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Plasmid#172174PurposePylB overexpression and purificationDepositorInsertPT7/Lac-[His6-TEV-SUMO-PylB.JM10.1]
UseTagsExpressionBacterialMutationpET28a_LacI_PT7/Lac-[His6-TEV-SUMO-PylB(N61S, E84…PromoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDM_N1_400
Plasmid#170474PurposeTo amplify transcription template containing LacI operatorsDepositorInsertT7A1, O-, O1, Lambda t1 in modified lacZ fragment
UseTagsExpressionBacterialMutationdiminished hairpins near O1 and diminished latent…PromoterT7A1Available sinceSept. 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgiRFP1
Plasmid#113972PurposeSingle short guide RNA targeting GATCGAGTTCGAGCCTGCGG in iRFP670 sequenceDepositorInsertiRFP670 sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgiRFP2
Plasmid#113973PurposeSingle short guide RNA targeting GCGCGTTCTTTGGACGCGA in iRFP670 sequenceDepositorInsertiRFP670 sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
sgiRFP3
Plasmid#113974PurposeSingle short guide RNA targeting CGTGATGTTGTACCGCTTC in iRFP670 sequenceDepositorInsertiRFP670 sgRNA
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
sg1
Plasmid#113966PurposeSingle short guide RNA targeting GTATAGCATACATTATACGDepositorInsertsg1
UseTagsExpressionMutationPromoterU6Available sinceMay 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 uvrA
Plasmid#112892PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertuvrA-like protein
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 stat
Plasmid#112886PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertstat
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAWK
Plasmid#62713PurposeDonor vector for amiR-eGFP knock-in via landing padDepositorInsertamiR-eGFP123/amiR-eGFP419/FLPe
UseRNAiTagsExpressionMammalianMutationPromoterAvailable sinceDec. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSL5 SMR1713
Plasmid#28028DepositorInsertMutL (mutL E. coli)
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 1, 2011AvailabilityAcademic Institutions and Nonprofits only -
pEND-349_Cacnes_ori
Plasmid#225604PurposeCutibacterium acnes replicative plasmid for recombinant expression. pBRESP36A-based vector with C. acnes chloramphenicol resistance, harbouring a medium-copy pBR322 E. coli ori (no ROP)DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
pET30b_OmpAT7Sav
Plasmid#138589PurposeT7-tagged streptavidin with native C-terminus and periplasmic export signal OmpA; controlled by PT7 promoter; KanR, pBR322 oriDepositorInsertT7-tagged streptavidin variant with native C-terminus and OmpA signal peptide for periplasmic export
UseSynthetic BiologyTagsOmpA export signal and T7 tagExpressionBacterialMutationPromoterPT7Available sinceMarch 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSc-humanADAR2
Plasmid#171106PurposeExpresses human ADAR2 in yeast.DepositorInserthuman ADAR2 (ADARB1 Human)
UseTagsHis12_TEVExpressionYeastMutationPromoterGAL1Available sinceAug. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
CLYBL_Bxb1-GA_LP_Dual_TC
Plasmid#229776PurposeCLYBL targeting construct with the Bxb1-GA landing pad cassette.DepositorInsertFRT-mScarlet-attP(GA)-PuroR-F3
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterpGKAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_CAG_3xTurboGFP-NLS
Plasmid#229777PurposeBxb1-GT donor plasmid for integrating a transcription unit for constitutive expression of nuclear-localised TurboGFP (3 copies)DepositorInsert3x TurboGFP
UseSynthetic BiologyTagsNLSExpressionMammalianMutationPromoterCAGAvailable sinceMarch 3, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GT_AIO-TetOn_Divergent Donor
Plasmid#229784PurposeBxb1-GT donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)DepositorInsertLacZ; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE and CAGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_AIO-TetOn_Divergent Donor
Plasmid#229786PurposeBxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible expression (empty)DepositorInsertLacZ; rtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterTRE and CAGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pegRNA2_tevopreQ1
Plasmid#212039PurposeBacterial expression of the pegRNA for genome editing in E. coliDepositorInsertpegRNA
UseCRISPRTagsExpressionMutationPromoterJ23119Available sinceApril 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AY27_pU6.gRNA.CLYBL
Plasmid#199238PurposeExpression construct encoding a S. pyogenes guide RNA targeting the human CLYBL safe harbor locusDepositorInsertS. pyogenes gRNA spacer
UseCRISPR and Synthetic BiologyTagsExpressionMammalianMutationPromoterRNA polymerase III promoter for human U6 snRNAAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only -
AD59_pEP.DonorCLYBL.TS
Plasmid#199227PurposeDonor plasmid with CLYBL target sites for ITPN or HMEJ knock-in at the human CLYBL safe harbor locusDepositorInsertExpression unit for PuroR.T2A.EGFP selectable and reporter markers
UseGene targeting donor plasmidTagsExpressionMammalianMutationPromoterHuman PGK1 gene promoterAvailable sinceApril 28, 2023AvailabilityAcademic Institutions and Nonprofits only