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pDONR-221-P3-P4

Plasmid

#186351

Purpose

Gateway donor vector with AttP3 AttP4 recombination sites

Depositor

Patrick Lemaire

Article

Roure et al PLoS One. 2007. 2(9):e916.

Type

Empty backbone

Use

Gateway donor vector

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pTatABCmNeon

Plasmid

#178465

Purpose

pTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatC.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

TatA, TatB, TatCmNeon

Tags

mNeonGreen fused to TatC

Expression

Bacterial

Promoter

araC

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Raptor_1 shRNA

Plasmid

#1857

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

Raptor (RPTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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Rictor_1 shRNA

Plasmid

#1853

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

Rictor (RICTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

July 10, 2005

Availability

Academic Institutions and Nonprofits only

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pET-28a mScarlet

Plasmid

#178543

Purpose

The mScarlet fluorescent protein with an N-terminal 6xHis-tag in pET28a.

Depositor

Siegfried Musser

Article

Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

Insert

mScarlet

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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Rictor_2 shRNA

Plasmid

#1854

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

Rictor (RICTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 7, 2006

Availability

Academic Institutions and Nonprofits only

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Raptor_2 shRNA

Plasmid

#1858

Depositor

David Sabatini

Article

Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

Insert

Raptor (RPTOR Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

April 5, 2006

Availability

Academic Institutions and Nonprofits only

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pBABE zeo PPP2CA Wt

Plasmid

#10689

Depositor

William Hahn

Article

Chen et al Cancer Cell. 2004 Feb . 5(2):127-36.

Insert

protein phosphatase 2, catalytic subunit, alpha isozyme (PPP2CA Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only

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pAW203

Plasmid

#113243

Purpose

E. coli expression vector for IHFa E10A + IHFb

Depositor

Jennifer Doudna

Article

Wright et al Science. 2017 Sep 15;357(6356):1113-1118

Inserts

IHFa E10A (ihfA E. coli)
IHFb

Expression

Bacterial

Mutation

E10A

Available Since

Aug. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pLenti-Cre-MS2_UbC-GFP (Cre_GFP)

Plasmid

#219536

Purpose

a. Expresses Cre recombinase tagged with 21x repeats of MS2 binding sequence under EF1A promotor and b. GFP reporter under UbC promotor

Depositor

Jeffrey Gerst

Article

Haimovich et al bioRxiv 2024.11.06.622258

Inserts

Cre recombinase (cre )
GFP

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 11, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pCB301-2μ-HDV

Plasmid

#196329

Purpose

A yeast-Escherichia coli-Agrobacterium shuttle vector

Depositor

Zhenghe Li

Article

Sun et al Viruses. 2017 Nov 7;9(11):332. doi: 10.3

Type

Empty backbone

Expression

Plant

Available Since

April 12, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA5_FRT_GFP_12AAA_mCH

Plasmid

#127111

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

GFP-mCherry fusion

Expression

Mammalian

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 - hSRSF6

Plasmid

#120419

Purpose

overexpression of human SRSF6

Depositor

Gillian Bates, Andreas Neueder

Article

Neueder et al Nat Commun. 2018 Sep 27;9(1):3955. doi:

Insert

SRSF6 (SRSF6 Human)

Expression

Mammalian

Promoter

CMV immediate/early

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pBY2982

Plasmid

#23220

Depositor

Ralf Baumeister

Article

Schaffer et al Nucleic Acids Res. 2010 Apr;38(6):e91. d

Insert

BirA-mCherry

Expression

Mammalian

Available Since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only

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PlayBack-Ef1aKpn1

Plasmid

#203307

Purpose

For expression of eukaryotic genes of interest with Ef1a promoter with Kpn1 as a PlayBack Restriction Enzyme

Depositor

Aleksandar Necakov

Article

Foran et al Biotechniques. 2023 Oct;75(4):168-178. d

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA-D3cpv

Plasmid

#36323

Depositor

Amy Palmer, Roger Tsien

Article

Palmer et al Chem Biol. 2006 May;13(5):521-30.

Insert

D3cpv second generation cameleon (calcium sensor)

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2012

Availability

Academic Institutions and Nonprofits only

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pBEVY-GU

Plasmid

#51229

Purpose

bi-directional expression vector for yeast, galactose inducible, URA3 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GAL1,10

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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pOGG039

Plasmid

#113996

Purpose

SC module. Terminator for T7RNAP

Depositor

Philip Poole

Article

Geddes et al Front Microbiol. 2019 Jan 14;9:3345. doi

Insert

Terminator for T7RNAP

Expression

Bacterial

Mutation

Domesticated for Golden Gate cloning

Available Since

Jan. 16, 2019

Availability

Academic Institutions and Nonprofits only

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gag-TadCBEd

Plasmid

#225144

Purpose

Packaging plasmid for TadCBEd in v3 PE-eVLP architecture

Depositor

David Liu

Article

An et al Nat Biotechnol. 2024 Jan 8. doi: 10.1038

Insert

MMLVgag-TadCBEd

Expression

Mammalian

Available Since

Sept. 17, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-ldhGFP

Plasmid

#27170

Depositor

Michela Lizier

Article

Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

Insert

lactate dehydrogenase promoter region fused with modified green fluorescent protein 5

Expression

Bacterial

Available Since

Jan. 24, 2011

Availability

Academic Institutions and Nonprofits only

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