We narrowed to 986 results for: transfection

1. pLAP-MIS12-KARD

   Plasmid

   #114057

   Purpose

   transfection in mammalian cells, combination of fluorescent protein fusion and tandem affinity purification

   Depositor

   Geert Kops

   Article

   Suijkerbuijk et al Dev Cell. 2012 Oct 16;23(4):745-55. doi:

   Insert

   MIS12-KARD (BUB1B Human)
   

   Tags

   YFP-TEV-S- Human LAP

   Expression

   Mammalian

   Available Since

   Sept. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. FHpHG_mTent5c_dCAT

   Plasmid

   #221442

   Purpose

   Transfection into the cells for the reporter or qPCR assay.

   Depositor

   Marcos Morgan

   Article

   Li et al FEBS J. 2025 May 27. doi: 10.1111/febs.7

   Insert

   Tent5c_AA (dCAT) (Fam46c Mouse)
   

   Tags

   FLAG-2xHA-(HRV 3C site)-6xHis-EGFP

   Expression

   Mammalian

   Mutation

   D90A, D92A (GATctgGAT to GCCctgGCC)

   Available Since

   June 12, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pGL4.23-Gja1prom

   Plasmid

   #188114

   Purpose

   Expresses luciferase under control of Gja1 promoter in transfected cells

   Depositor

   Jennifer Mitchell

   Article

   Shchuka et al PLoS One. 2023 Jan 3;18(1):e0271081. doi

   Insert

   Gja1 promoter (Gja1 Mouse)
   

   Use

   Luciferase

   Promoter

   Gja1 endogenous promoter up to gene start codon

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTOPO-HqERV(del5'-LTR)

   Plasmid

   #164089

   Purpose

   transfection of Hq-ERV without 5'-LTR

   Depositor

   Maik Hintze

   Article

   Hintze et al Mamm Genome. 2020 Dec 26. pii: 10.1007/s

   Insert

   Hq-ERV
   

   Use

   Eukaryotic expression vector

   Mutation

   deletion of 5'-LTR

   Promoter

   none (5'-LTR deleted)

   Available Since

   Jan. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAS_4xhyb PylT A41AA C55A LaG17-SynNotch 204TAA 442TAG

   Plasmid

   #154778

   Purpose

   plasmid with 4xhybrid PylT cassette (mutant A41AA C55A) and LaG17-SynNotch 204TAA 277TAG, for transient transfection or stable, piggybac-mediated, integration

   Depositor

   Simon Elsaesser

   Article

   Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

   Insert

   LaG17-SynNotch
   

   Tags

   LaG17

   Expression

   Mammalian

   Mutation

   204TAA 442TAG in LaG17-SynNotch, hybrid PylT with…

   Promoter

   EF1

   Available Since

   Oct. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. CDK1as_T2A_puromycin

   Plasmid

   #118595

   Purpose

   One of three plasmids required to create CDK1as human cells by single transfection (One shot system). CDK1as cDNA and puromycin resistance cassette flanked by Sleeping Beauty transposon.

   Depositor

   William Earnshaw

   Article

   Saldivar et al Science. 2018 Aug 24;361(6404):806-810.

   Insert

   CDK1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. CMV-Nme2ABE8e

   Plasmid

   #189928

   Purpose

   Plasmid encoding Nme2ABE8e driven by the CMV promoter for plasmid transfection.

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   Nme2ABE8e
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Nme2Cas9 D16A

   Promoter

   CMV

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. CMV-CjABE8e

   Plasmid

   #189929

   Purpose

   Plasmid encoding CjABE8e driven by the CMV promoter for plasmid transfection.

   Depositor

   David Liu

   Article

   Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

   Insert

   CjABE8e
   

   Expression

   Mammalian

   Mutation

   CjCas9 D8A

   Promoter

   CMV

   Available Since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1+_hsTRPA1_myc_dTom

   Plasmid

   #183179

   Purpose

   myc tagged hsTRPA1 used for cell transfection

   Depositor

   Sreekanth Chalasani

   Article

   Duque et al Nat Commun. 2022 Feb 9;13(1):600. doi: 1

   Inserts

   hsTRPA1 (TRPA1 Human)
   dTom
   

   Expression

   Mammalian

   Available Since

   May 26, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. 7M8

    Plasmid

    #64839

    Purpose

    This plasmid can be used in the triple transfection method of AAV vector production. It supplies the replication proteins from AAV2 and the 7M8 capsid protein.

