We narrowed to 9,363 results for: sacs

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pX600-AAV-CMV::NLS-SaCas9-NLS-3xHA-bGHpA

Plasmid

#61592

Purpose

A human codon-optimized Cas9 from Staphylococcus aureus (SaCas9).

Depositor

Feng Zhang

Article

Ran et al Nature. 2015 Apr 1. doi: 10.1038/nature1

Has Service

AAV6 and AAV8

Insert

hSaCas9

Use

AAV and CRISPR

Tags

3xHA and NLS

Expression

Mammalian

Available Since

Feb. 17, 2015

Availability

Academic Institutions and Nonprofits only

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1313_pAAV-U6-SA-BbsI-MluI-gRNA-HLP-SACas9-HA-OLLAS-spA

Plasmid

#109314

Purpose

Plasmid for liver-specific expression of AAV SaCas9 with a BbsI cloning site for a gRNA

Depositor

William Lagor

Article

Li et al Mol Ther Methods Clin Dev. 2018 Dec 6;12

Type

Empty backbone

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-SA-WTmLdlrEx14-gRNA2-N22-HLP-SACas9-HA-OLLAS-spA

Plasmid

#206860

Purpose

An AAV plasmid with U6 promoter driving a gRNA against LDLR and liver specific HLP promoter driving SaCas9

Depositor

William Lagor

Article

De Giorgi et al Methods Mol Biol. 2022;2419:673-713. doi

Insert

mLdlr gRNA (Ldlr Mouse)

Use

AAV and CRISPR

Available Since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pLV hU6-gRNA hUbC-dSaCas9-KRAB-T2A-Thy1.1

Plasmid

#194278

Purpose

Expresses gRNA and dSaCas9-KRAB from lentiviral vector

Depositor

Charles Gersbach

Article

McCutcheon et al bioRxiv. 2023 May 1:2023.05.01.538906. d

Inserts

humanized dSaCas9 KRAB T2A Thy1.1
gRNA

Use

CRISPR and Lentiviral

Tags

HA

Expression

Mammalian

Promoter

hU6 and hUbC

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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Coxsackievirus A16 (CVA16) isolate ENT-2012-0948 3C protease

Plasmid

#228637

Purpose

Bacterial expression of the Coxsackievirus 16 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

Coxsackievirus 16 3C protease

Tags

His-SUMO

Expression

Bacterial

Available Since

Feb. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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KZ801: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; EF1a promoter

Plasmid

#121814

Purpose

pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-002 Esp3I//(A)-BsaI-SacB-BsaI-(Z)//Esp3I

Plasmid

#130177

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

SacB

Use

Synthetic Biology

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-007 Esp3I//(A)-AarI-SacB-AarI-(Z)//Esp3I

Plasmid

#130215

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

SacB

Use

Synthetic Biology

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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KB501: pMAGIC (L1-R5) hU6::SaCas9 gRNA scaffold; CMV promoter

Plasmid

#121813

Purpose

pMAGIC L1-R5 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion.

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Available Since

March 22, 2019

Availability

Academic Institutions and Nonprofits only

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KB601: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; EF1a promoter

Plasmid

#121819

Purpose

pMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and EF1a promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Synthetic Biology; Pmagic gateway entry plasmid

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-001 BsaI//(A)-Esp3I-SacB-Esp3I-(Z)//BsaI

Plasmid

#130176

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

SacB

Use

Synthetic Biology

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-004 Esp3I//(A)-AarI-SacB-AarI-(Z)//Esp3I

Plasmid

#130179

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

SacB

Use

Synthetic Biology

Available Since

Feb. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-008 Esp3I//(A)-BsaI-SacB-BsaI-(Z)//Esp3I

Plasmid

#130216

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

SacB

Use

Synthetic Biology

Available Since

Feb. 9, 2020

Availability

Academic Institutions and Nonprofits only

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Coxsackievirus B4 (CVB4) 3C protease isolate CVB4/USA/9R1/2012

Plasmid

#228640

Purpose

Bacterial expression of the Coxsackievirus 43 isoform B 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

Coxsackievirus 43 isoform B 3C protease

Tags

His-SUMO

Expression

Bacterial

Available Since

Feb. 18, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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Coxsackievirus A6 (CVA6) strain JLS10/HeN/CHN/2019 3C protease

Plasmid

#228638

Purpose

Bacterial expression of the Coxsackievirus 63 3C protease

Depositor

ASAP Discovery, Frank von Delft

Insert

Coxsackievirus 63 3C protease

Tags

His-SUMO

Expression

Bacterial

Available Since

Feb. 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pBK1876-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA2)

Plasmid

#223162

Purpose

Expression of KRAB and truncated MECP2 with dSaCas9 and gRNA2 targeting human/mouse APOE

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 23, 2024

Availability

Academic Institutions and Nonprofits only

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pBK1861-AAV-EFSNC-dSaCas9-KRAB-MECP2(APOE-gRNA1)

Plasmid

#223161

Purpose

Expression of KRAB and truncated MECP2 with dSaCas9 and gRNA1 targeting human/mouse APOE

Depositor

Boris Kantor

Article

Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

Insert

KRAB-MECP2 (MECP2 Human)

Use

AAV and CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

Encodes aa 204-310 (MECP2)

Promoter

EF1a

Available Since

Aug. 22, 2024

Availability

Academic Institutions and Nonprofits only

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pCA16-Ubc-NLS-HA-dSaCas9-NLS-[10XGCN4]-P2A-BSD

Plasmid

#199447

Purpose

Expession of dSaCas9-10XGCN4 that recruits scFV-tdTomato (pCA15) for amplifying fluorescent signal

Depositor

Baohui Chen

Article

Xu et al J Virol. 2022 Dec 21;96(24):e0092022. do

Insert

dSaCas9-10xGCN4

Use

Lentiviral

Expression

Mammalian

Promoter

human ubiquitin C (UBC)

Available Since

May 4, 2023

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-011 BsaI//(U)-Esp3I-SacB-Esp3I-(V)//BsaI

Plasmid

#130242

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

SacB

Use

Synthetic Biology

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

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pRDAX-EMMI-015 AarI//(U)-BsaI-SacB-BsaI-(V)//AarI

Plasmid

#130246

Purpose

EMMA Kit # 1000000119 extension

Depositor

Gary Lewin

Insert

SacB

Use

Synthetic Biology

Available Since

Sept. 9, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge