We narrowed to 7,548 results for: ski

1. pDule2-IBBN (G2)

   Plasmid

   #85501

   Purpose

   Plasmid for incorporating the non-canonical amino acid IBBN with the Mj IBBN (G2) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Peeler et al J Am Chem Soc. 2010 Oct 6;132(39):13575-

   Insert

   IBBN (G2) synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Y32G L65E F108W Q109M D158S R162K

   Promoter

   lpp (constitutive)

   Available since

   March 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX ShcD-SH2

   Plasmid

   #46488

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   ShcD (SHC4 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 512-623

   Promoter

   Tac

   Available since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHS1871

   Plasmid

   #205976

   Purpose

   U6-JAGBLH010000220 Cas8-HNH DR-BpiI-DR

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   Cas8-HNH DR-BpiI-DR
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Feb. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMCL-HA-MAPKK1-G1C [delta(44-51)/S218E/S222D]

   Plasmid

   #40807

   Depositor

   Natalie Ahn

   Article

   Whalen et al Mol Cell Biol. 1997 Apr;17(4):1947-58.

   Insert

   MAP2K1 (MAP2K1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   delta(44-51), S218E, S222D

   Promoter

   CMV

   Available since

   Oct. 18, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pHS1543

   Plasmid

   #205963

   Purpose

   NZ_CP025815 DinG HNH Cascade proteins operon (E. coli codon optimized) with His14-tagged Csf3 in pET45b(+)

   Depositor

   Feng Zhang

   Article

   Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

   Insert

   DinG HNH Cascade with His14-tagged Csf3
   

   Use

   Tags

   14xHis on Csf3

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5 GFP RagB Q99L

   Plasmid

   #112749

   Purpose

   express GFP-RagB (Q99L mutant)

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   GFP-RagB (Q99L) (RRAGB Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Q99L

   Promoter

   Available since

   Oct. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA SF-HERC2 F1

   Plasmid

   #55784

   Purpose

   Expresses N-terminal 3xFLAG/STREP tagged HERC2 (1-969aa)

   Depositor

   David Chan

   Article

   Chan et al J Biol Chem. 2014 May 22. pii: jbc.M114.

   Insert

   3xFLAG tagged HERC2 (1-969aa) (HERC2 Human)
   

   Use

   Tags

   3xFLAG and STREP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 23, 2014

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-CAG-SuperNovaGreen-T2A-dTomato

   Plasmid

   #193798

   Purpose

   Expresses SuperNova Green in the cytosol of mammalian cells, joined by a T2A cleavage site with dTomato

   Depositor

   Xiao Wang

   Article

   Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

   Insert

   SuperNova Green
   

   Use

   AAV

   Tags

   Flag tag

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPHR-iRFP713

   Plasmid

   #103818

   Purpose

   Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   PHR-iRFP713 (CRY2 Mustard Weed)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGEX SH2-B-SH2

    Plasmid

    #46479

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    SH2-B (SH2B1 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 515-639

    Promoter

    Tac

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. PVT1-Tol2

    Plasmid

    #113381

    Purpose

    evaluate FOXA1 DIV motif enhancer activity near 50 kb of PVT1 gene

    Depositor

    Ralf Jauch

    Article

    Wang et al Nucleic Acids Res. 2018 Jun 20;46(11):54

    Insert

    PVT1-enhancer (PVT1 Human)
    

    Use

    Tol2 reporter

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 31, 2018

    Availability

    Academic Institutions and Nonprofits only

12. pLenti CRISPR sgMTHFD2_1

    Plasmid

    #106313

    Purpose

    Express Cas9 and sgRNA targeting MTHFD2

    Depositor

    Richard Possemato

    Article

    Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

    Insert

    sgRNA targeting MTHFD2 (MTHFD2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pHS1517

    Plasmid

    #205980

    Purpose

    FLAG-SV40 NLS-Cas5-HNH from MWEE01000013 Cas5-HNH in pcDNA3.1(+)

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas5-HNH
    

    Use

    Tags

    FLAG-SV40 NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNAS1b split mCherry 14-3-3β ctrl

    Plasmid

    #112031

    Purpose

    Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Insert

    14-3-3β fused to C-terminal split mCherry, phospho-dependent binding ctrl peptide with TAG fused to N-terminal split mCherry
    

    Use

    Tags

    Split mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. GlycophorinB-CD4d3+4-bio

    Plasmid

    #73094

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinB with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Glycophorin B (GYPB Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pGEX Stat6-SH2

    Plasmid

    #46517

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Stat6 (STAT6 Human)
    

    Use

    Tags

    GST and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 510-654

    Promoter

    Tac

    Available since

    July 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGEX Tec-SH2

    Plasmid

    #46522

    Depositor

    Bruce Mayer

    Article

    Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

    Insert

    Tec (TEC Human)
    

    Use

    Tags

    AviTag, GST, and PreScission

    Expression

    Bacterial

    Mutation

    contains amino acids 236-351

    Promoter

    Tac

    Available since

    Oct. 9, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. Semaphorin7A-CD4d3+4-bio

    Plasmid

    #73115

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Semaphorin7A with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Semaphorin 7A (SEMA7A Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pHS1929

    Plasmid

    #205968

    Purpose

    JAGBLH010000220 Cas8-HNH crRNA expression in pCOLADuet-1

    Depositor

    Feng Zhang

    Article

    Altae-Tran et al Science. 2023 Nov 24;382(6673):eadi1910.

    Insert

    Cas8-HNH crRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LENTICRISPR-PGAM1_sgRNA1

    Plasmid

    #201614

    Purpose

    CRISPR/Cas9-mediated gene knock-out

    Depositor

    Alexis Jourdain, Vamsi Mootha

    Article

    Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

    Insert

    PGAM1 (PGAM1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge