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pAAV-hSyn-iLMO (slGLuc-Mac-EYFP)

Plasmid

#114100

Purpose

fusion protein of Gaussia luciferase variant superluminescent, Leptosphaeria maculans opsin, and enhanced yellow fluorescent protein for bioluminescent optogenetics

Depositor

Ute Hochgeschwender

Article

Berglund et al Proc Natl Acad Sci U S A. 2016 Jan 19;11

Insert

Gaussia luciferase, superluminescent; Leptosphaeria maculans opsin; EYFP

Use

AAV

Expression

Mammalian

Promoter

hSyn

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pCR-HA-IKKalphad17

Plasmid

#19738

Depositor

Hiroyasu Nakano

Article

Nakano et al Proc Natl Acad Sci U S A. 1998 Mar 31. 9

Insert

IkB kinase alpha (Chuk Mouse)

Tags

HA

Expression

Mammalian

Mutation

Splice variant lacking 17 amino acids at the C-te…

Available Since

Nov. 12, 2008

Availability

Academic Institutions and Nonprofits only
pET21a-MKP7_K55R_153

Plasmid

#37794

Depositor

SeWon Suh

Article

Kim et al Proc Natl Acad Sci U S A. 2012 May 15;10

Insert

Mitogen-activated protein kinase phosphatase 7 (DUSP16 Human)

Tags

His

Expression

Bacterial

Mutation

changed Lysnie 55 to Arginine and deleted amino a…

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only
pGEX4T2 gp78 CD-m1,2 / JM25

Plasmid

#13307

Depositor

Allan Weissman

Article

Chen et al Proc Natl Acad Sci U S A. 2006 Jan 10. 1

Insert

gp78 (AMFR Human)

Tags

GST

Expression

Bacterial

Mutation

cDNA corresponds to aa309-643 derived from human …

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_hE2F7mg_2ntmut

Plasmid

#73074

Purpose

human E2F7 minigene (exons 11,12,13/introns cassette) with mutated snord27 binding site

Depositor

Stefan Stamm

Article

Falaleeva et al Proc Natl Acad Sci U S A. 2016 Mar 22;11

Insert

E2F7 (E2F7 Human)

Expression

Mammalian

Mutation

partial gene, exons 11,12,13 spaced by introns, 2…

Available Since

Dec. 7, 2018

Availability

Academic Institutions and Nonprofits only
pUBQ10::ZF108_3xFLAG_TET1cd

Plasmid

#106432

Purpose

pUBQ10 driven Zinc Finger human TET1 catalytic domain fusion that targets the FWA promoter in arabidopsis

Depositor

Steven Jacobsen

Article

Gallego-Bartolome et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

Insert

pUBQ10::ZF108_3xFLAG_TET1cd (TET1 Mustard Weed, Synthetic, Human)

Use

Synthetic Biology

Expression

Plant

Available Since

March 14, 2018

Availability

Academic Institutions and Nonprofits only

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pSLIK-Venus-TmiR-G12

Plasmid

#25740

Purpose

Lentiviral vector with Tet-based inducible expression of mouse G alpha 12 miR30-based shRNA, constitutive Venus fluorescent protein coexpression.

Depositor

Iain Fraser

Article

Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

Insert

G alpha 12 miR-shRNA (Gna12 Mouse)

Use

Lentiviral and RNAi

Tags

Venus

Expression

Mammalian

Available Since

July 1, 2010

Availability

Academic Institutions and Nonprofits only

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pFB-Flag-MyoD(QQQ)-E12

Plasmid

#26003

Depositor

Jeffrey Dilworth

Article

Dilworth et al Proc Natl Acad Sci U S A. 2004 Aug 10. 1

Insert

MyoD(QQQ)-E12 Heterodimer Fusion (Myod1 Mouse)

