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ZP44 Wnt8 5' orf2

Plasmid

#16898

Depositor

Randall Moon

Insert

wnt8 5'orf2 (wnt8a Zebrafish)

Expression

Bacterial

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
ZE11 tiggywinkle hedgehog CMV

Plasmid

#17060

Depositor

Randall Moon

Insert

tiggywinkle hedgehog (shhb Zebrafish)

Available since

Feb. 1, 2008

Availability

Academic Institutions and Nonprofits only
MiniCoopR 2xU6:gRNA pten, mitfa:Cas9

Plasmid

#118845

Purpose

Targets ptena and ptenb and expresses zebrafish mitfa specifically in zebrafish melanocytes

Depositor

Leonard Zon

Publication

Ablain et al Science. 2018 Nov 1. pii: science.aau650

Insert

ptena and ptenb (ptena, ptenb Zebrafish)

Use

CRISPR; Tol2

Available since

March 21, 2019

Availability

Academic Institutions and Nonprofits only

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pRL093 pattB_exorh:EGFP_MCS

Plasmid

#213514

Purpose

attB-containing vector backbone, for use in restriction enzyme- or PCR-based cloning

Depositor

Christian Mosimann

Publication

Lalonde et al Sci Adv. 2024 Jun 7;10(23):eadn6603. doi

Type

Empty backbone

Use

Zebrafish expression

Available since

Feb. 23, 2024

Availability

Academic Institutions and Nonprofits only

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PhOTO-C (pMTB:cyto-Dendra2-2A-H2B-Cerulean)

Plasmid

#92402

Purpose

Constitutive expression of cytosolic green-to-red photoconvertible Dendra2 and the nuclear blue fluorescent protein Cerulean.

Depositor

Periklis Pantazis

Publication

Kalyviotis et al (2020) Behavioral and Neural Genetics of

Inserts

Dendra2
Cerulean

Use

Zebrafish expression

Tags

H2B tag and No tag, cytosolic expression

Promoter

bactin2

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; gcry1:BFP -2

Plasmid

#173888

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Publication

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; gcry1:BFP -0

Plasmid

#173886

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Publication

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; gcry1: BFP

Use

Synthetic Biology

Available since

Aug. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pCrysβ:ECFP-LexOP:mCherry-KrasG12V

Plasmid

#89888

Purpose

Human KRasG12V fused to mCherry downstream of LexOP, placed within a non-autonomous maize dissociation (Ds) element for integration in zebrafish. Contains pCrysB-ECFP for screening.

Depositor

Tatjana Sauka-Spengler

Publication

Kenyon et al Dis Model Mech. 2018 Apr 6;11(4). pii: d

Insert

mCherry-KRasG12V

Use

Unspecified

Tags

mCherry

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST mfap4:tdTomato

Plasmid

#135214

Purpose

Red tagged macrophages

Depositor

David Tobin

Publication

Walton et al PLoS One. 2015 Oct 7;10(10):e0138949. do

Insert

mfap4 promoter and tdTomato

Use

Zebrafish expression

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Tol2_B-act_dCas9-Vp64-T2A-Citrine

Plasmid

#200248

Purpose

Zebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesis

Depositor

Tatjana Sauka-Spengler

Publication

Pelea et al bioRxiv 2023.05.08.539738

Insert

dCas9-Vp64-T2A-Citrine

Use

Zebrafish expression

Available since

July 18, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2+ gfp-zASC

Plasmid

#98703

Purpose

GFP tagged zebrafish ASC in the pCS2+ vector for in vitro transcription

Depositor

Anna Huttenlocher

Publication

Vincent et al Cell Microbiol. 2016 Apr;18(4):591-604.

Insert

Apoptosis associated speck like protein containing CARD (pycard Zebrafish)

Use

Zebrafish/xenopus expression

Tags

GFP

Expression

Mammalian

Available since

Sept. 27, 2017

Availability

Academic Institutions and Nonprofits only

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pcs2+spCas9 ABE-Umax-nanos

Plasmid

#222142

Purpose

Adenine base editor with modified TadA variant for germline A-to-G base editing in zebrafish

Depositor

Gaurav Varshney

Publication

Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

Insert

Modified TadA-nSpCas9 with nanos UTR

Use

CRISPR; Zebrafish expression

Tags

Bipartite NLS

Promoter

SP6

Available since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pUFlip-floxed2A-mRFP-; HE1A:BFP -0

Plasmid

#180007

Purpose

Donor for generating conditional alleles in zebrafish using precise CRISPR directed genomic integration with the GeneWeld method

Depositor

Jeffrey Essner

Publication

Liu et al Elife. 2022 Jun 17;11. pii: 71478. doi:

Insert

2A-mRFP; HE1A: BFP

Use

Synthetic Biology

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-gfap-sgRNA

Plasmid

#65566

Purpose

in vitro trancription of sgRNA targeting the zebrafish gfap locus

Depositor

Jiu-lin Du

Publication

Li et al Cell Res. 2015 Apr 7. doi: 10.1038/cr.20

Insert

gfap sgRNA (gfap Zebrafish)

Use

In vitro rna transcription

Promoter

T7

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only

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acta1-Cavin4b-Clover

Plasmid

#163854

Purpose

Zebrafish muscle specific expression of Cavin4b-Clover. Parton lab clone DQZ

Depositor

Rob Parton

Publication

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Cavin4b (cavin4b Zebrafish)

Use

Zebrafish expression

Tags

Clover

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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acta1-Cavin1a-Clover

Plasmid

#163852

Purpose

Zebrafish muscle specific expression of Cavin1a-Clover, Parton lab clone DPR

Depositor

Rob Parton

Publication

Xiong et al Elife. 2021 Feb 16;10. pii: 64631. doi:

Insert

Cavin1a (cavin1a Zebrafish)

Use

Zebrafish expression

Tags

Clover

Available since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pHD066

Plasmid

#84032

Purpose

Vector for creating transgenic zebrafish. Expresses mCherry-Cre fusion protein under the elastase promoter and Venus under the crystalin promoter in zebrafish

Depositor

Daniel Hesselson, Didier Stainier

Publication

Hesselson et al Curr Biol. 2011 Apr 26;21(8):712-7. doi:

Inserts

Cre
Venus

Use

Cre/Lox; Zebrafish transgenesis

Tags

mCherry

Promoter

Crystalin and Elastase

Available since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pUC19-TgKI-MCS-mLanYFP-polyA

Plasmid

#174021

Purpose

This plasmid is used for generating C-terminal knock-in plasmid and/or PCR donors in zebrafish.

Depositor

Michel Bagnat

Publication

Levic et al Development. 2021 Sep 8. pii: 272090. do

Insert

mLanYFP

Use

Zebrafish knock-in tagging

Available since

Nov. 22, 2021

Availability

Academic Institutions and Nonprofits only

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pISceI Dusp6:D2EGFP

Plasmid

#32667

Depositor

Michael Tsang

Publication

Molina et al BMC Dev Biol. 2007 Jun 6;7:62.

Insert

Dusp6 promoter (dusp6 Zebrafish)

Use

Zebrafish transgenesis

Tags

EGFP

Available since

Nov. 28, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTwist-ENTR-NP_001373296.1

Plasmid

#192750

Purpose

Gateway entry vector encoding zebrafish NP_001373296.1

Depositor

Summer Thyme

Insert

rbm11 (rbm11 Zebrafish)

Use

Gateway Cloning

Promoter

None

Available since

Nov. 21, 2022

Availability

Academic Institutions and Nonprofits only

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