We narrowed to 2,259 results for: poli

1. pPTG-GG-sg2+sg3

   Plasmid

   #127199

   Purpose

   Plasmid expressing sgRNA2 (TACCACATTTGTAGAGGTT) & sgRNA3 (CAATGTATCTTATCATGTC) as polycistronic tRNA-gRNA from single U6 promoter

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   tRNA-gRNA
   

   Use

   Empty sgrna plasmid

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pN3-sTRAIL

   Plasmid

   #154246

   Purpose

   Plasmid compatible with non-viral delivery for secretion of trimeric human TRAIL

   Depositor

   Jordan Green

   Article

   Vaughan et al Mol Ther Oncolytics. 2021 Apr 16;21:377-

   Insert

   Secretable Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV Promoter

   Available since

   July 29, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ubc-c005

   Plasmid

   #216876

   Purpose

   Bacterial expression vector for E. coli producing ubiquitin with N-terminal YFP fusion

   Depositor

   Lari Lehtiö

   Article

   Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

   Insert

   Ubiquitin (UBC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. Ubc-c004

   Plasmid

   #216875

   Purpose

   Bacterial expression vector for E. coli producing ubiquitin with N-terminal CFP fusion

   Depositor

   Lari Lehtiö

   Article

   Vela-Rodriguez et al SLAS Discov. 2023 Dec;28(8):365-375. doi

   Insert

   Ubiquitin (UBC Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX6P-1-eIF4E K119A

   Plasmid

   #199440

   Purpose

   Bacterial expression plasmids for production of recombinant GST-eIF4E K119A

   Depositor

   Shin-ichi Hoshino

   Article

   Ogami et al STAR Protoc. 2023 May 26;4(2):102340. do

   Insert

   1977 (EIF4E Human)
   

   Use

   Tags

   GST-fusion protein

   Expression

   Bacterial

   Mutation

   changed lysine 119 to alanine

   Promoter

   Available since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACEBac1-PARP1-Flag-His-WT

   Plasmid

   #111572

   Purpose

   Expresses full length PARP1 in Sf9 (insect) cells

   Depositor

   Thomas Muir

   Article

   Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

   Insert

   PARP1 (PARP1 Human)
   

   Use

   Tags

   C-terminal 6xHis and C-terminal Flag

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin promoter

   Available since

   July 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA-Stra6L-6His

   Plasmid

   #174215

   Purpose

   Stra6L-6His (N-Terminal) fusion protein driven by an CMV promotor. May be used to study the murine Vitamin A receptor Stra6L.

   Depositor

   Adrian Press

   Article

   Stafast et al ACS Appl. Polym. Mater. 2022

   Insert

   Stra6L (Stra6l Mouse)
   

   Use

   Tags

   6-His

   Expression

   Mammalian

   Mutation

   non

   Promoter

   CMV

   Available since

   Aug. 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-EF1A-LivePAR(R163A)-Hygro

   Plasmid

   #187609

   Purpose

   EGFP fused to the C-terminus of a WWE domain containing the mutation Arg163Ala & a hygromycin resistance cassette.

   Depositor

   Robert Sobol

   Article

   Koczor et al Cancers (Basel). 2022 Jul 28;14(15). pii

   Insert

   LivePAR(R163A)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   R163A mutation in the WWE Domain

   Promoter

   EF1A

   Available since

   Sept. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. sg1+sg2+sg3

   Plasmid

   #113969

   Purpose

   Triple short guide RNA targeting GTATAGCATACATTATACG, TACCACATTTGTAGAGGTT & CAATGTATCTTATCATGTC

   Depositor

   Jordan Green

   Article

   Rui et al Mol Ther Nucleic Acids. 2020 Apr 21;20:6

   Insert

   sg1+sg2+sg3
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. C211

    Plasmid

    #174360

    Purpose

    For expressing siRNA-resistant human PABPC1 mutant (I110L, D111E, D117E, A121G, G139A, Y140F, T147S, R166K) in human cell lines via transfection