Use

Baculovirus transfer vector

Tags

Flag

Mutation

Changed Lysine 99 to Glutamine; Lysine 102 to Glu…

Available Since

Sept. 8, 2010

Availability

Academic Institutions and Nonprofits only

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MAP4K3-GFP d344-873, K45E

Plasmid

#21735

Depositor

L. Miguel Martins

Article

Lam et al Proc Natl Acad Sci U S A. 2009 Jul 21;10

Insert

MAP4K3 (MAP4K3 Human)

Tags

GFP

Expression

Mammalian

Mutation

Deletion of the carboxy-terminus, amino-acids 344…

Available Since

June 3, 2010

Availability

Academic Institutions and Nonprofits only
pSico-syn-GFP-TVA-oG-WPRE-bGHpA

Plasmid

#231009

Purpose

Lentiviral helper virus for G-deleted rabies tracing

Depositor

Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Inserts

Enhanced GFP
TVA (CD320 Chicken)
optimized G

Use

Lentiviral

Promoter

human synapsin I

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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CVS-N2c-RVdG-MCP:oScarlet:KASH-6xMBS

Plasmid

#231011

Purpose

CVS N2c RVdG genome plasmid. Utilizes MS2 tagging to deliver the MCP:oScarlet:KASH transcript to the outer nuclear membrane. Insert high complexity barcode between SacII RE sites distal to 6xMBS site.

Depositor

Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Insert

MCP-oScarlet-KASH-6xMBS-SacIIMCS

Use

Neurotropic virus

Tags

KASH and MCP

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSicoFlipped-CAG-GFP-WPRE-bGHpA

Plasmid

#231018

Purpose

For strong lentivirus-mediated expression of GFP

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

Enhanced GFP

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pSicoFlipped-CAG-dTomato-WPRE-bGHpA

Plasmid

#231019

Purpose

For strong lentivirus-mediated expression of dTomato

Depositor

Tomasz Nowakowski

Article

Shin et al Cell Stem Cell. 2024 Mar 7;31(3):421-432

Insert

dTomato

Use

Lentiviral

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_S273C

Plasmid

#248864

Purpose

Expresses MBP-tagged C-terminal domain of TDP-43 with S273C.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_S273C (TARDBP Human)

Tags

6xHis tags-MBP

Expression

Bacterial

Mutation

changed S273C

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_M337A

Plasmid

#248885

Purpose

Edit Expresses 6xHis-tagged C-terminal domain of TDP-43 with M337A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_M337A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed M337A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_M339A

Plasmid

#248886

Purpose

Edit Expresses 6xHis-tagged C-terminal domain of TDP-43 with M339A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_M339A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed M339A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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TDP-43_CTD_L340A

Plasmid

#248887

Purpose

Edit Expresses 6xHis-tagged C-terminal domain of TDP-43 with L340A.

Depositor

Nicolas Fawzi

Article

Rizuan et al Nat Commun. 2025 Nov 26;16(1):10528. doi

Insert

TDP-43_CTD_L340A (TARDBP Human)

Tags

6xHis-tags

Expression

Bacterial

Mutation

changed L340A

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pTW411c

Plasmid

#246584

Purpose

different combination of A. thaliana and N. tabacum Rubisco assembly factors

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

NtRaf1, NtRaf2, NtRbcX2, AtBSD2, AtCpn60alpha, AtCpn60beta, AtCpn20 (CPN60B, CPN60A, CPN20 Mustard Weed)

Expression

Bacterial

Available Since

Oct. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hsynapsin-DIO-scramble shRNA - mCherry

Plasmid

#245064

Purpose

AAV transgene designes to express scramble (control) shRNA sequence cell-type selectively. Scramble hairpin expressed from the mir30 cassette together with mCherry.

