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pGGN000

Plasmid

#48865

Purpose

Empty entry vector for creating GreenGate supermodules with plant resistance cassette.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pAA6

Plasmid

#128282

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq- mRFP, parS

Expression

Bacterial

Available Since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pXDC61-FabI

Plasmid

#21842

Depositor

Howard Shuman

Article

Charpentier et al PLoS Pathog. 2009 Jul . 5(7):e1000501.

Insert

fabI

Tags

TEM

Expression

Bacterial

Available Since

Sept. 2, 2009

Availability

Academic Institutions and Nonprofits only

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pPSSUB-101

Plasmid

#26580

Depositor

Ron Weiss

Article

Basu et al Proc Natl Acad Sci U S A. 2004 Apr 27. 1

Insert

destabilized GFP

Expression

Bacterial

Mutation

S2R, C-terminal extension RPAANDENYA(LVA)

Available Since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pTETDuet_WTDHFR_WTTS

Plasmid

#191282

Purpose

Bacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)

Depositor

Kimberly Reynolds

Article

Nguyen et al Nat Commun. 2024 Apr 18;15(1):3351. doi:

Insert

folA, thyA

Expression

Bacterial

Mutation

WT DHFR, WT TYMS

Promoter

T7 (folA), Tet (thyA)

Available Since

June 5, 2023

Availability

Academic Institutions and Nonprofits only

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pGCS-C1m(5xMYC)

Plasmid

#85724

Purpose

Gateway destination vector

Depositor

Hai-Ning Du

Article

Wang et al Acta Biochim Biophys Sin (Shanghai). 201

Type

Empty backbone

Use

Mammalian/avian/xenopus/zebrafish

Tags

5xMYC

Available Since

Jan. 10, 2017

Availability

Academic Institutions and Nonprofits only

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pGGE000

Plasmid

#48860

Purpose

Empty entry vector for creating GreenGate terminator modules.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Type

Empty backbone

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pBLO 9.2_bCas9-CP199

Plasmid

#121496

Purpose

TetR_tetR/tetA promoters_dCas9-CP_CM_P15A

Depositor

David Savage

Article

Oakes et al Nat Biotechnol. 2016 May 2. doi: 10.1038

Type

Empty backbone

Expression

Bacterial

Promoter

p15A

Available Since

March 6, 2019

Availability

Academic Institutions and Nonprofits only

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EYK054

Plasmid

#69512

Purpose

pBAD pduP1-18-gfpmut2-ssrA

Depositor

Danielle Tullman-Ercek

Article

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

GFPmut2

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pVS45

Plasmid

#20059

Depositor

Stephen Sligar

Article

Beck von Bodman et al Proc Natl Acad Sci U S A. 1986 Dec . 83(

Insert

rat hepatic cytochrome b5

Expression

Bacterial

Available Since

Jan. 12, 2009

Availability

Academic Institutions and Nonprofits only
pMRE-Himar-175

Plasmid

#185943

Purpose

Himar transposon delivery plasmid that delivers a red fluorescent mScarlet-I protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mScarlet-I

Expression

Bacterial

Available Since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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lvl0 mTFP1 CTAG

Plasmid

#153400

Purpose

Cyan fluorescent protein with FLAG tag

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

mTFP1 CTAG

Tags

FLAG tag

Expression

Plant

Available Since

Oct. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pKP1141

Plasmid

#160811

Purpose

Expression of TonB protein

Depositor

Kathleen Postle

Article

Gresock et al Front Microbiol. 2011 Oct 12;2:206. doi:

Insert

tonB (tonB Escherichia coli)

Expression

Bacterial

Available Since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pAC-PHYT

Plasmid

#19002

Depositor

Jay Keasling

Article

Smolke et al Metab Eng. 2001 Oct . 3(4):313-21.

Insert

crtE, crtB

Use

Synthetic Biology

Expression

Bacterial

Available Since

Oct. 27, 2008

Availability

Academic Institutions and Nonprofits only
lvl0 G10-90p

Plasmid

#153370

Purpose

Synthetic promoter for plant expression

Depositor

Naomi Nakayama

Article

Andreou et al bioRxiv, March 31, 2021

Insert

G10-90 promoter

Expression

Plant

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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L_EGFP_AC

Plasmid

#185971

Purpose

Expresses EGFP from PlLaco-1 promoter in E.coli Dh5alpha cell. For experiment use Dh5alphaz1

Depositor

Sangram Bagh

Article

Mukhopadhyay et al Biosens Bioelectron. 2020 Nov 1;167:1124

Insert

Pl-LacO1-Egfp

Use

Synthetic Biology

Promoter

PlLacO-1

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMRE-Himar-134

Plasmid

#185925

Purpose

Himar transposon delivery plasmid that delivers a orange fluorescent mOrange2 protein gene

Depositor

Mitja Remus-Emsermann

Article

Stocks et al Bacteria 2022, 1(1), 56-65

Insert

mOrange2

Expression

Bacterial

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pBP

Plasmid

#72947

Purpose

Backbone vector with internal BsmBI removed by site-directed mutagenesis

Depositor

Paul Freemont

Article

Moore et al ACS Synth Biol. 2016 May 2.

Type

Empty backbone

Use

Synthetic Biology

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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CMJ119

Plasmid

#69517

Purpose

pBAD eutE1-20- gfpmut2-ssrA

Depositor

Danielle Tullman-Ercek

Article

Jakobson et al J Biol Chem. 2015 Aug 17. pii: jbc.M115.

Insert

GFPmut2

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCDW114

Plasmid

#70061

Purpose

artificial transcription template "wild-type"

Depositor

Jeff Gelles

Article

Harden et al Proc Natl Acad Sci U S A. 2016 Jan 5. pi

Insert

lambda PR' - repeat cassette - E. coli rpoB - lambda TR'

Available Since

Jan. 7, 2016

Availability

Academic Institutions and Nonprofits only