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pBacPAK8-His-VHL-Flag(117-213)

Plasmid

#29501

Depositor

Joan Conaway, Ronald Conaway

Article

Kamura et al Proc Natl Acad Sci U S A. 2000 Sep 12. 9

Insert

Von Hippel-Lindau (VHL Human)

Tags

Flag and His

Expression

Insect

Mutation

VHL(117-213) only

Available Since

April 27, 2011

Availability

Academic Institutions and Nonprofits only
pET151-hUCH37 (1-228)

Plasmid

#61932

Purpose

Expression of hUCH37 (1-228) in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Insert

hUCH37 (1-228) (UCHL5 Human)

Tags

6x HIS

Expression

Bacterial

Mutation

Stop codon introduced after residue K228

Promoter

T7

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pHL1855

Plasmid

#53041

Purpose

PLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st3):csrA

Depositor

Han Lim

Article

Adamson et al Proc Natl Acad Sci U S A. 2013 Aug 6;110

Inserts

GFP
tetR
csrA

Use

Synthetic Biology

Tags

LVA, RBS(st3), and glgC leader

Expression

Bacterial

Promoter

pConNoHind and pLlac0-1

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only
pGEX Itk TEC

Plasmid

#20711

Depositor

Leslie Berg

Article

Bunnell et al J Biol Chem. 2000 Jan 21. 275(3):2219-30

Insert

Itk TEC (Itk Mouse)

Tags

GST

Expression

Bacterial

Mutation

amino acids 97-174

Available Since

March 17, 2009

Availability

Academic Institutions and Nonprofits only
HOXB4-NA-MSCV

Plasmid

#20987

Depositor

Corey Largman

Article

Krosl et al Oncogene. 1998 Jul 2. 16(26):3403-12.

Insert

HOXB4 (HOXB4 Human)

Use

Retroviral

Expression

Mammalian

Mutation

N51A (makes DNA binding mutant protein)

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
MET FLAG46QDProCFPp303

Plasmid

#15589

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 46Q Delta Pro (HTT Human)

Tags

CFP and FLAG

Expression

Yeast

Mutation

Proline-rich region removed

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
GAL HA25Q+ProCFPp303

Plasmid

#15570

Depositor

Susan Lindquist

Article

Duennwald et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

Insert

htt 25Q + Pro (HTT Human)

Tags

CFP and HA

Expression

Yeast

Mutation

Proline-rich region present

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
pEF Itk Y511F myc

Plasmid

#20709

Depositor

Leslie Berg

Article

Bunnell et al J Biol Chem. 2000 Jan 21. 275(3):2219-30

Insert

Itk Y511F (Itk Mouse)

Tags

myc

Expression

Mammalian

Mutation

Y511F.

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
H1N1-PR8_RBD

Plasmid

#251261

Purpose

To express the H1N1-PR8 influenza receptor binding domain in E. coli

Depositor

Yutaka Kuroda

Article

Tu et al Int J Mol Sci. 2024 Apr 1;25(7):3943. do

Insert

H1N1-PR8_RBD

Tags

6xHis, 11 aa linker, thrombin cleavage site

Expression

Bacterial

Available Since

April 27, 2026

Availability

Academic Institutions and Nonprofits only

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pQE30::CLOMELEON:Strep

Plasmid

#247989

Purpose

Expression of the indicator protein CLOMELEON in E. coli.

Depositor

Christoph Plieth

Article

Lorenzen et al Plant J. 2004 May;38(3):539-44. doi: 10.

Insert

CLOMELEON (CFP-YFP fusion with ENLYFQG-linker)

Tags

6xHis and Strep

Expression

Bacterial

Mutation

CFP-variant: K26R, F64L, S65T, Y66W, N146I, M153T…

Promoter

T5-phage promoter

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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pmRNA-iEMCV-EGFP-A120

Plasmid

#225905

Purpose

In vitro transcription of cap-independently (EMCV IRES) translated mRNA encoding EGFP.