Depositor

William Wisden

Article

Anuncibay Soto et al Proc Natl Acad Sci U S A. 2025 Oct 14;12

Insert

AAV-DIO-scramble-shRNA-mCherry

Use

AAV, Cre/Lox, and RNAi

Tags

mCherry

Expression

Mammalian

Promoter

human synapsin

Available Since

Oct. 15, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-ETV1_DBD(332-458)

Plasmid

#188052

Purpose

Expresses His8-ETV1_DBD(332-458) with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

ETV1 (ETV1 Human)

Tags

His8

Expression

Bacterial

Mutation

deleted amino acids 1-331, 459-477

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pAG8Ha-EWS

Plasmid

#188044

Purpose

Expresses His8-EWS with a TEV site for removal of the His8 tag.

Depositor

David Libich

Article

Selig et al Proc Natl Acad Sci U S A. 2025 May 20;12

Insert

EWSR1 (EWSR1 Human)

Tags

His8

Expression

Bacterial

Promoter

T7

Available Since

June 9, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Endo-InPGrp

Plasmid

#236003

Purpose

Genetically encoded FRET-based phosphoinositide sensor for monitoring PI(3,4,5)P3. Targeted to endosomes.

Depositor

Jin Zhang

Article

Sahan et al Proc Natl Acad Sci U S A. 2025 Apr;122(1

Insert

Endo-InPGrp (Cyth3 Rat, Synthetic)

Tags

2xFYVE (Fab1/YOTB/Vac1/EEA1 zinc-finger), 6xHIS -…

Expression

Mammalian

Promoter

CMV

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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MZB053

Plasmid

#217835

Purpose

Bacterial expression of human AP3B1 (1-677) and AP3M1 AP-3 core hemicomplex

Depositor

Richard Baker

Article

Begley et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Inserts

AP3B1 (AP3B1 Human)
AP3M1 (AP3M1 Human)

Tags

GST

Expression

Bacterial

Mutation

Removed residues 678-1094 from AP3B1

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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pmNeon Green-N1-ARF1N/3C-R

Plasmid

#202433

Purpose

Mammalian expression vector containing mNeonGreen-tagged ARF3 C-terminus/ARF1 N-terminus chimera

Depositor

David Bryant

Article

Sandilands et al J Cell Biol. 2023 Apr 3;222(4):e20220611

Insert

ARF1 (ARF1 Human)

Use

Lentiviral

Tags

mNeonGreen

Mutation

C terminus replaced with ARF3 sequence (S174A/R17…

Available Since

March 5, 2025

Availability

Academic Institutions and Nonprofits only

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pC131: pAAV.CMV-CasRx-VEGFA sgRNA array

Plasmid

#203444

Purpose

Plasmid expressing active RfxCas13d with an array of three VEGFA mRNA targeting gRNAs

Depositor

Guei-Sheung Liu

Article

Kumar et al Proc Natl Acad Sci U S A. 2024 Nov 5;121

Inserts

U6-VEGFA sgRNA array
RfxCas13d

Use

AAV

Tags

HA and NLS

Expression

Mammalian

Promoter

CMV and U6

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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mTph2p(330bp)-Luc

Plasmid

#223663

Purpose

Firefly-luciferase reporter expression driven by proximal 330-bp of mouse Tph2 promoter

Depositor

Kyungjin Kim

Article

Park et al Commun Biol. 2024 Aug 15;7(1):998. doi:

Insert

mTph2 promoter (Tph2 Mouse)

Use

Luciferase

Tags

Firefly luciferase

Expression

Mammalian

Promoter

mTph2 promoter

Available Since

Sept. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-ILF3-ts1

Plasmid

#174265

Purpose

ILF3 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

ILF3 shRNA (ILF3 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-ILF3-ts2

Plasmid

#174266

Purpose

ILF3 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

ILF3 shRNA (ILF3 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAPM-miR30-ILF3-ts3

Plasmid

#174267

Purpose

ILF3 knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

ILF3 shRNA (ILF3 Human)

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pPU-huMDA5-miR30-L1221

Plasmid

#174223

Purpose

All-in-one huMDA5 rescue-control shRNA knockdown vector

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MDA5 (IFIH1 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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