    Depositor

    John Flannery, David Schaffer

    Article

    Dalkara et al Sci Transl Med. 2013 Jun 12;5(189):189ra

    Insert

    7M8 cap
    

    Use

    AAV

    Mutation

    7mer insertion in the AAV2 cap

    Available Since

    Aug. 3, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pHcRed-NPM1c-C1

    Plasmid

    #131821

    Purpose

    Expresses HcRed-NPM1c (cytoplasmic mutant, human) in mammalian cells through transient transfection

    Depositor

    Mark Minden

    Article

    Wang et al Leukemia. 2020 May;34(5):1278-1290. doi:

    Insert

    NPM1 (NPM1 Human)
    

    Tags

    HcRed

    Expression

    Mammalian

    Mutation

    This is the cytoplasmic mutant causing AML, where…

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. PB-CAG-dCasRx-RBM25-IRES-BleoR

    Plasmid

    #221002

    Purpose

    Stable expression of dCasRx-RBM25 when co-transfected with piggyBac transposase. Zeocin selection. CAG promoter. Use with lentiviral gRNA delivery (Addgene #138150) to stably modulate splicing.

    Depositor

    Benjamin Blencowe, Mikko Taipale

    Article

    Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

    Insert

    dCasRx-RBM25-IRES-BleoR
    

    Use

    CRISPR

    Tags

    NLS and NLS-HA

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    June 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. qTAG-N-Solo-mStayGold-TUBA1B

    Plasmid

    #227327

    Purpose

    Donor template for mStayGold insertion into the N-terminus of the TUBA1B locus. For tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B (Addgene #207763)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a mStayGold Tag (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. Activin A (S2-2)

    Plasmid

    #184621

    Purpose

    for transfection into S2 cells and expression of Activin A protein

    Depositor

    Timothy Springer

    Article

    Le et al J Mol Biol. 2022 Mar 15;434(5):167439. d

    Insert

    INHBA (INHBA Human)
    

    Tags

    8x His - streptavidin binding peptide

    Expression

    Insect

    Available Since

    June 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. qTAG-N-Solo-mScarlet-TUBA1B

    Plasmid

    #207764

    Purpose

    Donor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    TUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 15, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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16. pCAG-eCas9-T2A-EGFP-U6-gRNA-no ITR and f1 ori

    Plasmid

    #234724

    Purpose

    All-in-one CRISPR/Cas9 vector with high-fidelity eSpCas9 expression in neurons. The plasmid lacks AAV2 ITR and f1 ori elements, enabling more efficient transfection and expression.

    Depositor

    Kirill Martemyanov

    Article

    Zhao et al Nat Commun. 2025 Apr 22;16(1):3768. doi:

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    T2A-GFP

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    April 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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17. qTAG-C-mScarlet-Puro-H2BC11

    Plasmid

    #207761

    Purpose

    Donor template for mScarlet-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a mScarlet-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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18. CMV-SauriABE8e

    Plasmid

    #189927

    Purpose

    Plasmid encoding SauriABE8e driven by the CMV promoter for plasmid transfection.

    Depositor

    David Liu

    Article

    Davis et al Nat Biomed Eng. 2022 Jul 28. pii: 10.103

    Insert

    SauriABE8e
    

    Expression

    Mammalian

    Mutation

    Nme2Cas9 D16A

    Promoter

    CMV

    Available Since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. qTAG-C-moxGFP-Blast-H2BC11

    Plasmid

    #207757

    Purpose

    Donor template for moxGFP-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a moxGFP-Blast Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. IL1RN gRNA3

    Plasmid

    #113132

    Purpose

    Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

    Depositor

    Dominik Niopek

    Article

    Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

    Insert

    IL1RN guideRNA 3 (SpCas9 scaffold), co-expressed GFP (transfection marker)
    

    Use

    AAV, CRISPR, and Synthetic Biology

    Expression

    Mammalian

    Available Since

    Nov. 1, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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