein in yeast (yeast three-hybrid assays)
    Depositor
    Juan Bonifacino
    Article
    Mattera et al J Biol Chem. 2011 Jan 21;286(3):2022-30.
    Inserts
    tyrosinase cytosolic tail
    AP-1 σ1A
    Use
    Tags
    GAL4-DNA binding domain fragment, HA tag, and SV4…
    Expression
    Yeast
    Mutation
    Contains an extra 42 nucleotides encoding 14 resi…
    Promoter
    ADH1 and MET25
    Available since
    March 28, 2023
    Availability
    Academic Institutions and Nonprofits only
    197381-plasmid-map-sequence-id-388878

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Showing: 381 - 400 of 8125 results