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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,741 results for: BLI

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Showing: 3981 - 4000 of 9741 results

  1. pBV514

    Plasmid
    #204602
    Purpose
    Expresses F74A OsTir1-3X Myc from S. cerevisiae ADH1 promoter
    Depositor
    Scott Moye-Rowley
    Insert
    Os Tir1 (LOC4335696 Oryza sativa)
    Tags
    Myc epitope tags
    Expression
    Bacterial
    Mutation
    Changed phenylalanine 74 into an alanine (F74A).
    Promoter
    ScADH1
    Available Since
    July 11, 2024
    Availability
    Academic Institutions and Nonprofits only
    204602-plasmid-map-sequence-id-417406

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  2. pUS252y

    Plasmid
    #191834
    Purpose
    Expresses fuYFP constitutively in E.coli cells. Designed to act as modular template for cutting out or amplifying fuYFP to place in other plasmid backbones.
    Depositor
    Nicholas Coleman, Mark Somerville
    Insert
    fuYFP
    Expression
    Bacterial
    Available Since
    Jan. 17, 2023
    Availability
    Industry, Academic Institutions, and Nonprofits
    191834-plasmid-map-sequence-id-382126

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  3. MSCV-FlagmuEzh2deltaset-Hygro

    Plasmid
    #49403
    Purpose
    Expresses a Delta-set deletion mutant of Flag Tagged Ezh2. Hygromycin resistant.
    Depositor
    Tobias Neff
    Insert
    3prime end of Flag-mus-Ezh2deltaSET, eliminating Set domain
    Use
    Retroviral
    Tags
    Flag
    Expression
    Mammalian
    Mutation
    Set domain deleted
    Promoter
    MSCV promotor
    Available Since
    Dec. 9, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. SNTB2

    Plasmid
    #38912
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SNTB2 (SNTB2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  5. pLEW-pHluorin2-PTS1

    Plasmid
    #213776
    Purpose
    Expresses the fluorescent protein pH sensor pHlorin2 in the glycosomes of Trypanosomal brucei
    Depositor
    Kenneth Christensen
    Insert
    pHlorin2
    Use
    Trypanosoma brucei expression
    Mutation
    Appended a 2 glycine linker and a T. brucei perox…
    Promoter
    T. brucei rRNA promotor
    Available Since
    Feb. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    213776-plasmid-map-sequence-id-423197

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  6. pRFP.mIKKε

    Plasmid
    #74414
    Purpose
    Expresses mouse RFP-IKKε in mammalian cells
    Depositor
    Georgios Stathopoulos
    Insert
    mouse IKKε (Ikbke Mouse)
    Tags
    RFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    74414-plasmid-map-sequence-id-140322

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  7. PIM2

    Plasmid
    #38867
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PIM2 (PIM2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  8. SEPX1

    Plasmid
    #39048
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    SEPX1 (MSRB1 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    SeCys95-S
    Available Since
    May 7, 2013
    Availability
    Academic Institutions and Nonprofits only

  9. HaloTag-G protein beta-2

    Plasmid
    #67017
    Purpose
    G protein beta-2 tagged with HaloTag on amino terminus
    Depositor
    Catherine Berlot
    Insert
    NM_005273.3 (GNB2 Human)
    Tags
    HaloTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 30, 2015
    Availability
    Academic Institutions and Nonprofits only
    67017-plasmid-map-sequence-id-115479

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  10. PIAS2

    Plasmid
    #40743
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    PIAS2 (PIAS2 )
    Tags
    6xHis TEV
    Expression
    Bacterial
    Available Since
    Oct. 18, 2012
    Availability
    Industry, Academic Institutions, and Nonprofits

  11. RRAS2

    Plasmid
    #38816
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    RRAS2 (Rras2 Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  12. pcU6.3_gRNA_c1

    Plasmid
    #221381
    Purpose
    Chicken-specific U6 sgRNA expression mini-vector with 10x capture sequence 1
    Depositor
    James Briscoe, Tatjana Sauka-Spengler
    Type
    Empty backbone
    Use
    CRISPR
    Promoter
    G. gallus U6.3
    Available Since
    Nov. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    221381-plasmid-map-sequence-id-454411

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  13. p2B-57

    Plasmid
    #177927
    Purpose
    Expresses yeGFP under control of doxycycline-responsive TET3 promoter (contains three rtTA binding sites)
    Depositor
    Vadim Gladyshev
    Article
    Barre et al (unpublished)
    Insert
    EGFP
    Expression
    Bacterial and Yeast
    Promoter
    TET3 (contains three rtTA binding sites)
    Available Since
    Dec. 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    177927-plasmid-map-sequence-id-349993

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  14. 4LG1

    Plasmid
    #60092
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Cheryl Arrowsmith
    Insert
    METTL21D:JMC072-C04:C227980 (VCPKMT Human)
    Tags
    6xHis
    Expression
    Bacterial
    Mutation
    See comments
    Promoter
    T7
    Available Since
    Oct. 22, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  15. pDual_dsCas9_Blast_hs_NC_chr1

    Plasmid
    #214690
    Purpose
    Lentiviral expression vector for an inducible Cas9-P2A-Blasticidin resistance casette with two sgRNA sequences against human non-coding DNA region of chromosome 1 (negative control)
    Depositor
    Kevin Janes
    Insert
    dgRNA_Chr1
    Use
    CRISPR and Lentiviral
    Available Since
    Dec. 19, 2024
    Availability
    Academic Institutions and Nonprofits only
    214690-plasmid-map-sequence-id-428775

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  16. lentiCRISPRv2_PROX1_1+3

    Plasmid
    #121176
    Purpose
    Lentiviral construct for the expression of Cas9 protein and puromycin resistance from EFS promoter and two guide RNAs targeting for deletion PROX1 start codon sequence.
    Depositor
    Timo Otonkoski
    Insert
    Cas9-P2A-puro and U6 promoters driven expression of gRNAs
    Use
    CRISPR and Lentiviral
    Tags
    P2A-puro
    Expression
    Mammalian
    Available Since
    July 18, 2019
    Availability
    Academic Institutions and Nonprofits only
    121176-plasmid-map-sequence-id-235001

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  18. PTPRK

    Plasmid
    #38968
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    PTPRK (PTPRK Human)
    Tags
    His Tev
    Expression
    Bacterial
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. CMV-dCas9_puroR

    Plasmid
    #219825
    Purpose
    CMV-driven expression of dCas9 with puromycin resistance (Adapted from plasmid #68416)
    Depositor
    Philipp Maass
    Insert
    dCas9
    Use
    CRISPR
    Expression
    Mammalian
    Mutation
    D10A/H841A
    Promoter
    CMV
    Available Since
    July 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    219825-plasmid-map-sequence-id-441124

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  20. pMEF2D-3XFlag

    Plasmid
    #32965
    Purpose
    For mammalian expression of mouse MEF2D with a 3x FLAG tag on the C-terminus.
    Depositor
    Ron Prywes
    Insert
    MEF2D (Mef2d Mouse)
    Tags
    Flag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 2, 2012
    Availability
    Academic Institutions and Nonprofits only
Showing: 3981 - 4000 of 9741 results