    Depositor

    David Bartel

    Article

    Xiang et al Elife. 2021 Jul 2;10. pii: 66493. doi: 1

    Insert

    PABPC1 (PABPC1 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    I110L, D111E, D117E, A121G, G139A, Y140F, T147S, …

    Promoter

    Available since

    Feb. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.15 Sense

    Plasmid

    #46839

    Purpose

    Express cGFP transcript ending in a mutant MALAT1 triple helix that causes the transcript to be stable but poorly translated

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCRII-TOPO CMV-cGFP-mMALAT1_3' Comp.14 Sense

    Plasmid

    #46838

    Purpose

    Express cGFP transcript ending in the minimal functional MALAT1 triple helix

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCRII-TOPO CMV-cGFP-mMALAT1_3' Mut U1/U2 Sense

    Plasmid

    #46837

    Purpose

    Express cGFP transcript ending in a mutant MALAT1 triple helix which causes the transcript to be degraded

    Depositor

    Phil Sharp

    Article

    Wilusz et al Genes Dev. 2012 Nov 1;26(21):2392-407. d

    Insert

    Coral Green Fluorescent Protein (cGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 25, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. polyQ74-GFP

    Plasmid

    #231893

    Purpose

    Forms cytosolic HTT partial exon 1 Q74 aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74 (HTT Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Promoter

    Available since

    March 19, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. GFP-polyQ74-NLS

    Plasmid

    #231892

    Purpose

    Forms nuclear HTT partial exon 1 Q74-NLS aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74-NLS (HTT Human)
    

    Use

    Tags

    EGFP and SV40 NLS

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Promoter

    Available since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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16. GFP-polyQ23-NLS

    Plasmid

    #231891

    Purpose

    Expression of non-aggregated HTT partial exon 1 Q23-NLS in the nucleus

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q23-NLS (HTT Human)
    

    Use

    Tags

    EGFP and SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-PARP1-3xFlag-7xKQ

    Plasmid

    #111576

    Purpose

    Expresses full length PARP1 with K to Q mutations in automodification domain in mammalian cells

    Depositor

    Thomas Muir

    Article

    Liszczak et al Nat Chem Biol. 2018 Jul 16. pii: 10.1038

    Insert

    PARP1 (PARP1 Human)
    

    Use

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    lysine to glutamine mutation at the following ami…

    Promoter

    CMV

    Available since

    July 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCI-MS2V5-eRF3a F76a

    Plasmid

    #65811

    Purpose

    expression of eRF3a-MS2 fusion. F76A mutation abolishes binding to PABPC1

    Depositor

    Niels Gehring

    Article

    Fatscher et al RNA. 2014 Oct;20(10):1579-92. doi: 10.12

    Insert

    eRF3a (GSPT1 Human)
    

    Use

    Tags

    MS2 stem loops and V5

    Expression

    Mammalian

    Mutation

    F76a

    Promoter

    CMV

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAG32-3HA-3FLAG

    Plasmid

    #154081

    Purpose

    For C-terminal 3xHA-3xFLAG-protein tagging in S. pombe using hphMX4 as resistance marker.

    Depositor

    Cornelia Kilchert

    Article

    Kilchert et al Genome Res. 2020 Jul;30(7):1012-1026. do

    Insert

    3xHA-3xFLAG-hygR
    

    Use

    Pcr-based tagging in yeast

    Tags

    3xHA-3xFLAG

    Expression

    Mutation

    Promoter

    Available since

    Sept. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. PABPC1_544-626_pGEX6P1

    Plasmid

    #209790

    Purpose

    Bacterial expression of PABPC1 Mlle domain

    Depositor

    Kalle Gehring

    Article

    Kozlov et al J Biol Chem. 2010 Apr 30;285(18):13599-6

    Insert

    Mlle domain of PABPC1 (PABPC1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    aa 544-626 only

    Promoter

    tac

    Available since

    Nov. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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