Depositor

Tom Brown

Article

Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

Inserts

A-inserted T7 class III Φ6.5 promoter
EMCV IRES
EGFP
2x β-globin UTR
poly(A) tail

Use

Synthetic Biology; Mrna preparation

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pmRNA-iHCV-EGFP-A120

Plasmid

#225906

Purpose

In vitro transcription of cap-independently (HCV IRES) translated mRNA encoding EGFP.

Depositor

Tom Brown

Article

Golojuch et al Nat Commun. 2025 Oct 16;16(1):9205. doi:

Inserts

A-inserted T7 class III Φ6.5 promoter
HCV IRES
EGFP
2x β-globin UTR
poly(A) tail

Use

Synthetic Biology; Mrna preparation

Expression

Mammalian

Available Since

Oct. 23, 2025

Availability

Academic Institutions and Nonprofits only

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ADH1pro_LEU3_SIR4

Plasmid

#190582

Purpose

Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

Depositor

Rob Mitra

Article

Recio et al Nucleic Acids Res. 2023 Jun 9;51(10):500

Insert

LEU3 (LEU3 Budding Yeast)

Expression

Yeast

Promoter

ADH1

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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ADH1pro_LYS14_SIR4

Plasmid

#190583

Purpose

Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

Depositor

Rob Mitra

Article

Recio et al Nucleic Acids Res. 2023 Jun 9;51(10):500

Insert

LYS14 (LYS14 Budding Yeast)

Expression

Yeast

Promoter

ADH1

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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ADH1pro_TEA1_SIR4

Plasmid

#190586

Purpose

Transcription factor tagged with Sir4 used for Calling Cards experiments for binding measurement

Depositor

Rob Mitra

Article

Recio et al Nucleic Acids Res. 2023 Jun 9;51(10):500

Insert

TEA1 (TEA1 Budding Yeast)

Expression

Yeast

Promoter

ADH1

Available Since

Oct. 3, 2025

Availability

Academic Institutions and Nonprofits only

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pSplit-SWITCHER1-TF1-dsRED

Plasmid

#213044

Purpose

A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

Split-SWITCHER1-TF1-dsRED

Use

CRISPR

Expression

Mammalian

Promoter

cBH

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pSplit-SWITCHER2-TF1-dsRED

Plasmid

#213045

Purpose

A CRISPR/Cas12a-inducible Split-Cre construct with dsRED as a reporter after the Lox-Stop-Lox cassette

Depositor

Petr Bartunek

Article

Schuster et al Commun Biol. 2025 Jul 2;8(1):982. doi: 1

Insert

Split-SWITCHER1-TF1-dsRED

Use

CRISPR

Expression

Mammalian

Promoter

CBh

Available Since

July 1, 2025

Availability

Academic Institutions and Nonprofits only

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pDL0_029

Plasmid

#210761

Purpose

SC_Oryza sativa codon-optimized LHGR-N(Ligand binding domain and rat glucocorticoid receptor (rcoLHGR-N)

Depositor

Dmitri Nusinow

Article

Lee et al Plant Biotechnol J. 2025 Dec 5. doi: 10.

Insert

rice codn optimized LHGRN

Use

Synthetic Biology

Expression

Plant

Available Since

June 25, 2025

Availability

Academic Institutions and Nonprofits only

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mproSAAS (27 kDa)

Plasmid

#231633

Purpose

Mammalian expression of the full-length mouse chaperone protein proSAAS.

Depositor

Iris Lindberg

Article

Fortenberry et al J Biol Chem. 2002 Feb 15;277(7):5175-86.

Insert

Pcsk1N (Pcsk1n Mouse)

Expression

Mammalian

Available Since

June 20, 2025

Availability

Academic Institutions and Nonprofits only

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pHR-PUM.HD-mCherry-Cry2WT

Plasmid

#240102

Purpose

Mammalian lentiviral vector for expressing the mCherry-tagged Pumilio homology domain fused to the light-inducible oligomerization domain CRY2.

Depositor

Liam Holt

Article

Xie et al Mol Cell. 2024 Jul 25;84(14):2698-2716.e

Insert

Pumilio homology domain

Use

Lentiviral

Tags

mCherry and Cry2

Expression

Bacterial and Mammalian

Promoter

SFFV promoter

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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