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Mouse sgRNA library Gouda in pRDA_118 backbone

Pooled Library

#136987

Purpose

Designed for assays with limited cell numbers with 2 guides per gene. Compatible with John Doench’s Brie mouse genome-wide library.

Depositor

John Doench

Article

DeWeirdt et al Nat Commun. 2020 Feb 6;11(1):752. doi: 1

Expression

Mammalian

Species

Mus musculus

Use

CRISPR and Lentiviral

Available Since

Feb. 24, 2020

Availability

Academic Institutions and Nonprofits only
Human Brunello kinome pooled library, guides 1-4 in lentiGuide-Puro backbone

Pooled Library

#75312

Purpose

Human sgRNA library in backbone lentiGuide-Puro targeting 763 kinase genes and containing 3,052 unique sgRNAs along with 100 non-targeting controls

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Expression

Mammalian

Use

CRISPR and Lentiviral

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
Human CRISPRa sgRNA library Calabrese in backbone XPR_502 (P65 HSF), Set B

Pooled Library

#92380

Purpose

CRISPRa

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Use

Lentiviral

Available Since

Aug. 3, 2017

Availability

Academic Institutions and Nonprofits only
Human CRISPRi sgRNA library Dolcetto in backbone XPR_050, Set B

Pooled Library

#92386

Purpose

Crispri

Depositor

John Doench, David Root

Article

Sanson et al Nat Commun. 2018 Dec 21;9(1):5416. doi:

Use

Lentiviral

Available Since

July 13, 2018

Availability

Academic Institutions and Nonprofits only
4 gRNA concatemer

Plasmid

#84881

Purpose

Empty concatmer vector in which 4 gRNAs can be inserted

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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4 gRNA concatemer

Plasmid

#84881

Purpose

Empty concatmer vector in which 4 gRNAs can be inserted

Depositor

Bon-Kyoung Koo

Article

Andersson-Rolf et al Dev Biol. 2016 Oct 27. pii: S0012-1606(1

Type

Empty backbone

Use

Retroviral

Expression

Mammalian

Available Since

June 12, 2017

Availability

Academic Institutions and Nonprofits only

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pFL-SV40

Plasmid

#115352

Purpose

firefly luciferase (Fluc) reporter gene driven by SV40 promoter

Depositor

Ming-Chih Lai

Article

Lai et al Mol Biol Cell. 2008 Sep;19(9):3847-58. d

Type

Empty backbone

Use

Luciferase

Expression

Mammalian

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pBEVY-GL

Plasmid

#51225

Purpose

bi-directional expression vector for yeast, galactose inducible, LEU2 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GAL1,10

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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AmCyan1-N1

Plasmid

#54616

Purpose

Localization: N1 Cloning Vector, Excitation: 453, Emission: 486

Depositor

Michael Davidson

Type

Empty backbone

Tags

AmCyan1

Expression

Mammalian

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pBEVY-GU

Plasmid

#51229

Purpose

bi-directional expression vector for yeast, galactose inducible, URA3 selection

Depositor

Charles Miller

Article

Miller CA et al Nucleic Acids Res. 1998 Aug 1;26(15):357

Type

Empty backbone

Expression

Yeast

Promoter

GAL1,10

Available Since

March 21, 2014

Availability

Academic Institutions and Nonprofits only

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