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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 14,067 results for: LEA

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Showing: 3981 - 4000 of 14067 results

  1. pGBW-m4134096

    Plasmid
    #152039
    Purpose
    Mammalian expression plasmid for SARS-CoV-2 M (membrane)
    Depositor
    Ginkgo Bioworks, Benjie Chen
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    SARS-CoV-2 M (membrane) (M Severe acute respiratory syndrome coronavirus 2)
    Tags
    Cleavable TEV;S-tag
    Expression
    Mammalian
    Mutation
    wt
    Promoter
    CMV
    Available since
    May 20, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    152039-plasmid-map-sequence-id-300258

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  5. pCMV-bArrestin2-TevC-P2A-TdTomato

    Plasmid
    #89869
    Purpose
    expresses iTango bArrestin2-TevC component and TdTomato bicistronically
    Depositor
    Hyungbae Kwon
    Publication
    Lee et al Nat Methods. 2017 May;14(5):495-503. doi
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    bArrestin2-TevC-P2A-TdTomato
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    R173H in TdTomato (please see depositor comments …
    Promoter
    CMV
    Available since
    May 26, 2017
    Availability
    Academic Institutions and Nonprofits only
    89869-plasmid-map-sequence-id-177073

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  9. pEMS1382

    Plasmid
    #29182
    Purpose
    Expression of the eGFP reporter gene was detected (positive) in the brain, but not detected (negative) in the eye.
    Depositor
    Elizabeth Simpson
    Publication
    Portales-Casamar et al Proc Natl Acad Sci U S A. 2010 Sep 21. 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Ple183 (S100B Human)
    Use
    Pleiades promoter project [sic, pleaides plieades]
    Tags
    EGFP-NLS
    Available since
    Nov. 15, 2011
    Availability
    Academic Institutions and Nonprofits only
    29182-plasmid-map-sequence-id-13597

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  11. pcDNA3-mCherry-Rheb(C181S)

    Plasmid
    #192437
    Purpose
    Encodes mCherry-tagged, farnesylation-deficient Rheb C181S mutant.
    Depositor
    Jin Zhang
    Publication
    Zhong et al Cell Chem Biol. 2022 Jun 16;29(6):1037-1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    mCherry-Rheb(C181S) (RHEB Human)
    Tags
    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…
    Expression
    Mammalian
    Mutation
    Rheb Cys 181 mutated to Ser; abolishes C-terminal…
    Promoter
    CMV
    Available since
    Dec. 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    192437-plasmid-map-sequence-id-379127

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  12. pcDNA3-AKAP79-(Ci/Ce)-Epac2-camps

    Plasmid
    #181956
    Purpose
    Genetically encoded FRET-based cAMP indicator fused to AKAP79.
    Depositor
    Jin Zhang
    Publication
    Tenner et al Elife. 2020 Nov 17;9. pii: 55013. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AKAP79-(Ci/Ce)-Epac2-camps (AKAP5 Human)
    Tags
    AKAP79, Cerulean (CFP), Citrine (YFP), and HIS ta…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    181956-plasmid-map-sequence-id-363427

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  13. pcDNA3-JNKAR1-NES

    Plasmid
    #84632
    Purpose
    Unimolecular, FRET-based JNK activity reporter; cytosol targeted
    Depositor
    Jin Zhang
    Publication
    Depry et al Chem Biol. 2015 Nov 19;22(11):1470-9. do
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    ECFP-FHA1-JNKsubDD-Citrine-NES
    Tags
    6xHis, Citrine, ECFP, Nuclear export signal (NES)…
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Nov. 14, 2018
    Availability
    Academic Institutions and Nonprofits only
    84632-plasmid-map-sequence-id-176128

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  14. SpCas9-NG-SsAPOBEC3B

    Plasmid
    #198536
    Purpose
    Expresses human codon-optimized SpCas9-NG-SsAPOBEC3B and blasticidin resistance: EFS promoter-SpCas9-NG-SsAPOBEC3B-NLS-FLAG-P2A-BSD
    Depositor
    Hyongbum Kim
    Publication
    Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.
    Insert
    SpCas9-NG-SsAPOBEC3B
    Use
    CRISPR and Lentiviral
    Tags
    BSD, FLAG, and NLS
    Expression
    Mammalian
    Promoter
    EFS
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    198536-plasmid-map-sequence-id-388885

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  15. pCAG_iPE-C

    Plasmid
    #214735
    Purpose
    encodes iPE-C prime editor (with MMLV-RT(H8Y, D200N, T306K, W313F, T330P, L603W) fused to the C terminus of nCas9-N863A) driven by a CAG promoter
    Depositor
    Gil Westmeyer
    Publication
    Truong et al Nat Methods. 2024 Feb 1. doi: 10.1038/s4
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    CAG_iPE-C_bGH
    Use
    CRISPR and Synthetic Biology
    Expression
    Mammalian
    Available since
    March 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    214735-plasmid-map-sequence-id-425000

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Showing: 3981 - 4000 of 14067 results