We narrowed to 15,991 results for: LEA

1. pCAS9counter

   Plasmid

   #107192

   Purpose

   Expresses CAS9 and sgRNA for targeted counterselection in S. aureus

   Depositor

   Steve Salipante

   Article

   Penewit et al MBio. 2018 Feb 20;9(1). pii: mBio.00067-

   Inserts

   Cas9
   sgRNA
   

   Expression

   Bacterial

   Promoter

   prab17

   Available Since

   Feb. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pJH-MBP-CbAgo

   Plasmid

   #127707

   Purpose

   pET His6 MBP TEV CbAgo

   Depositor

   John van der Oost

   Article

   Hegge et al Nucleic Acids Res. 2019 May 9. pii: 5487

   Insert

   CbAgo
   

   Tags

   MPB

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   June 18, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pEJS2700-CMV-Nme2-ABE8e-nt-E932R

   Plasmid

   #228721

   Purpose

   Expresses N-terminal ABE8e-Nme2 nCas9 (D16A) base editor with E932R mutation in mammalian cells

   Depositor

   Erik Sontheimer

   Article

   Bamidele et al bioRxiv November 07, 2024

   Insert

   N-terminal ABE8e with Nme2 nCas9 (D16A) E932R
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Nme2-Cas9 D16A E932R

   Promoter

   CMV

   Available Since

   Nov. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEBFP2-Nuc

   Plasmid

   #14893

   Purpose

   Mammalian expression of EBFP2 blue fluorescent protein with NLS

   Depositor

   Robert Campbell

   Article

   Ai et al Biochemistry. 2007 May 22;46(20):5904-10

   Insert

   pEBFP2-Nuc
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Replace EYFP of the vector pEYFP-Nuc from Clontec…

   Available Since

   May 15, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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5. 2.2

   Plasmid

   #34885

   Depositor

   Irvin Chen

   Article

   Pariente et al Mol Ther. 2007 Nov;15(11):1973-81. Epub

   Insert

   Sindbis virus envelope protein
   

   Use

   Lentiviral

   Expression

   Mammalian

   Mutation

   SGN UTR in E1 domain

   Promoter

   CMV

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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6. p15a_AcCas12n

   Plasmid

   #203806

   Purpose

   p15a-based plasmid containing a rpsL driven AcCas12n cassette and the araBAD-driven lambda-red recombinase cassette. Apramycin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcCas12n
   

   Use

   CRISPR

   Promoter

   rpsl

   Available Since

   Aug. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHBV-Luc

   Plasmid

   #71414

   Purpose

   To see the activity of HBV transcription using Luciferase assay

   Depositor

   Wang-Shick Ryu

   Article

   Cha et al J Gen Virol. 2009 Apr;90(Pt 4):978-86. d

   Insert

   HBV core promoter, enhancer I, enhancer II and luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   HBV

   Available Since

   Dec. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTK305

   Plasmid

   #66812

   Purpose

   IVT vector for Fluc mRNA

   Depositor

   Ron Weiss

   Article

   Andries et al J Control Release. 2015 Sep 2. pii: S016

   Insert

   Firefly luciferase
   

   Use

   Luciferase; Mrna ivt

   Promoter

   T7

   Available Since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1/V5-His Snk/Plk2

   Plasmid

   #16015

   Depositor

   Wafik El-Deiry

   Article

   Burns et al Mol Cell Biol. 2003 Aug . 23(16):5556-71

   Insert

   Snk/Plk2 (PLK2 Human)
   

   Tags

   V5-His

   Expression

   Mammalian

   Available Since

   Nov. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

10. pCMV-BE1

    Plasmid

    #73019

    Purpose

    Expresses BE1 with C-terminal NLS in mammalian cells

    Depositor

    David Liu

    Article

    Komor et al Nature. 2016 Apr 20. doi: 10.1038/nature

    Insert

    BE1
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEJS2712-CMV-eNme2-C-ABE8e-i1-L10

    Plasmid

    #228733

    Purpose

    Expresses domain inlaid eNme2-C nCas9 (D16A)-i1-ABE8e base editor with linker-10 in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    Domain inlaid eNme-C nCas9 (D16A)-i1-L10
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS/ES-EnvA-VSVg-WPRE-pA

    Plasmid

    #87221

    Purpose

    to package wild type EnvA psudotyped lentivirus

    Depositor

    Fan Wang

    Article

    Sakurai et al Neuron. 2016 Nov 23;92(4):739-753. doi:

    Insert

    EnvA-VSVg
    

    Use

    Mouse Targeting and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. 2HA-pDEST-N

    Plasmid

    #118373

    Purpose

    Gateway compatible mammalian expression vector with N-terminal 2HA to tag proteins of interest.

    Depositor

    Maria Vartiainen

    Article

    Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

    Type

    Empty backbone

    Use

    Destination

    Tags

    2HA

    Expression

    Mammalian

    Available Since

    May 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCMV-PV-NES-GFP

    Plasmid

    #17301

    Depositor

    Anton Bennett

    Article

    Pusl et al J Biol Chem. 2002 Jul 26. 277(30):27517-

    Insert

    PV-NES-GFP (Pvalb Rat)
    

    Tags

    Myc

    Expression

    Mammalian

    Available Since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. pEJS2701-CMV-Nme2Smu-ABE8e-nt-E932R

    Plasmid

    #228722

    Purpose

    Expresses N-terminal ABE8e-Nme2Smu nCas9 (D16A) base editor with E932R mutation in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    N-terminal ABE8e with Nme2Smu nCas9 (D16A) E932R
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9, Smu PID, D16A E932R

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEJS2702-CMV-iNme2-ABE8e-nt

    Plasmid

    #228723

    Purpose

    Expresses N-terminal ABE8e-iNme2 nCas9 (D16A) base editor in mammalian cells

    Depositor

    Erik Sontheimer

    Article

    Bamidele et al bioRxiv November 07, 2024

    Insert

    N-terminal ABE8e with iNme2 nCas9 (D16A)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Nme2-Cas9 D16A D844G E868K K870R D873A D911G K929…

    Promoter

    CMV

    Available Since

    Nov. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. CT752

    Plasmid

    #233658

    Purpose

    Flp GFP Reporter; FRT-STOP-FRT-GFP

    Depositor

    Wilson Wong

    Article

    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

    Insert

    FRT-STOP-FRT-GFP
    

    Expression

    Mammalian

    Mutation

    None

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. CT769

    Plasmid

    #233661

    Purpose

    Split Flp on same plasmid; PhyA-Flp-397-Flp-396-FHY1

    Depositor

    Wilson Wong

    Article

    Tous et al Sci Adv. 2025 May 9;11(19):eadt1971. doi

    Insert

    PhyA-Flp-397-Flp-396-FHY1
    

    Expression

    Mammalian

    Mutation

    None

    Available Since

    May 12, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. AAV-PS Intein

    Plasmid

    #242020

    Purpose

    AAV version of PS Intein palsmid.

    Depositor

    Vladislav Verkhusha

    Article

    Baloban et al Nat Commun. 2025 Sep 11;16(1):8263. doi:

    Insert

    PS Intein
    

    Use

    Adenoviral

    Tags

    mEGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV HDM2(C464A)

    Plasmid

    #12086

    Depositor

    Tyler Jacks

    Article

    Boyd et al Nat Cell Biol. 2000 Sep . 2(9):563-8.

    Insert

    HDM2 (MDM2 Human)
    

    Expression

    Mammalian

    Mutation

    C464A: RING-finger-domain mutant

    Available Since

    June 8, 2006

    Availability

    Academic Institutions and Nonprofits only